红细胞生成素受体(EPOR)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位Cell membrane;Single-pass type I membrane protei
预测分子量55.8kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Arg276~Ala496 (Accession # P19235) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
红细胞生成素受体(EPOR)重组蛋白等电点5.0
应用SDS-PAGE; WB; ELISA; IP
Recombinant Erythropoietin Receptor (EPOR)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Arg276~Ala496 (Accession # P19235),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Cell membrane;
Single-pass type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.05%
sarcosyl and preservative.
Predicted isoelectric point: 5.0
Predicted Molecular Mass: 55.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
红细胞生成素受体(EPOR)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- RALKQ KIWPGIPSPE
SE F E G L F T T H KG N F Q LW LY Q ND G C LWW SP C TP F TE DP PA S LE V LS E R CWG
TMQAVEPGTD DEGPLLEPVG SEHAQDTYLV LDKWLLPRNP PSEDLPGPGG SVDIVAMDEG
SEASSCSSAL ASKPSPEGAS AASFEYTILD PSSQLLRPWT LCPELPPTPP HLKYLYLVVS
DSGISTDYSS GDSQGAQGGL SDGPYSNPYE NSLIPA
红细胞生成素受体(EPOR)重组蛋白[ REFERENCES ]
1. Winkelmann J.C., et al. (1990) Blood 76:24-30. 2. Jones S.S., et al. (1990) Blood 76:31-35. 3. Ehrenman K., St John T. (1991) Exp. Hematol. 19:973-977. 4. Kirito K., et al. (2002) Blood 99:102-110.
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成立日期
2015-05-29
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100
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