Anti-COX IV抗体

Anti-COX IV抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

200147Anti-COX IV Mouse mAbMouse mAbHuman,Mouse,Rat,Hamster,Goat,MonkeyWB,FC,ICC,IP,IHC17

Sensitivity  This antibody detects endogenous levels of COX IV and does not cross-react with related proteins.

Immunogen  A synthetic peptide corresponding to carboxyl terminal residues of human COX IV

Host  Mouse

Clone Number  4D11-B3-E8

Antibody type  Monoclonal antibody

Anti-COX IV抗体Purified method  Affinity purified

Isotype  IgG1

Formulation  Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.

Dilution

WB~~1:1000

ICC~~1:150

FCM~~1:100

Anti-COX IV抗体Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.

Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.

Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.

Anti-COX IV抗体Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8,1:50 diluted),showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).

Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.

Western blot detection of COX IV in Goat muscle,CHO-k1,COS7,3T3,Hela,C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa.