磷酸化HER2受体抗体

英文名称    Phospho-HER2 (Tyr1139)    

中文名称    磷酸化HER2受体抗体    

别    名    ErbB 2 (phospho Y1139); p-ErbB 2 (phospho Y1139); ErbB 2 (phospho T1139); p-ErbB 2 (phospho T1139); CerbB2; c erb B2; C erbB 2; C-erbB2; CD340; CD340 antigen; Cerb B2/neu protein; Erb B2; erbb2; HER 2; HER 2/neu; HER2; Her2/neu; Herstatin; MLN 19; MLN19; NEU; NEU Proto Oncogene; Neuro Glioblastoma Derived Oncogene Homolog; Neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185 ErbB2; p185erbB2; Receptor Protein Tyrosine Kinase ErbB2 Precursor; Receptor tyrosine protein kinase erbB 2; TKR1.    

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  生长因子和激素  细胞膜受体  细胞类型标志物      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 磷酸化HER2受体抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    138kDa    

细胞定位    细胞核 细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human HER2 around the phosphorylation site of Tyr1139 [PE(p-Y)VN]    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化HER2受体抗体产品介绍    background:

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008].

Function:
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Subcellular Location:
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.

Tissue Specificity:
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Post-translational modifications:
Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Signaling via SEMA4C promotes phosphorylation at Tyr-1248.

DISEASE:
Defects in ERBB2 are a cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. A cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body.
Defects in ERBB2 are involved in the development of glioma (GLM) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas.
Defects in ERBB2 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. Defects in ERBB2 may be a cause of lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
Defects in ERBB2 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
Note=Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.    

磷酸化HER2受体抗体

hz-0294D-hzy7 Donkey Anti-Goat IgG/Cy7  Cy7标记的驴抗羊IgG

hz-0294D-PE Donkey Anti-Goat IgG/PE  PE标记的驴抗羊IgG

hz-0294D-PE-hzy3 Donkey Anti-Goat IgG/PE-Cy3  PE-Cy3标记的驴抗羊IgG

hz-0294D-PE-hzY5 Donkey Anti-Goat IgG/PE-CY5  PE-CY5标记的驴抗羊IgG

hz-0294D-APhz Donkey Anti-Goat IgG/APC  APC标记的驴抗羊IgG

hz-0294D-AF350 Donkey Anti-Goat IgG/Alexa Fluor 350  Alexa Fluor 350标记的驴抗羊IgG

hz-0294D-AF488 Donkey Anti-Goat IgG/Alexa Fluor 488  Alexa Fluor 488标记的驴抗羊IgG

hz-0294D-RBIThz Donkey Anti-Goat IgG/RBITC  罗丹明标记的驴抗羊IgG

hz-0294D-Bio Donkey Anti-Goat IgG/Bio  生物素标记的驴抗羊IgG

hz-0358D-Gold Donkey Anti-Guinea pig IgG/Gold  胶体金标记的驴抗豚鼠IgG

hz-0358D-HRP Donkey Anti-Guinea pig IgG/HRP  辣根过氧化物酶标记的驴抗豚鼠IgG

hz-0358D-Bio Donkey Anti-Guinea pig IgG/Bio  生物素标记的驴抗豚鼠IgG

hz-0358D-AP Donkey Anti-Guinea pig IgG/AP  碱性磷酸酶（AP）标记的驴抗豚鼠IgG

hz-0358D-hzy3 Donkey Anti-Guinea pig IgG/Cy3  Cy3标记的驴抗豚鼠IgG

hz-0358D-hzy5 Donkey Anti-Guinea pig IgG/Cy5  Cy5标记的驴抗豚鼠IgG

hz-0358D-hzy5.5 Donkey Anti-Guinea pig IgG/Cy5.5  Cy5.5标记的驴抗豚鼠IgG

hz-0358D-hzy7 Donkey Anti-Guinea pig IgG/Cy7  Cy7标记的驴抗豚鼠IgG

hz-0358D-PE Donkey Anti-Guinea pig IgG/PE  PE标记的驴抗豚鼠IgG

hz-0358D-PE-hzy3 Donkey Anti-Guinea pig IgG/PE-Cy3  PE-Cy3标记的驴抗豚鼠IgG

hz-0358D-PE-hzY5 Donkey Anti-Guinea pig IgG/PE-CY5  PE-CY5标记的驴抗豚鼠IgG

hz-0358D-APhz Donkey Anti-Guinea pig IgG/APC  APC标记的驴抗豚鼠IgG

hz-0358D-AF350 Donkey Anti-Guinea pig IgG/Alexa Fluor 350  Alexa Fluor 350标记的驴抗豚鼠IgG

hz-0358D-AF488 Donkey Anti-Guinea pig IgG/Alexa Fluor 488  Alexa Fluor 488标记的驴抗豚鼠IgG

hz-0358D-AF555 Donkey Anti-Guinea pig IgG/Alexa Fluor 555  Alexa Fluor 555标记的驴抗豚鼠IgG

hz-0358D-AF647 Donkey Anti-Guinea pig IgG/Alexa Fluor 647  Alexa Fluor 647标记的驴抗豚鼠IgG

hz-0358D-PE-hzy5.5 Donkey Anti-Guinea pig IgG/PE-Cy5.5  PE-Cy5.5标记的驴抗豚鼠IgG

hz-0358D-PE-hzy7 Donkey Anti-Guinea pig IgG/PE-Cy7  PE-Cy7标记的驴抗豚鼠IgG

hz-0358D-FIThz Donkey Anti-Guinea pig IgG/FITC  FITC标记的驴抗豚鼠IgG

hz-0358D-RBIThz Donkey Anti-Guinea pig IgG/RBITC  罗丹明标记的驴抗豚鼠IgG

hzm-0297M-RBIThz Mouse Anti-human IgG(3D3)/RBITC  罗丹明标记的鼠抗人IgG单克隆抗体

hzm-0297M-FIThz Mouse Anti-human IgG(3D3)/FITC  FITC标记的鼠抗人IgG单克隆抗体

hzm-0297M-Gold Mouse Anti-human IgG(3D3)/Gold  胶体金标记的鼠抗人IgG单克隆抗体

hzm-0297M-hzy3 Mouse Anti-human IgG(3D3)/Cy3  Cy3标记的鼠抗人IgG单克隆抗体