磷酸化DNA依赖性蛋白激酶抗体

英文名称    phospho-DNA PKcs (Ser2612)    

中文名称    磷酸化DNA依赖性蛋白激酶抗体    

别    名    PRKDC (phospho S2612); DNA PKcs (phospho S2612); p-DNA PKcs (phospho S2612); DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNA PKcs; DNPK1; p460; PRKDC; HYRC; HYRC1; Variant; DNAPK; DNAPK catalytic subunit; p350; PRKDC; Protein Kinase DNA Activated Catalytic Polypeptide; XRCC 7; XRCC7; PRKDC_HUMAN.    

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  免疫学  信号转导  转录调节因子  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Cow,     

产品应用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 磷酸化DNA依赖性蛋白激酶抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    454kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human PRKDC around the phosphorylation site of Ser2612    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化DNA依赖性蛋白激酶抗体产品介绍    background:

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Function:
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.

Subunit:
DNA-PK is a heterotrimer of PRKDC and the Ku p70-p86 (XRCC6-XRCC5) dimer. Formation of this complex may be promoted by interaction with ILF3. Associates with the DNA-bound Ku heterodimer, but it can also bind to and be activated by free DNA. Interacts with DNA-PKcs-interacting protein (KIP) with the region upstream the kinase domain. PRKDC alone also interacts with and phosphorylates DCLRE1C, thereby activating the latent endonuclease activity of this protein. Interacts with C1D. Interacts with TTI1 and TELO2.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair. 
S-nitrosylated by GAPDH.    

磷酸化DNA依赖性蛋白激酶抗体

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hz-10031R Kappa-casein/CSN3  κ-酪蛋白抗体

hz-10032R Beta-casein  β-酪蛋白抗体

hz-10033R Alpha s1 Casein  α-s1酪蛋白抗体

hz-10034R Alpha s2 Casein  α-s2酪蛋白抗体

hz-4233R DRAM  DNA损伤自噬调整蛋白抗体

hz-0634R AQP4  水通道蛋白-4抗体

hz-1554R AQP5  水通道蛋白5抗体

hz-2506R AQP7  水通道蛋白-7抗体

hz-4234R ABAT  γ氨基丁酸转氨酶抗体

hz-2060R AQP9  水通道蛋白-9抗体

hz-0118R Androgen receptor  雄激素受体抗体

hz-5178R Chondroitin Sulfate  硫酸软骨素抗体

hz-0943R phospho-Androgen Receptor(Ser580)  磷酸化雄激素受体抗体

hz-3004R phospho-Androgen Receptor(Ser595)  磷酸化雄激素受体抗体

hz-2903R ARHI  抑癌基因ras同源家族1抗体

hz-5191R phospho-Androgen Receptor (Ser212)  磷酸化雄激素受体抗体

hz-3847R Amphiregulin  双调蛋白（结肠直肠细胞源性生长因子）抗体

hz-2405R AKR1B1  醛糖还原酶抗体

hz-3751R ARA24  雄激素受体相关蛋白24抗体

hz-5192R phospho-Androgen Receptor(Ser660)  磷酸化雄激素受体抗体

hz-0385R ARC/Arg3.1  ARC抗体

hz-1145R ARF6  ADP核糖基化因子6抗体

hz-1477R Arfaptin 1  ADP核糖基化因子结合蛋白1抗体

hz-4230R mSin3A  同源转录调节蛋白Sin3A抗体

hz-1442R Arfaptin 2  ADP核糖基化因子结合蛋白2抗体

hz-3006R phospho-arfaptin 2(Ser260)  磷酸化ADP核糖基化因子结合蛋白2抗体

hz-4202R Arhgef2/GEF H1  Rho鸟苷酸交换因子2抗体

hz-2201R phospho-Arhgef2(Ser810)  磷酸化Rho鸟苷酸交换因子2抗体

hz-0114M Aromatase  芳香化酶抗体

hz-0114R Aromatase  芳香化酶/细胞色素P450/雌激素合成酶抗体

hz-3953R COX1/MTCO1  细胞色素c氧化酶1抗体