磷酸化DNA甲基转移酶1抗体

英文名称    phospho-Dnmt1(Ser154)    

中文名称    磷酸化DNA甲基转移酶1抗体    

别    名    Dnmt1(phospho S154); Dnmt1 (phospho Ser154); p-Dnmt1 (Ser154); AIM; CXXC finger protein 9; CXXC-type zinc finger protein 9; CXXC9; DNA (cytosine 5 ) methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase M.HsaI.; DNA MTase; DNA MTase HsaI; DNMT 1; DNMT; Dnmt1; Dnmt1; DNMT1_HUMAN; Dnmt1o; FLJ16293; HSN1E; M.HsaI; MCMT; Met1; MGC104992; mMmul; MommeD2.    

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  免疫学  细胞凋亡      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Dog, Pig, Cow, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500  磷酸化DNA甲基转移酶1抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    178kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human Dnmt1 around the phosphorylation site of Ser153    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化DNA甲基转移酶1抗体产品介绍    background:

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008].

Function:
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

Subunit:
Binds to CSNK1D (By similarity). Homodimer. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Component of complexes containing SUV39H1. Interacts with DNMT3A and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with UBC9 and BAZ2A/TIP5.

Subcellular Location:
Nucleus.

Tissue Specificity:
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.

Post-translational modifications:
Sumoylated; sumoylation increases activity. 
Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity. 
Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability. 
Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.

DISEASE:
Defects in DNMT1 are the cause of hereditary sensory neuropathy type 1E (HSN1E) [MIM:614116]. A neurodegenerative disorder characterized by adult onset of progressive peripheral sensory loss associated with progressive hearing impairment and early-onset dementia.    

磷酸化DNA甲基转移酶1抗体

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hz-9706R JWA  JWA蛋白抗体

hz-9707R Kazrin  Kazrin蛋白抗体

hz-9708R LCE1A  晚期包膜蛋白1抗体

hz-9709R LCE2B  晚期包膜蛋白2B抗体

hz-9710R NOL1  增殖相关核仁抗原抗体

hz-9711R LIPK  脂肪酶家庭成员K抗体

hz-9712R NHEDC2  线粒体钠/氢交换蛋白质2抗体

hz-9713R NOC3L/FAD24  脂肪细胞分化因子24

hz-9715R Sox9a  转录因子sox9a抗体

hz-9716R KBP  KBP蛋白抗体

hz-9717R Nidogen 2  巢蛋白NID2抗体

hz-9718R Nanos3  骨巢蛋白抗体

hz-9719R PDE4A8  磷酸二酯酶4A8抗体

hz-9720R C10orf27/SPATIAL  第10号染色体开放阅读框27抗体

hz-9721R FEZ1  脑胚胎锌指样蛋白1抗体

hz-9723R FAM29A/HAUS6  FAM29蛋白抗体

hz-9724R E4F1  E4F转录因子1抗体

hz-9725R CHPT1  甘油二酯胆碱磷酸转移酶抗体

hz-9727R BIN2/BRAP1  乳腺癌相关蛋白1抗体

hz-9728R B4GALT7/GlcNAc beta  半乳糖转移酶7亚基β1,4抗体

hz-9729R FAM96B  FAM96B蛋白抗体

hz-9730R FAM82B  微管相关蛋白FAM82B抗体

hz-9731R hzL3  微管相关蛋白样蛋白3抗体

hz-1489R Apollon/BIRC6  Apollon凋亡抑制蛋白抗体

hz-9732R ZWILCH  着丝粒ZWILCH蛋白抗体

hz-9733R UFD1L  泛素融合降解样蛋白1抗体

hz-9734R TSSK6/CT72  睾丸特异丝氨酸/苏氨酸激酶6抗体

hz-9735R TSEN2  tRNA剪接内切酶2抗体

hz-9736R TPD52L1/D53  肿瘤蛋白D53抗体

hz-9737R EDRF1/C10orf137  红细胞分化相关因子1抗体

hz-9738R FASP1/C21orf45  MaFF相关蛋白抗体

hz-9739R CNTD  细胞周期蛋白N端结构域蛋白1抗体

hz-9740R CNTD2  细胞周期蛋白N端结构域蛋白2抗体

hz-9741R ANGEL1  ANGEL1蛋白抗体

hz-9742R ANGEL2  ANGEL2蛋白抗体