人蔗糖酶异麦芽糖酶检测试剂盒

人蔗糖酶异麦芽糖酶检测试剂盒都是一个试剂盒对应检测一个指标。不存在可以用一个试剂盒同时检测出多个指标。进口原装的有96T的，进口分装的有48T 和 96T的 ，这个得根据老师自己的需要来选择试剂盒的大小和规格，一般96T的可以测85个样本，48T的可以做37次实验，ELISA试剂盒还有11个孔是做标准曲线分析实验数据用的。试剂盒选购好了之后就可以开始测了，ELISA实验必要的一种仪器-----酶标仪！

人蔗糖酶异麦芽糖酶检测试剂盒问：

1 请问为什么有的试剂盒要求反应过夜，而有的则只需要1h？反应时间不同是由什么决定的呢？

2 稀释的抗体如何保持其稳定性？

3 显色体系（加H2O2后）的稳定性如何保持？

4 如何克服批间差异较大的缺陷？

人蔗糖酶异麦芽糖酶检测试剂盒答：

确定的反应工艺有关，一般37度1小时足够了。

稀释道抗体的话，一般都会在稀释液中增加一些无关蛋白，使稀释液有一定的蛋白浓度，也可以添加一些蛋白稳定剂，但关键还是看抗体本身的稳定性怎样。

一般目前国内的显色系统都是将TMB（OPD）和过氧化氢放开保存的。

批间差异大的问题，第一就是要原料质量过关，原料的批和批之间差异不能太大，还有就是用于生产的工艺要简单，人蔗糖酶异麦芽糖酶检测试剂盒重复性好，这样可以尽量减少批间差异。

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