人胱天蛋白酶9(CASP9)ELISA试剂盒

人胱天蛋白酶9(CASP9)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人胱天蛋白酶9(CASP9)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人胱天蛋白酶9(CASP9)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人胱天蛋白酶9(CASP9)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人胱天蛋白酶9(CASP9)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H2298c 人对氧磷酶1(PON1)ELISA试剂盒 Human PON1 (Paraoxonase 1) ELISA Kit

XY-EL-H2299c 人中性粒细胞防御素1-3(HNP1-3)ELISA试剂盒 Human HNP1-3 (Neutrophil Peptide 1-3) ELISA Kit

XY-EL-H2300c 人中性粒细胞激活蛋白3(NAP3)ELISA试剂盒 Human NAP3 (Neutrophil Activating Protein 3) ELISA Kit 

XY-EL-H2301c 人中性粒细胞碱性磷酸酶(NAP)ELISA试剂盒 Human NAP (Neutrophil Alkaline Phosphatase) ELISA Kit

XY-EL-H2302c 人抑丝蛋白1(PFN1)ELISA试剂盒 Human PFN1 (Profilin 1) ELISA Kit

XY-EL-H2303c 人解整合素金属蛋白酶22(ADAM22)ELISA试剂盒 Human ADAM22 (A Disintegrin And Metalloprotease 22) ELISA Kit 

XY-EL-H2304c 人肿瘤蛋白p63(TP63)ELISA试剂盒 Human TP63 (Tumor Protein p63) ELISA Kit 

XY-EL-H2305c 人肿瘤坏死因子α转化酶(TACE)ELISA试剂盒 Human TACE (TNF α Converting Enzyme) ELISA Kit

XY-EL-H2306c 人肿瘤坏死因子β(TNF-β)ELISA试剂盒 Human TNF-β (Tumor Necrosis Factor Beta) ELISA Kit 

XY-EL-H2307c 人肿瘤坏死因子配体相关分子-1A(TL1A)ELISA试剂盒 Human TL1A (Tumor Necrosis Factor Related Ligand 1A) ELISA Kit

XY-EL-H2308c 人T-复合蛋白1θ亚基(CCT8)ELISA试剂盒 Human CCT8 (T-complex protein 1 subunit theta) ELISA kit

XY-EL-H2309c 人T-复合蛋白1亚基θ样蛋白1(CCT8L1)ELISA试剂盒 Human CCT8L1 (T-complex 1 protein subunit theta-like 1) ELISA kit

XY-EL-H2310c 人T-复合蛋白1亚基ε(CCT5)ELISA试剂盒 Human CCT5 (T-complex 1 protein subunit epsilon) ELISA kit

XY-EL-H2311c 人T-复合蛋白1亚基ζ(CCT6A)ELISA试剂盒 Human CCT6A (T-complex 1 protein subunit zeta) ELISA kit

XY-EL-H2312c 人肿瘤坏死因子相关弱凋亡诱导因子(TWEAK)ELISA试剂盒 Human TWEAK (Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit

XY-EL-H2313c 人肿瘤特异生长因子/肿瘤相关因子(TSGF)ELISA试剂盒 Human TSGF (Tumor Specific Growth Facter/Tumor Supplied Group of Factor) ELISA Kit

XY-EL-H2315c 人肿瘤特异性移植抗原(TSTA)ELISA试剂盒 Human TSTA (Tumor Specific Transplantation Antigen) ELISA Kit

XY-EL-H2316c 人肿瘤相关抗原(TAA)ELISA试剂盒 Human TAA (Tumor Associated Antigen) ELISA Kit

XY-EL-H2318c 人重酒石酸去甲肾上腺素(NEB)ELISA试剂盒 Human NEB (Noradrenaline Bitartas) ELISA Kit

XY-EL-H2319c 人重组激活基因1(RAG-1)ELISA试剂盒 Human RAG-1 (Recombination Activating Gene 1) ELISA Kit

XY-EL-H2320c 人重组激活基因2(RAG-2)ELISA试剂盒 Human RAG-2 (Recombination Activating Gene 2) ELISA Kit

XY-EL-H2321c 人周期素依赖性激酶1(CDK1)ELISA试剂盒   Human CDK1 (Cyclin Dependent Kinase 1) ELISA Kit

XY-EL-H2322c 人周期素依赖性激酶2(CDK2)ELISA试剂盒 Human CDK2 (Cyclin Dependent Kinase 2) ELISA Kit

XY-EL-H2323c 人周期素依赖性激酶4(CDK4)ELISA试剂盒   Human CDK4 (Cyclin Dependent Kinase 4) ELISA Kit

XY-EL-H2324c 人周期素依赖性激酶5(CDK5)ELISA试剂盒 Human CDK5 (Cyclin Dependent Kinase 5) ELISA Kit

XY-EL-H2325c 人周期素依赖性激酶7(CDK7)ELISA试剂盒 Human CDK7 (Cyclin Dependent Kinase 7) ELISA Kit

XY-EL-H2326c 人周期素依赖性激酶9(CDK-9)ELISA试剂盒 Human CDK9 (Cyclin Dependent Kinase 9) ELISA Kit

XY-EL-H2327c 人周期素依赖性激酶抑制因子2A(CDKN2A)ELISA试剂盒 Human CDKN2A (Cyclin Dependent Kinase Inhibitor 2A) ELISA Kit

XY-EL-H2328c 人轴突生长诱向因子1(Ntn1)ELISA试剂盒 Human Ntn1 (Netrin 1) ELISA Kit

XY-EL-H2329c 人轴突生长诱向因子4(Ntn4)ELISA试剂盒 Human Ntn4 (Netrin 4) ELISA Kit

XY-EL-H2330c 人主要穹窿蛋白(MVP)ELISA试剂盒 Human MVP (Major Vault Protein) ELISA Kit

XY-EL-H2331c 人I类主要组织相容性复合体C(MHCC/HLA-C)ELISA试剂盒 Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) ELISA Kit 

XY-EL-H2332c 人I类主要组织相容性复合体E(MHCE/HLA-E)ELISA试剂盒 Human MHCE/HLA-E (Major Histocompatibility Complex Class I E) ELISA Kit 

XY-EL-H2333c 人α-甲基酰基辅酶A消旋酶(AMACR)ELISA试剂盒 Human AMACR(Alpha-methylacyl-CoA racemase) ELISA kit

XY-EL-H2334c 人细胞周期素A1(CCNA1) ELISA试剂盒 Human CCNA1(Cyclin-A1) ELISA kit