卵巢肿瘤蛋白1(OTUB1)重组蛋白
物种Homo sapiens (Human,人)
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量32.5kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Met1~Lys271 (Accession # Q96FW1) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度>97%
等电点-
卵巢肿瘤蛋白1(OTUB1)重组蛋白应用SDS-PAGE; WB; ELISA; IP.
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
1th Edition (Revised in February, 2012)
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Otubain 1
Gene Names: OTUB1
Size: 100μg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: Hydrolase that can remove conjugated ubiquitin from proteins and plays an
important regulatory role at the level of protein turnover by preventing degradation.
Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered
unresponsive to antigen rechallenge and no longer respond to their cognate antigen.
Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy.
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: 卵巢肿瘤蛋白1(OTUB1)重组蛋白Location: Cytoplasm
Tissue Specificity: Specificity: Specificity: Specificity: Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid
tissues such as tonsils, lymph nodes, spleen and peripheral blood mononuclear cells.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Met1~Lys271 (Accession # Q96FW1), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 32.53 kDa as determined by SDS-PAGE reducing conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 32.53 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term
storage. Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGS-MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE
YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGF
TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV
KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP EGSEPKVYLL YRPGHYDILY K
卵巢肿瘤蛋白1(OTUB1)重组蛋白[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Borodovsky A., et al. (2002) Chem. Biol. 9:1149-1159. 2. Balakirev M.Y., et al. (2003) EMBO Rep. 4:517-522. 3. Stanisic V., et al. (2009) Borodovsky A., et al. J. Biol. Chem. 284:16135-16145.
4. Soares L., et al. (2004) Nat. Immunol. 5:45-54. 5. Edelmann M.J., et al. (2009) Biochem. J. 418:379-390.
6. Wang T., et al. (2009) J. Mol. Biol. 386:1011-1023.
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