过氧化物酶体增殖物激活受体α(PPARα)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量13.5kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Pro61~Ala170 (Accession # Q07869) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
等电点-
过氧化物酶体增殖物激活受体α(PPARα)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Peroxisome Proliferator Activated Receptor Alpha (PPARa)
Organism: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
[ DESCRIPTION ]
Protein Names: Peroxisome Proliferator Activated
Receptor Alpha
Synonyms: PPARa, NR1C1, PPAR
Species: Human
Size: 100μg
Source: Escherichia coli-derived
Subcellular Location: Nucleus.
过氧化物酶体增殖物激活受体α(PPARα)重组蛋白[ PROPERTIES ]
Residues: Pro61~Ala170 (Accession # Q07869), with
N-terminal His-Tag.
Grade & Purity: >95%, 15kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose, 0.01% sarcosyl.
Endotoxin Level: <1.0 EU per 1μg (determined by
the LAL method).
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Molecular Mass: 13.5kDa
Predicted isoelectric point: 8.9
Human PPARa
过氧化物酶体增殖物激活受体α(PPARα)重组蛋白[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- PGSDGSVITD TLSPASSPSS VTYPVVPGSV DESPSGALNI
ECRICGDKAS GYHYGVHACE GCKGFFRRTI RLKLVYDKCD RSCKIQKKNR NKCQYCRFHK
CLSVGMSHNA
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