无翅型MMTV整合位点家族成员10A(WNT10A)重组蛋白

无翅型MMTV整合位点家族成员10A(WNT10A)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位n/a

预测分子量53.0kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Ser108~Pro343 (Accession # Q9GZT5) with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 95%

无翅型MMTV整合位点家族成员10A(WNT10A)重组蛋白等电点-

Wingless Wingless Wingless Wingless Type MMTV Integration Integration Integration Integration Site Family, Family, Family, Family, Member 10A (WNT10A) (WNT10A) (WNT10A) (WNT10A)

Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

6th Edition (Revised in March, 2013)

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Ser108~Pro343 (Accession # Q9GZT5),

with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted, extracellular

space, extracellular matrix. Purity: Purity: Purity: Purity: >95%

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 9.1

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 53.0kDa

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

无翅型MMTV整合位点家族成员10A(WNT10A)重组蛋白[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its

sequence is listed below.

MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL

DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD

VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD

GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ

GPLGSEF- SSL ETRNKIPYES PIFSRGFRES AFAYAIAAAG VVHAVSNACA LGKLKACGCD

AS R R G DEEA F RRK L H R L Q L D AL Q R G KG LS H GVPE HPA L P T ASP G L Q DSW E

WGGCSPDMGF GERFSKDF LD SREPHRDIHA RMRL HNNRVG RQAVMENMRR

KCKCHGTSGS CQLKTCWQVT PEFRTVGALL RSRFHRATLI RPHNRNGGQL EPGPAGAPSP

APGAPGPRRR ASPADLVYFE KSP

无翅型MMTV整合位点家族成员10A(WNT10A)重组蛋白[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. van den Boogaard M.J., et al. (2012) J. Med. Genet. 49:327-331.

2. Galbraith R.L., et al. (2011) Nutr Cancer 63:558-564.

3. Wedgeworth E.K., et al. (2011) Acta Derm. Venereol. 91:346-347.

4. Castori M., et al. (2011) Clin. Genet. 79:92-95.