淋巴细胞功能关联抗原2(LFA2)重组蛋白

淋巴细胞功能关联抗原2(LFA2)重组蛋白

来源多抗制备

宿主兔

效价-

Ig类型IgG

纯化方式抗原特异性亲和纯化

标记物无标记物

免疫原-

缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）

性状液体

浓度200ug/ml

且适物种-

淋巴细胞功能关联抗原2(LFA2)重组蛋白应用WB, ICC, IHC-P, IHC-F, ELISA

Polyclonal Antibody to Lymphocyte Function Associated Antigen 2 (LFA2)

Organism Species: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PRODUCT INFORMATION ]

Immunogen: LFA2, Rat

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant LFA2 (Thr27~Leu203) with N-terminal His-Tag

expressed in E.coli.

Accession No.: RPB576Ra01

淋巴细胞功能关联抗原2(LFA2)重组蛋白[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against LFA2. It has been

selected for its ability to recognize LFA2 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

淋巴细胞功能关联抗原2(LFA2)重组蛋白[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant LFA2 (Thr27~Leu203)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,

BPB 0.01%, NaN3 0.02%.

[ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

[ IMAGES ]

Used in Western Blot, Sample:

Recombinant LFA2, Rat

Used in DAB staining on fromalin fixed

paraffin- embedded brain tissue