钙网蛋白(CRT)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量21.6kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Glu18~Ser193 (Accession # P27797) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
等电点-
钙网蛋白(CRT)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Calreticulin (CRT)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Glu18~Ser193
Tags: N-terminal His-Tag
Accession: P27797
Host: E. coli
Subcellular Location: Endoplasmic reticulum lumen.
Cytoplasm, cytosol. Secreted, extracellular space,
extracellular matrix. Cell surface. Sarcoplasmic
reticulum lumen. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 10mM
PBS, pH7.4, containing 1mM DTT, 5% trehalose,
0.01% sarcosyl and preservative.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 21.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
钙网蛋白(CRT)重组蛋白[ USAGE ]
Reconstitute in sterile ddH2O. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
EPA VYFKEQFLDG DGWTSRWIES KHKSDFGKFV LSSGKFYGDE EKDKGLQTSQ
DARFYALSAS FEPFSNKGQT LVVQFTVKHE QNIDCGGGYV KLFPNSLDQT DMHGDSEYNI
MFGPDICGPG TKKVHVIFNY KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VES
钙网蛋白(CRT)重组蛋白[ REFERENCES ]
1. McCauliffe D.P., et al. (1990) J. Clin. Invest. 85:1379-1391.
2. McCauliffe D.P., et al. (1992) J. Biol. Chem. 267:2557-2562.
3. Goshima N., et al. (2008) Nat. Methods 5:1011-1017.
4. Lieu T.-S., et al. (1988) J. Clin. Invest. 82:96-101.
更多
企业名称
上海康朗生物科技有限公司
企业信息已认证
企业类型
信用代码
310112001510759
成立日期
2015-05-29
注册资本
100
经营范围
从事生物科技技术领域内技术开发、技术咨询、技术服务、技术转让,实验分析仪器、化工产品及原料(除危险化学品、监控化学品、烟花爆竹、民用爆竹物品、易制毒化学品)、仪器仪表、环保设备、酒店用品、皮革制品、床上用品、电子产品的销售。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海康朗生物科技有限公司
公司地址
上海市闵行区闵北路88弄1-30号第22栋BV145室
客服电话