磷酸化粘着斑激酶抗体

英文名称    phospho-FAK(Tyr577)    

中文名称    磷酸化粘着斑激酶抗体    

别    名    FAK (phospho Y577); p-FAK (phospho Y577); FADK 1; FADK; FAK 1; FAK related non kinase polypeptide; FAK1; Focal adhesion kinase 1; FRNK; pp125FAK; Protein tyrosine kinase 2; Protein Tyrosine Kinase Cytoplasmic; PTK 2; FAK1_HUMAN; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK.    

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  神经生物学  信号转导  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化粘着斑激酶抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    116kDa    

细胞定位    细胞核 细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human FAK around the phosphorylation site of Tyr577    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化粘着斑激酶抗体产品介绍    background:

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions.

Function:
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Subunit:
Interacts (via first Pro-rich region) with CAS family members (via SH3 domain), including BCAR1, BCAR3, CASS4 and NEDD9. Interacts with GIT1. Interacts with SORBS1. Interacts with RGNEF. Interacts with SHB. Interacts with PXN and TLN1. Interacts with STAT1. Interacts with DCC. Interacts with WASL. Interacts with ARHGEF7. Interacts with GRB2 and GRB7 (By similarity). Component of a complex that contains at least FER, CTTN and PTK2/FAK1. Interacts with BMX. Interacts with TGFB1I1. Interacts with STEAP4. Interacts with ZFYVE21. Interacts with ESR1. Interacts with PIK3R1 or PIK3R2. Interacts with SRC, FGR, FLT4 and RET. Interacts with EPHA2 in resting cells; activation of EPHA2 recruits PTPN11, leading to dephosphorylation of PTK2/FAK1 and dissociation of the complex. Interacts with EPHA1 (kinase activity-dependent). Interacts with CD4; this interaction requires the presence of HIV-1 gp120. Interacts with PIAS1. Interacts with ARHGAP26 and SHC1. Interacts with RB1CC1; this inhibits PTK2/FAK1 activity and activation of downstream signaling pathways. Interacts with P53/TP53 and MDM2. Interacts with LPXN (via LD motif 3).

Subcellular Location:
Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Nucleus. Note=Constituent of focal adhesions. Detected at microtubules.

Tissue Specificity:
Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.

Post-translational modifications:
Phosphorylated on tyrosine residues upon activation, e.g. upon integrin signaling. Tyr-397 is the major autophosphorylation site, but other kinases can also phosphorylate this residue. Phosphorylation at Tyr-397 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-576, Tyr-577 and at additional tyrosine residues. FGR promotes phosphorylation at Tyr-397 and Tyr-576. FER promotes phosphorylation at Tyr-577, Tyr-861 and Tyr-925, even when cells are not adherent. Tyr-397, Tyr-576 and Ser-722 are phosphorylated only when cells are adherent. Phosphorylation at Tyr-397 is important for interaction with BMX, PIK3R1 and SHC1. Phosphorylation at Tyr-925 is important for interaction with GRB2. Dephosphorylated by PTPN11; PTPN11 is recruited to PTK2 via EPHA2 (tyrosine phosphorylated). Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly; this dephosphorylation could be catalyzed by PTPN11 and regulated by ZFYVE21. 
Sumoylated; this enhances autophosphorylation.

DISEASE:
Note=Aberrant PTK2/FAK1 expression may play a role in cancer cell proliferation, migration and invasion, in tumor formation and metastasis. PTK2/FAK1 overexpression is seen in many types of cancer.

Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. 
FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.    

磷酸化粘着斑激酶抗体

hz-1090 BAFF-R(Human B cell activation factorr from the tumor necrosis factor family receptor) ELISA Kit  人B细胞活化因子受体

hz-1090 HSF1(Mouse Heat Shock Factor 1) ELISA Kit  小鼠热休克因子1

hz-1091 VEGFR-3/Flt-4(Human Vascuoar endothelial cell growth factor receptor 2) ELISA kit  人血管内皮细胞生长因子受体3

hz-1091 IAA ELISA Kit   大鼠胰岛素自身抗体

hz-1091 PF3(Mouse platelet factor 3) ELISA Kit  小鼠血小板因子3

hz-1092 gp210 ELISA Kit  大鼠抗核膜糖蛋白210抗体

hz-1092 IL-2R(Mouse Interleukin-2 receptor) ELISA kit  小鼠白介素2受体

hz-1092 VEGFR-1/Flt1(Human Vascuoar endothelial cell growth factor receptor 1) ELISA kit  人血管内皮细胞生长因子受体1

hz-1093 sTLR6 ELISA Kit  大鼠可溶性Toll样受体6

hz-1093 VEGF-D(Human Vascular Endothelial cell Growth Factor D) ELISA KIT  人血管内皮细胞生长因子D

hz-1093 ADM(Mouse adrenomedullin) ELISA Kit  小鼠肾上腺髓质素

hz-1094 VEGF-C(Human Vascular Endothelial cell Growth Factor C) ELISA KIT  人血管内皮细胞生长因子C

hz-1094 sTLR2 ELISA Kit  大鼠可溶性Toll样受体2

hz-1094 sLR(Mouse Leptin Soluble Receptor) ELISA Kit  小鼠可溶性瘦素受体

hz-1095 VEGF-B(Human Vascular Endothelial cell Growth Factor B) ELISA KIT  人血管内皮细胞生长因子B

hz-1095 PGF2 Alpha ELISA Kit  大鼠前列腺素F2α

hz-1095 Beta-EPR(Mouse Beta-Endorphin receptor) ELISA Kit  小鼠β内啡肽受体

hz-1096 VEGF(Human Vascular Endothelial cell Growth Factor) ELISA KIT  人血管内皮细胞生长因子

hz-1096 LTE4 ELISA Kit  大鼠白三烯E4

hz-1096 HL(Mouse hepatic lipase) ELISA Kit  小鼠肝脂酶

hz-1097 UK ELISA Kit  大鼠尿激酶

hz-1097 VCAM-1/CD106(Human Vascuolar cell adhesion molecule 1) ELISA kit  人血管内皮细胞粘附分子1

hz-1097 HBcAb(Mouse hepatitis B virus core antibody) ELISA Kit  小鼠乙型肝炎核心抗体

hz-1098 HBeAb(Mouse hepatitis B virus e antibody) ELISA Kit  小鼠乙型肝炎e抗体

hz-1098 sTRAIL(Human soluble tumor necrosis factor-related apoptosis inducing ligand) ELISA KIT  人可溶性肿瘤坏死因子相关凋亡诱导配体

hz-1098 F XII ELISA Kit  大鼠凝血因子Ⅻ

hz-1099 F X III ELISA Kit  大鼠凝血因子ⅩⅢ

hz-1099 TRAIL-R4(Human tumor necrosis factor-related apoptosis-inducing ligand 4) ELISA Kit  人肿瘤坏死因子相关凋亡诱导配体4

hz-1099 HBeAg(Mouse hepatitis B virus e antigen) ELISA Kit  小鼠乙型肝炎e抗原

hz-1100 TRAIL-R3(Human tumor necrosis factor-related apoptosis-inducing ligand 3) ELISA Kit  人肿瘤坏死因子相关凋亡诱导配体3

hz-1100 F III ELISA Kit  大鼠凝血因子Ⅲ

hz-1100 HBsAb(Mouse hepatitis B virus surface antibody) ELISA Kit  小鼠乙型肝炎表面抗体