去唾液酸糖蛋白受体1(ASGPR1)重组蛋白

去唾液酸糖蛋白受体1(ASGPR1)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU per 1μg (determined by the LAL method).

亚细胞定位细胞膜

预测分子量28.2kDa

实际分子量31kDa(差异分析请参阅说明书)

片段与标签Phe77~Pro289 with N-terminal His Tag

缓冲液成份100mM NaHCO3, 500mM NaCl缓冲液（pH8.3,含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）

性状冻干粉

纯度> 95%

等电点5.8

去唾液酸糖蛋白受体1(ASGPR1)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.

Recombinant Asialoglycoprotein Receptor 1 (ASGR1)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

去唾液酸糖蛋白受体1(ASGPR1)重组蛋白[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Phe77~Pro289

Tags: N-terminal His-Tag

Homology: Mouse 98%, rat 80%

Tissue Specificity: Liver, brain, lung, blood. Subcellular Location: Membrane; Single-pass type II membrane protein. Secreted. Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM

EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.8

Predicted Molecular Mass: 28.2kDa

Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions.

去唾液酸糖蛋白受体1(ASGPR1)重组蛋白[ USAGE ]

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of

0.1-1.0 mg/mL. Do not vortex. [ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. Gene Sequencing (Extract)

Figure 2. SDS-PAGE