甘露糖结合蛋白(MBL)重组蛋白

甘露糖结合蛋白(MBL)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位分泌

预测分子量59.7kDa

实际分子量63kDa(差异分析请参阅说明书)

片段与标签Leu130~Ile248 with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 97%

等电点5.2

甘露糖结合蛋白(MBL)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling

Recombinant Matrix Gla Protein (MGP)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

甘露糖结合蛋白(MBL)重组蛋白[ PROPERTIES ]

Residues: Met1~Lys103

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P08493

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 9.8

Predicted Molecular Mass: 16.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

甘露糖结合蛋白(MBL)重组蛋白[ SEQUENCES ]

The sequence of the target protein is listed below.

MKSLILLAIL AALAVVTLCY ESHESMESYE LNPFINRRNA NTFISPQQRW RAKVQERIRE

RSKPVHELNR EACDDYRLCE RYAMVYGYNA AYNRYFRKRR GTK

[ REFERENCES ]

1. Cancela M.L., et al. (1990) J. Biol. Chem. 265:15040-15048. 2. Kiefer M.C., et al. (1988) Nucleic Acids Res. 16:5213-5213. 3. Chen L., et al. (1990) Oncogene 5:1391-1395. 4. Hale J.E., et al. (1991) J. BioRPB479Hu01 50μg

Recombinant Heparin Binding Epidermal Growth Factor

Like Growth Factor (HBEGF)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Val21~Thr160

Tags: N-terminal His-Tag

Accession: Q99075

Host: E. coli

Subcellular Location: Secreted, extracellular

space. Cell membrane; Single-pass type I

membrane protein. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 9.3

Predicted Molecular Mass: 17.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O. [ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VTGESLERLR RGLAAGTSNP DPPTVSTDQL LPLGGGRDRK VRDLQEADLD LLRVTLSSKP

QALATPNKEE HGKRKKKGKG LGKKRDPCLR KYKDFCIHGE CKYVKELRAP SCICHPGYHG

ERCHGLSLPV ENRLYTYDHT

[ REFERENCES ]

1. Higashiyama S., et al. (1991) Science 251:936-939.

2. Higashiyama S., et al. (1992) J. Biol. Chem. 267:6205-6212.

3. Mitamura T., et al. (1995) J. Biol. Chem. 270:1015-1019.

4. EleniusRPB480Hu01 10μg

Recombinant Mannose Binding Lectin (MBL)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Leu130~Ile248

Tags: Two N-terminal Tags, His-tag and MBP-tag

Tissue Specificity: Liver, Plasm. Subcellular Location: Secreted. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.2

Predicted Molecular Mass: 59.7kDa

Accurate Molecular Mass: 63kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. Gene Sequencing (Extract)

Figure 2. SDS-PAGE278.. Chem. 266:21145-21149.