骨钙素(OC)检测试剂盒适用生物     Bos taurus; Bovine (Cattle，牛)    骨钙素(OC)检测试剂盒    检测范围     78.13-5000pg/mL     灵敏度     34pg/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     骨钙素(OC)检测试剂盒   规格     96T    ELISA Kit for Osteocalcin (OC)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Bos taurus; Bovine (Cattle)    骨钙素(OC)检测试剂盒    Sample type     Serum, plasma, tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     78.13-5000pg/mL The standard curve concentrations used for the ELISA’s were 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312.5pg/mL, 156.25pg/mL, 78.13pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 34pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC). No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Osteocalcin (OC) and the recovery rates were calculated by comparing the measured value to the expected amount of Osteocalcin (OC) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     96-104     101    EDTA plasma(n=5)     96-105     101    heparin plasma(n=5)     97-105     101    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteocalcin (OC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteocalcin (OC) were tested on 3 different plates, 骨钙素(OC)检测试剂盒    8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Osteocalcin (OC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     88-104%     84-93%     79-90%     88-99%    EDTA plasma(n=5)     84-101%     96-105%     78-92%     82-104%    heparin plasma(n=5)     89-97%     90-99%     79-101%     87-103%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle 骨钙素(OC)检测试剂盒    applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteocalcin (OC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin (OC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteocalcin (OC) in the 骨钙素(OC)检测试剂盒    samples is then determined by comparing the O.D. of the samples to the standard curve.

Toll样受体2(TLR2)检测试剂盒适用生物     Homo sapiens (Human，人)    Toll样受体2(TLR2)检测试剂盒    检测范围     0.313-20ng/mL     灵敏度     0.117ng/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     Toll样受体2(TLR2)检测试剂盒规格     96T    ELISA Kit for Toll Like Receptor 2 (TLR2)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Toll样受体2(TLR2)检测试剂盒   Sample type     Serum, plasma, tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.117ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 2 (TLR2). No significant cross-reactivity or Toll样受体2(TLR2)检测试剂盒interference between Toll Like Receptor 2 (TLR2) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Toll Like Receptor 2 (TLR2) and the recovery rates were calculated by comparing the measured value to the expected amount of Toll Like Receptor 2 (TLR2) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     84-93     90    EDTA plasma(n=5)     97-105     101    heparin plasma(n=5)     78-102     98    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed Toll样受体2(TLR2)检测试剂盒by testing samples spiked with appropriate concentration of Toll Like Receptor 2 (TLR2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     81-92%     99-105%     79-93%     78-101%    EDTA plasma(n=5)     97-104%     78-97%     96-103%     91-98%    heparin plasma(n=5)     96-103%     86-93%     79-91%     78-98%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air Toll样受体2(TLR2)检测试剂盒humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in thisToll样受体2(TLR2)检测试剂盒 kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 2 (TLR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 2 (TLR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 2 (TLR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution Toll样受体2(TLR2)检测试剂盒and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor 2 (TLR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

鳞状细胞癌抗原1(SCCA1)检测试剂盒适用生物     Homo sapiens (Human，人)    鳞状细胞癌抗原1(SCCA1)检测试剂盒   检测范围     78.13-5000pg/mL     灵敏度     31pg/mL    样本类型     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids    实验时长     4.5h     实验方法     双抗夹心法     鳞状细胞癌抗原1(SCCA1)检测试剂盒    规格     96T    ELISA Kit for Squamous Cell Carcinoma Antigen 1 (SCCA1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    鳞状细胞癌抗原1(SCCA1)检测试剂盒Sample type     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids    Format     96-well strip plate    Assay length     4.5 hours    Detection range     78.13-5000pg/mL The standard curve concentrations used for the ELISA’s were 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312.5pg/mL, 156.25pg/mL, 78.13pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 31pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Squamous Cell Carcinoma Antigen 1 (SCCA1). No significant cross-reactivity or interference between Squamous Cell Carcinoma Antigen 1 (SCCA1) and analogues was observed.鳞状细胞癌抗原1(SCCA1)检测试剂盒RecoveryMatrices listed below were spiked with certain level of recombinant Squamous Cell Carcinoma Antigen 1 (SCCA1) and the recovery rates were calculated by comparing the measured value to the expected amount of Squamous Cell Carcinoma Antigen 1 (SCCA1) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     80-102     94    EDTA plasma(n=5)     91-99     94    heparin plasma(n=5)     82-90     85    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Squamous Cell Carcinoma Antigen 1 (SCCA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Squamous Cell Carcinoma Antigen 1 (SCCA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Squamous Cell Carcinoma Antigen 1 (SCCA1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     86-94%     85-92%     91-101%     91-102%    EDTA plasma(n=5)     79-102%     88-101%     90-99%     92-101%    heparin plasma(n=5)     85-99%     79-95%     78-92%     80-98%    鳞状细胞癌抗原1(SCCA1)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 鳞状细胞癌抗原1(SCCA1)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Squamous Cell Carcinoma Antigen 1 (SCCA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Squamous Cell Carcinoma Antigen 1 (SCCA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Squamous Cell Carcinoma Antigen 1 (SCCA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Squamous Cell Carcinoma Antigen 1 (SCCA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

转醛缩酶(TALDO1)检测试剂盒适用生物     Homo sapiens (Human，人)    转醛缩酶(TALDO1)检测试剂盒    检测范围     0.156-10ng/mL     灵敏度     0.067ng/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     转醛缩酶(TALDO1)检测试剂盒    规格     96T    ELISA Kit for Transaldolase (TALDO1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    转醛缩酶(TALDO1)检测试剂盒   Sample type     Serum, plasma, tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.067ng/mL.    转醛缩酶(TALDO1)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Transaldolase (TALDO1). No significant cross-reactivity or interference between Transaldolase (TALDO1) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Transaldolase (TALDO1) and the recovery rates were calculated by comparing the measured value to the expected amount of Transaldolase (TALDO1) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     98-105     102    EDTA plasma(n=5)     85-93     89    heparin plasma(n=5)     80-105     88    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transaldolase (TALDO1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transaldolase (TALDO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transaldolase (TALDO1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     93-101%     87-95%     78-92%     87-105%    EDTA plasma(n=5)     88-95%     85-104%     87-101%     94-101%    heparin plasma(n=5)     96-103%     81-95%     79-98%     94-101%    转醛缩酶(TALDO1)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 转醛缩酶(TALDO1)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transaldolase (TALDO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transaldolase (TALDO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transaldolase (TALDO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transaldolase (TALDO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

乳酸脱氢酶A(LDHA)检测试剂盒适用生物     Homo sapiens (Human，人)    乳酸脱氢酶A(LDHA)检测试剂盒   检测范围     0.156-10ng/mL     灵敏度     0.062ng/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     乳酸脱氢酶A(LDHA)检测试剂盒    规格     96T    ELISA Kit for Lactate Dehydrogenase A (LDHA)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    乳酸脱氢酶A(LDHA)检测试剂盒  Sample type     Serum, plasma, tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.062ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Lactate Dehydrogenase A (LDHA). No significant cross-reactivity or interference between Lactate Dehydrogenase A (LDHA) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Lactate Dehydrogenase A (LDHA) and the recovery rates were calculated by comparing the measured value to the expected amount of Lactate Dehydrogenase A (LDHA) in samples. Matrix     Recovery range (%)     Average(%)    乳酸脱氢酶A(LDHA)检测试剂盒serum(n=5)     99-105     102    EDTA plasma(n=5)     80-102     94    heparin plasma(n=5)     86-103     95    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactate Dehydrogenase A (LDHA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactate Dehydrogenase A (LDHA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lactate Dehydrogenase A (LDHA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     93-103%     95-102%     93-101%     87-95%    EDTA plasma(n=5)     81-92%     89-97%     80-96%     87-94%    heparin plasma(n=5)     83-97%     91-102%     89-96%     92-101%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 乳酸脱氢酶A(LDHA)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase A (LDHA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactate Dehydrogenase A (LDHA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactate Dehydrogenase A (LDHA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactate Dehydrogenase A (LDHA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

田教授是北京解放军总医院生化科主任医师；教授；博士生导师；清华大学、南开大学兼职教授。田教授在会议上分享了血液生物标志的相关研究，同时该演讲视频已上传至行云学院供交流学习。MicroRNA（miRNA）是一类生物体内长度约22个左右核苷酸的小RNA，相关研究显示其广泛存在于多种体液中，且可稳定存在较长时间。miRNA在细胞内具有多种重要的调节作用，一个miRNA可以调节多个靶基因的表达，而几个miRNAs也可同时调节一个基因。恶性肿瘤的发生发展过程非常复杂，常常涉及多个基因的表达异常，因此miRNA复杂调节网络的紊乱与细胞增殖和凋亡过程的调节失控可能存在一定关联。为此我们利用芯片和 Solexa 测序技术筛选肿瘤相关miRNA，在血液中发现了一些与原发性肝癌、宫颈癌、肺癌等密切相关的miRNA，经Q-PCR方法临床验证，初步证明其具有一定辅助诊断价值，进一步研究可为恶性肿瘤的生物靶向治疗提供新的线索与靶点。

今天，由生物谷主办的2015肿瘤干细胞转化医学论坛在上海好望角大酒店隆重开幕。本次会议邀请来自知名高校，研究所及三甲医院的多位专家、教授作为演讲嘉宾。作为该领域的前言领头人，他们将围绕肿瘤干细胞研究进展与前沿技术、肿瘤干细胞的转化医学及应用、肿瘤干细胞研究面临的挑战与任务等热点议题展开精彩演讲和讨论。肿瘤干细胞(tumor stem cells，TSCs）被认为是恶性肿瘤难以治疗的关键因素之一。肿瘤干细胞也被称为肿瘤起始细胞（tumor initial cells）。肿瘤干细胞一方面可以自我复制保持"干性"，另一方面也可以分化成肿瘤细胞而促进肿瘤的生长。肿瘤干细胞理论，为人们重新认识肿瘤的起源和本质，肿瘤的临床诊断、治疗，以及新药研发提供了新的方向和视角。目前，在血液肿瘤和大部分实体瘤中均发现存在不同表型的肿瘤干细胞。但是，各种TSCs的来源、分离与鉴定、TSCs表型特征、TSCs生物学功能和特异性靶点，都还存在不同程度的争议；另外，肿瘤微环境与肿瘤干细胞之间的关系、肿瘤干细胞与免疫的关系也是争议的焦点。同时，针对肿瘤干细胞的靶向机制，也提出了多种新颖的治疗思路和策略。有理由相信，肿瘤干细胞可能是肿瘤领域未来10年最热的方向之一。此次研讨会为期两天，主要从肿瘤干细胞的基础研究，技术发展和转化医学三方面深入剖析，针对当前研究热点及问题开展交流，与会者将分享该领域最新的研究成果以及他们对肿瘤干细胞转化医学行业的认识与前景预测，为该领域的科学家搭建一个相互沟通交流的平台。本次会议的精彩演讲提炼将在生物谷app会议专区进行推出展示，有兴趣的谷友可以前往各手机应用商城下载生物谷app进行及时关注跟进。

多巴胺受体D1(DRD1)检测试剂盒适用生物     Homo sapiens (Human，人)    多巴胺受体D1(DRD1)检测试剂盒   检测范围     0.313-20ng/mL     灵敏度     0.109ng/mL    样本类型     Tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     多巴胺受体D1(DRD1)检测试剂盒规格     96T    ELISA Kit for Dopamine Receptor D1 (DRD1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     hzEB299Hu    Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.109ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Dopamine Receptor D1 (DRD1). No significant cross-reactivity or interference between Dopamine Receptor D1 (DRD1) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Receptor D1 (DRD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Receptor D1 (DRD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV多巴胺受体D1(DRD1)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 多巴胺受体D1(DRD1)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Receptor D1 (DRD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Receptor D1 (DRD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Receptor D1 (DRD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Receptor D1 (DRD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

肾上腺素能受体α1A(ADRα1A)检测试剂盒适用生物     Homo sapiens (Human，人)    肾上腺素能受体α1A(ADRα1A)检测试剂盒   检测范围     0.313-20ng/mL     灵敏度     0.114ng/mL    样本类型     Tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     肾上腺素能受体α1A(ADRα1A)检测试剂盒规格     96T    ELISA Kit for Adrenergic Receptor Alpha 1A (ADRa1A)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     hzEB298Hu    Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.114ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Alpha 1A (ADRa1A). No significant cross-reactivity or interference between Adrenergic Receptor Alpha 1A (ADRa1A) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenergic Receptor Alpha 1A (ADRa1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenergic Receptor Alpha 1A (ADRa1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV肾上腺素能受体α1A(ADRα1A)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 肾上腺素能受体α1A(ADRα1A)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Alpha 1A (ADRa1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Alpha 1A (ADRa1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Alpha 1A (ADRa1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenergic Receptor Alpha 1A (ADRa1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

钙结合蛋白(CR)检测试剂盒适用生物     Homo sapiens (Human，人)    钙结合蛋白(CR)检测试剂盒   检测范围     0.156-10ng/mL     灵敏度     0.059ng/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids    实验时长     4.5h     实验方法     双抗夹心法     钙结合蛋白(CR)检测试剂盒规格     96T    ELISA Kit for Calretinin (CR)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     hzEA687Hu    Sample type     Serum, plasma, tissue homogenates and other biological fluids    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.059ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Calretinin (CR). No significant cross-reactivity or interference between Calretinin (CR) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Calretinin (CR) and the recovery rates were calculated by comparing the measured value to the expected amount of Calretinin (CR) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     95-103     99    EDTA plasma(n=5)     99-105     102    heparin plasma(n=5)     80-94     90    钙结合蛋白(CR)检测试剂盒PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calretinin (CR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calretinin (CR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calretinin (CR) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     86-102%     86-102%     92-99%     94-102%    EDTA plasma(n=5)     79-91%     93-101%     81-105%     79-93%    heparin plasma(n=5)     94-102%     79-101%     83-90%     83-101%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    钙结合蛋白(CR)检测试剂盒Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 钙结合蛋白(CR)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calretinin (CR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calretinin (CR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calretinin (CR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calretinin (CR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.Gene/ItemProteinsAntibodiesAssay Kits        Package and ComponentsReagent PreparationResults demonstrationTypical Standard CurveCertificateDownloadFree use and GuaranteesFree trialIn case it is difficult to pre-evaluate experiment result because of specific test samples or other reasons, free trial can be offered.If the budget is really limited or other special situation, you can apply the free products.GuaranteesUnconditionally return policy within 72 hours.For any complaint, we promise to reply within one work day, resolve the problem within three work days. Implementing initial consultation system in order to provide satisfactory service for all the customers. Customers can unconditionally to request a charge back or refund.Benchmark pricePlease consult local dealer

神经降压素(NT)检测试剂盒适用生物     Homo sapiens (Human，人)    神经降压素(NT)检测试剂盒    检测范围     37.04-3000pg/mL     灵敏度     13.3pg/mL    样本类型     Serum, plasma and other biological fluids.    实验时长     2.5h     实验方法     竞争抑制法     神经降压素(NT)检测试剂盒规格     96T    ELISA Kit for Neurotensin (NT)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     CEB203Hu    Sample type     Serum, plasma and other biological fluids.    Format     96-well strip plate    Assay length     2.5 hours    Detection range     37.04-3000pg/mL The standard curve concentrations used for the ELISA’s were 3000pg/mL, 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 13.3pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Neurotensin (NT). No significant cross-reactivity or interference between Neurotensin (NT) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Neurotensin (NT) and the recovery rates were calculated by comparing the measured value to the expected amount of Neurotensin (NT) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     96-103     101    EDTA plasma(n=5)     95-102     98    heparin plasma(n=5)     78-103     91    神经降压素(NT)检测试剂盒PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotensin (NT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotensin (NT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurotensin (NT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     83-98%     87-97%     80-92%     83-90%    EDTA plasma(n=5)     87-96%     89-96%     92-104%     78-98%    heparin plasma(n=5)     94-105%     78-101%     80-91%     87-95%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    神经降压素(NT)检测试剂盒Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 50μL standard or sample to each well.    And then add 50μL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37oC;3. Aspirate and wash 3 times;4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;5. Aspirate and wash 5 times;6. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;7. Add 50μL Stop Solution. Read at 450 nm immediately.神经降压素(NT)检测试剂盒Test principleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurotensin (NT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurotensin (NT) and unlabeled Neurotensin (NT) (Standards or samples) with the pre-coated antibody specific to Neurotensin (NT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurotensin (NT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurotensin (NT) in the sample.

神经激肽B(NKB)检测试剂盒适用生物     Homo sapiens (Human，人)    神经激肽B(NKB)检测试剂盒   检测范围     9.88-800pg/mL     灵敏度     3.12pg/mL    样本类型     Serum, plasma and other biological fluids.    实验时长     2.5h     实验方法     竞争抑制法    神经激肽B(NKB)检测试剂盒规格     96T    ELISA Kit for Neurokinin B (NKB)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     CEB202Hu    Sample type     Serum, plasma and other biological fluids.    Format     96-well strip plate    神经激肽B(NKB)检测试剂盒Assay length     2.5 hours    Detection range     9.88-800pg/mL The standard curve concentrations used for the ELISA’s were 800pg/mL, 266.67pg/mL, 88.89pg/mL, 29.63pg/mL, 9.88pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 3.12pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Neurokinin B (NKB). No significant cross-reactivity or interference between Neurokinin B (NKB) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Neurokinin B (NKB) and the recovery rates were calculated by comparing the measured value to the expected amount of Neurokinin B (NKB) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     78-92     85    EDTA plasma(n=5)     79-98     95    heparin plasma(n=5)     78-90     87    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurokinin B (NKB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurokinin B (NKB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurokinin B (NKB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     91-99%     87-101%     87-98%     93-104%    EDTA plasma(n=5)     78-93%     79-99%     87-95%     93-101%    heparin plasma(n=5)     85-94%     94-102%     94-101%     95-104%    神经激肽B(NKB)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 50μL standard or sample to each well.    And then add 50μL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37oC;3. Aspirate and wash 3 times;4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;5. Aspirate and wash 5 times;6. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;7. Add 50μL Stop Solution. Read at 450 nm immediately.神经激肽B(NKB)检测试剂盒Test principleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurokinin B (NKB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurokinin B (NKB) and unlabeled Neurokinin B (NKB) (Standards or samples) with the pre-coated antibody specific to Neurokinin B (NKB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurokinin B (NKB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurokinin B (NKB) in the sample.

葡萄糖转运蛋白1(GLUT1)检测试剂盒适用生物     Homo sapiens (Human，人)    葡萄糖转运蛋白1(GLUT1)检测试剂盒   检测范围     0.313-20ng/mL     灵敏度     0.127ng/mL    样本类型     Tissue homogenates, cell lysates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法    葡萄糖转运蛋白1(GLUT1)检测试剂盒规格     96T    ELISA Kit for Glucose Transporter 1 (GLUT1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEB185Hu    Sample type     Tissue homogenates, cell lysates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.127ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucose Transporter 1 (GLUT1). No significant cross-reactivity or interference between Glucose Transporter 1 (GLUT1) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose Transporter 1 (GLUT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose Transporter 1 (GLUT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV葡萄糖转运蛋白1(GLUT1)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 葡萄糖转运蛋白1(GLUT1)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose Transporter 1 (GLUT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose Transporter 1 (GLUT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose Transporter 1 (GLUT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose Transporter 1 (GLUT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

生长激素受体(GHR)检测试剂盒适用生物     Homo sapiens (Human，人)    生长激素受体(GHR)检测试剂盒    检测范围     1.56-100ng/mL     灵敏度     0.57ng/mL    样本类型     Tissue homogenates, cell lysates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法    生长激素受体(GHR)检测试剂盒规格     96T    ELISA Kit for Growth Hormone Receptor (GHR)生长激素受体(GHR)检测试剂盒    FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEB036Hu    Sample type     Tissue homogenates, cell lysates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     1.56-100ng/mL The standard curve concentrations used for the ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL, 1.56ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.57ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Growth Hormone Receptor (GHR). No significant cross-reactivity or interference between Growth Hormone Receptor (GHR) and analogues was observed.生长激素受体(GHR)检测试剂盒  PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone Receptor (GHR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone Receptor (GHR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone Receptor (GHR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone Receptor (GHR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone Receptor (GHR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Hormone Receptor (GHR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

粘蛋白4(MUC4)检测试剂盒适用生物     Homo sapiens (Human，人)    粘蛋白4(MUC4)检测试剂盒    检测范围     0.313-20ng/mL     灵敏度     0.134ng/mL    样本类型     Serum, plasma, tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     粘蛋白4(MUC4)检测试剂盒规格     96T    ELISA Kit for Mucin 4 (MUC4)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEB034Hu    粘蛋白4(MUC4)检测试剂盒Sample type     Serum, plasma, tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.134ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Mucin 4 (MUC4). No significant cross-reactivity or interference between Mucin 4 (MUC4) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Mucin 4 (MUC4) and the recovery rates were calculated by comparing the measured value to the expected amount of Mucin 4 (MUC4) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     93-101     96    EDTA plasma(n=5)     91-98     95    heparin plasma(n=5)     88-96     92    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 4 (MUC4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 4 (MUC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mucin 4 (MUC4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     98-105%     90-98%     95-104%     78-99%    EDTA plasma(n=5)     96-103%     78-91%     83-97%     98-105%    heparin plasma(n=5)     86-99%     91-99%     78-94%     84-97%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 4 (MUC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 4 (MUC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 4 (MUC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 4 (MUC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

膜联蛋白A5(ANXA5)检测试剂盒适用生物 Rattus norvegicus (Rat，大鼠) 检测范围 0.156-10ng/mL 灵敏度 0.063ng/mL 样本类型 Serum, plasma and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 膜联蛋白A5(ANXA5)检测试剂盒ELISA Kit for Annexin A5 (ANXA5)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Rattus norvegicus (Rat)    Product No.     SEA259Ra    Sample type     Serum, plasma and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.063ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Annexin A5 (ANXA5). No significant cross-reactivity or interference between Annexin A5 (ANXA5) and analogues was observed.膜联蛋白A5(ANXA5)检测试剂盒RecoveryMatrices listed below were spiked with certain level of recombinant Annexin A5 (ANXA5) and the recovery rates were calculated by comparing the measured value to the expected amount of Annexin A5 (ANXA5) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     90-102     95    EDTA plasma(n=5)     90-104     101    heparin plasma(n=5)     96-104     101    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A5 (ANXA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A5 (ANXA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV膜联蛋白A5(ANXA5)检测试剂盒LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Annexin A5 (ANXA5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     98-105%     80-99%     91-99%     94-104%    EDTA plasma(n=5)     78-95%     98-105%     96-105%     89-97%    heparin plasma(n=5)     85-93%     78-94%     85-104%     95-105%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 膜联蛋白A5(ANXA5)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A5 (ANXA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A5 (ANXA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A5 (ANXA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A5 (ANXA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

蛋白激酶Cε(PKCε)检测试剂盒适用生物 Homo sapiens (Human，人) 检测范围 1.56-100ng/mL 灵敏度 0.55ng/mL 样本类型 Tissue homogenates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 蛋白激酶Cε(PKCε)检测试剂盒ELISA Kit for Protein Kinase C Epsilon (PKCe)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEA439Hu    Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     1.56-100ng/mL The standard curve concentrations used for the ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL, 1.56ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.55ng/mL.    蛋白激酶Cε(PKCε)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase C Epsilon (PKCe). No significant cross-reactivity or interference between Protein Kinase C Epsilon (PKCe) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase C Epsilon (PKCe) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase C Epsilon (PKCe) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    蛋白激酶Cε(PKCε)检测试剂盒Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 蛋白激酶Cε(PKCε)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Epsilon (PKCe). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Epsilon (PKCe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Epsilon (PKCe), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase C Epsilon (PKCe) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

有时候癌症研究者们会考虑将来自“死亡之帽”毒蘑菇衍生的毒性α-鹅膏蕈碱作为一种潜在的癌症疗法药物，然而由于α-鹅膏蕈碱会存在引发肝脏毒性的可能，因此其作为有效的疗法往往被认为存在很多限制。近日一篇刊登在国际杂志Nature上的研究论文中，来自得克萨斯大学MD癌症研究中心的研究人员着眼于开发一种以α-鹅膏蕈碱为基础的抗体共轭药物（ADCs），他们发现ADCs可以以POLR2A基因为靶点来来有效治疗患结直肠癌的小鼠模型，这种药物会有效促进肿瘤消退以及大幅降低自身的毒性，同时ADCs还会改善对癌细胞的靶向作用，尽可能地减少对健康细胞的影响。Xiongbin Lu教授表示，当传统的肿瘤抑制基因TP53被剔除后就会引发癌症发展，而其附近的基因POLR2A同时也会被剔除；正常细胞中拥有两个拷贝的POLR2A和TP53基因，而本文研究中研究者对含有单一拷贝两个基因的癌细胞进行研究，单一拷贝的两个基因在53%的结直肠癌、62%的乳腺癌以及75%的卵巢癌中存在。POLR2A是一种包括癌细胞在内的细胞生存的必须基因，由于其仅存在单一拷贝，因此癌细胞对于该基因的抑制会异常敏感。随着POLR2A和TP53基因同时被剔除就意味着靶向作用这两个基因遗传过程的疗法变得不再那么有效，从而使得癌细胞继续旺盛成长；而揭示单一拷贝的POLR2A基因或许就可以促进癌症发展，帮助科学家们寻找新型靶点来进行癌症治疗；文章中研究者检测了ADCs的作用，发现其可以有效抑制POLR2A基因的表达，进而抑制癌症的发展。在癌症疗法中研究者们花费了很大努力来试图恢复TP53的活性，然而由于TP53信号的复杂性，目前并没有基于TP53的疗法成功转化进入临床的癌症疗法阶段。POLR2A基因可以编码一种酶类，该酶类会被α-鹅膏蕈碱所以只，研究者发现，低剂量α-鹅膏蕈碱引发的基因POLR2A的抑制会阻断癌细胞的生长，并且降低毒性。最后研究者Lu说道，我们预测，抑制基因POLR2A的表达或许可以作为一种新型的治疗手段来治疗携带常见基因突变的人类癌症。

近期，发表在国际杂志Cell上的一篇研究论文中，来自马萨诸塞大学医学院的研究人员通过研究开发了一种新型强大的工具，其可以揭示和人类疾病相关的遗传突变的特性，相关研究或可帮助科学家轻松有效地描述隐藏在复杂多基因疾病背后的基因组突变。研究者Marian Walhout博士表示，截至目前为止我们仅可以在同一时间调查一种突变引发的疾病，而如今利用我们开发的平台就可以帮助我们在进行单一实验的情况下对成百上千种突变进行展现和分析，同时我们还可以绘制出一种新型的反应图谱，来可以组成强大的控制基因表达的网络来帮助确定基因突变如何引发多种人类疾病的发生。全基因组关联分析(Genome Wide Association Study，GWAS)为基因组测序带来了革命性的进步，其同时也帮助科学家们定位到了和多种疾病发生相关的多种遗传突变，然而这些突变中有90%都不能编码蛋白的产生；DNA结合位点或转录因子的突变往往会通过破坏基因的调节来引发疾病，而这往往会导致靶向蛋白产生过多或不足，多种疾病，比如癌症、神经变性疾病、血液障碍及代谢性疾病就和异常的基因调节直接相关。由GWAS鉴别出的大多数和疾病相关的突变实际上并不会改变蛋白质本身，而位于调节性区域的突变才会真正改变蛋白质的水平，于是研究者所面临的挑战就是鉴别这些突变如何以及为何引发疾病的发生。早在2011年研究者就开发出了一种增强的，以基因为中心的酵母杂交实验，于是研究者在本文中就进行了涉及1000种转录因子以及100多种基因突变的数千种实验，最后在多种相关疾病中发现了DNA位点间相互作用的缺失及获得情况，同似乎还鉴别出了和基因表达改变相关的转录因子。最后研究者Walhout说道，本文中新型工具平台的开发帮助我们深入探寻了遗传突变及转录因子之间相互作用的差异，其在干扰基因调节网络上扮演着重要作用，同时也帮助我们深入理解了这些网络同疾病发生之间的密切关系，为后期开发新型靶向疗法来治疗基因突变相关的疾病提供了新的研究思路和线索。

60kD热休克蛋白(HSPD1)检测试剂盒适用生物 Homo sapiens (Human，人) 检测范围 0.156-10ng/mL 灵敏度 0.062ng/mL 样本类型 Serum, plasma and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 60kD热休克蛋白(HSPD1)检测试剂盒ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEA822Hu    Sample type     Serum, plasma and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.062ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Heat Shock 60kD Protein 1, Chaperonin (HSPD1). No significant cross-reactivity or interference between Heat Shock 60kD Protein 1, Chaperonin (HSPD1) and analogues was observed.60kD热休克蛋白(HSPD1)检测试剂盒RecoveryMatrices listed below were spiked with certain level of recombinant Heat Shock 60kD Protein 1, Chaperonin (HSPD1) and the recovery rates were calculated by comparing the measured value to the expected amount of Heat Shock 60kD Protein 1, Chaperonin (HSPD1) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     98-105     102    EDTA plasma(n=5)     91-99     95    heparin plasma(n=5)     79-89     83    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 60kD Protein 1, Chaperonin (HSPD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 60kD Protein 1, Chaperonin (HSPD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV60kD热休克蛋白(HSPD1)检测试剂盒LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heat Shock 60kD Protein 1, Chaperonin (HSPD1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     85-95%     87-101%     78-103%     95-104%    EDTA plasma(n=5)     79-102%     95-105%     94-105%     99-105%    heparin plasma(n=5)     96-104%     97-105%     93-102%     90-97%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 60kD Protein 1, Chaperonin (HSPD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 60kD Protein 1, Chaperonin (HSPD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 60kD Protein 1, Chaperonin (HSPD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 60kD Protein 1, Chaperonin (HSPD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

2015上海沪震五一放假通知                               尊敬的客户，公司各职员：       您们好！感谢大家长期关注上海沪震生物动态，根据国家法定假期的规定，并结合公司实际情况，现对五一节放假做如下安排：一、放假时间5月1日至3日(星期五至星期天)放假，共3天。其中，5月1日(星期五)为五一假期，5月2日(星期六)，5月3日(星期天)为法定节假日照常公休。二、请公司各职员做好自己的节前工作安排。三、放假期间本公司不安排人员值班，如有订购产品请发送留言至我司网站平台，我们会在节后上班第一时间及时为您办理订货发货。各网站均有在线留言咨询栏。对此给您带来的不便我们深感抱歉。感谢你的支持与理解。                                                             上海沪震 特此通知! 

4月29日， 春晚福娃邓鸣贺的爷爷上午向记者证实，邓鸣贺因白血病复发去世，全家昨晚因孩子去世已经回到河北邯郸。爷爷因心情极度悲伤婉拒了记者更多采访。据微博@韩鹏飞Forrest 消息，《梨园春》明星小擂主邓鸣贺，因白血病复发，于28日晚抢救无效去世。2012年，他曾以小福娃的形象登上春晚的舞台。两度上春晚2012年龙年春晚开门娃娃。登上2013年中央电视台春节联欢晚会，参与开场童谣和零点敲钟两个重要环节 。 2013蛇年央视春晚上，童星邓鸣贺和妹妹邓鸣璐表演了创意节目《剪花花》，给观众留下深刻印象。2013年元宵晚会的创意阶段，曾为邓鸣贺设计了全新的节目。2013年2月22日傍晚确认罹患急性非淋巴细胞白血病。邓鸣贺的爷爷在电话中告诉记者，小鸣贺2012年在学校体检时还一切正常，但在2013年春晚表演《剪花花》时，就已开始发烧，当时他坚持表演完节目。结果回到家后，邓鸣贺一直没有退烧，父母在大年初四带着他从河北到北京看病。之后邓鸣贺已经住院并已退烧，他并不知道自己得了什么病，每天还是很开心。获得善款2013年3月5日， 被北京儿童医院确诊患有急性髓细胞白血病的童星邓鸣贺收到两家企业的200万元善款。北京儿童医院表示，邓鸣贺已完成第一阶段化疗，病情稳定。捐款仪式现场，邓鸣贺的爷爷邓庆华数度落泪，站起来鞠躬答谢。他表示，所有善款都将存入北京儿童医院基金会，待邓鸣贺病愈后，未用完的善款将悉数用于其他孩子的治疗。

沉默调节蛋白2(SIRT2)检测试剂盒适用生物 Homo sapiens (Human，人)  检测范围 1.56-100ng/mL 灵敏度 0.55ng/mL 样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 沉默调节蛋白2(SIRT2)检测试剂盒  ELISA Kit for Sirtuin 2 (SIRT2)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species Homo sapiens (Human) Product No. SEA430Hu Sample type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Format 96-well strip plate Assay length 4.5 hours Detection range 1.56-100ng/mL The standard curve concentrations used for the ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL, 1.56ng/mL  Sensitivity The minimum detectable dose of this kit is typically less than 0.55ng/mL.沉默调节蛋白2(SIRT2)检测试剂盒  SpecificityThis assay has high sensitivity and excellent specificity for detection of Sirtuin 2 (SIRT2). No significant cross-reactivity or interference between Sirtuin 2 (SIRT2) and analogues was observed. RecoveryMatrices listed below were spiked with certain level of recombinant Sirtuin 2 (SIRT2) and the recovery rates were calculated by comparing the measured value to the expected amount of Sirtuin 2 (SIRT2) in samples. Matrix Recovery range (%) Average(%) serum(n=5) 80-101 84 EDTA plasma(n=5) 88-102 98 heparin plasma(n=5) 89-97 92 PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sirtuin 2 (SIRT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sirtuin 2 (SIRT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV沉默调节蛋白2(SIRT2)检测试剂盒   LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sirtuin 2 (SIRT2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 90-99% 80-101% 80-93% 92-104% EDTA plasma(n=5) 83-95% 84-98% 88-102% 98-105% heparin plasma(n=5) 81-103% 82-90% 90-98% 88-95% StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Reagents and materials providedReagents Quantity Reagents Quantity Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4 Standard 2 Standard Diluent 1×20mL Detection Reagent A 1×120μL Assay Diluent A 1×12mL Detection Reagent B 1×120μL Assay Diluent B 1×12mL TMB Substrate 1×9mL Stop Solution 1×6mL Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1 Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sirtuin 2 (SIRT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sirtuin 2 (SIRT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sirtuin 2 (SIRT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sirtuin 2 (SIRT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

回肠脂肪酸结合蛋白(FABP6)检测试剂盒适用生物 Homo sapiens (Human，人) 检测范围 0.156-10ng/mL 灵敏度 0.055ng/mL 样本类型 Serum, plasma and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 回肠脂肪酸结合蛋白(FABP6)检测试剂盒  ELISA Kit for Fatty Acid Binding Protein 6, Ileal (FABP6)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species Homo sapiens (Human) Product No. SEA344Hu Sample type Serum, plasma and other biological fluids. Format 96-well strip plate Assay length 4.5 hours Detection range 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL  Sensitivity The minimum detectable dose of this kit is typically less than 0.055ng/mL.回肠脂肪酸结合蛋白(FABP6)检测试剂盒 SpecificityThis assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 6, Ileal (FABP6). No significant cross-reactivity or interference between Fatty Acid Binding Protein 6, Ileal (FABP6) and analogues was observed. RecoveryMatrices listed below were spiked with certain level of recombinant Fatty Acid Binding Protein 6, Ileal (FABP6) and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Binding Protein 6, Ileal (FABP6) in samples. Matrix Recovery range (%) Average(%) serum(n=5) 81-91 87 EDTA plasma(n=5) 88-102 92 heparin plasma(n=5) 97-105 101 回肠脂肪酸结合蛋白(FABP6)检测试剂盒 PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 6, Ileal (FABP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 6, Ileal (FABP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Binding Protein 6, Ileal (FABP6) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 95-103% 85-102% 80-97% 99-105% EDTA plasma(n=5) 90-105% 89-97% 78-90% 78-90% heparin plasma(n=5) 89-96% 79-93% 80-93% 91-103% StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Reagents and materials providedReagents Quantity Reagents Quantity Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4 Standard 2 Standard Diluent 1×20mL Detection Reagent A 1×120μL Assay Diluent A 1×12mL Detection Reagent B 1×120μL Assay Diluent B 1×12mL TMB Substrate 1×9mL Stop Solution 1×6mL Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1 Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 6, Ileal (FABP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 6, Ileal (FABP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 6, Ileal (FABP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Binding Protein 6, Ileal (FABP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

糖抗原125(CA125)检测试剂盒适用生物 Mus musculus (Mouse，小鼠) 检测范围 15.63-1000pg/mL 灵敏度 6.6pg/mL 样本类型 Serum, plasma, tissue homogenates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T糖抗原125(CA125)检测试剂盒(酶联免疫吸附试验法)适用生物：小鼠使用说明书仅供体外研究使用，不用于临床诊断!糖抗原125(CA125)检测试剂盒[预期应用]本试剂盒运用双抗体夹心ELISA 法定量测定小鼠血清、血浆、组织匀浆或其它相关生物液体中CA125 含量。[试剂盒内容]试剂名称数量试剂名称数量96孔板(预包被) 1 96孔板覆膜4标准品2 标准品稀释液1×20mL检测溶液A 1×120μL 检测稀释液A 1×12mL检测溶液B 1×120μL 检测稀释液B 1×12mLTMB底物1×9mL 终止液1×6mL浓洗涤液(30×) 1×20mL 使用说明书1[需自备的设备及试剂]1、450±10nm 滤光片的酶标仪(建议仪器使用前提前预热)2、单道或多道微量加液器及吸头3、稀释样品的EP 管4、蒸馏水或去离子水5、吸水纸6、盛放洗液的容器[试剂盒的储存及有效期]1、未开封的试剂盒：所有试剂均按试剂瓶标签上所示保存。请注意，收到试剂盒后请尽快将标准品、检测溶液A、检测溶液B 以及96 孔板保存于-20oC。2、使用后的试剂盒：剩余试剂仍需按照试剂瓶标签所示的温度保存，开封后的酶标板要加干燥剂后密封保存于-20oC，避免潮湿。注意：试剂盒内酶标条可拆卸，按实验需求可分多次使用；使用后的剩余试剂盒建议在首次实验后1 个月内使用完毕。产品过期时间以盒子上的标签为准，保质期内所有组分都确保是稳定的。[标本的采集与保存]1、血清：将收集于血清分离管的全血标本在室温放置2 小时或4oC 过夜，然后1000×g 离心20 分钟，取上清即可，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。2、血浆：用EDTA 或肝素作为抗凝剂采集标本，并将标本在采集后的30 分钟内于2-8oC 1000×g 离心15 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。3、组织匀浆：1） 取适量组织块，于预冷PBS（0.01mol/L, pH 7.0-7.2）中清洗去除血液，称重后备用（组织块较大需先剪碎后再匀浆）；2） 可同时选用多种匀浆方法达到较好的破碎效果：首先将组织块移入玻璃匀浆器，加入5-10mL 预冷PBS 进行充分研磨，该过程需在冰上进行（有条件实验室可选用机器匀浆）；得到的匀浆液可再利用超声破碎或反复冻融进一步处理（超声破碎过程中注意冰浴降温；反复冻融法可重复2 次）。3） 将制备好的匀浆液于5000×g 离心5 分钟，留取上清即可检测。4、其它生物标本：请1000×g 离心20 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。注意：1、以上标本均需密封保存，4oC 保存应小于1 周，-20oC 不应超过1 个月，-80oC 不应超过2 个月。2、标本溶血会影响最后检测结果，因此溶血标本不宜进行此项检测。3、标本使用前应缓慢均衡至室温，不应加热使之融解。[试剂准备]1、使用前将所有的试剂和标本缓慢均衡至室温(18-25oC)，试剂不能直接在37oC 溶解。2、标准品(冻干品)：每瓶标准品加入标准品稀释液1mL，盖好后室温静置大约10 分钟，同时反复颠倒/搓动以助溶解，其浓度为80ng/mL(贮液)。先将其稀释为20ng/mL(标准曲线最高浓度)后，再准备7 个稀释标准品的EP 管，每个EP 管中加入500μL 的标准品稀释液，如图所示依次倍比稀释成20ng/mL，10ng/mL，5ng/mL，2.5ng/mL，1.25ng/mL，0.625ng/mL，0.312ng/mL，标准品稀释液(0ng/mL)直接作为空白孔。为保证实验结果有效性，每次实验请使用新的标准品溶液。3、检测溶液A 及检测溶液B：Detection A 及Detection B 在使用前请手甩几下或少时离心处理，以使管壁或瓶盖的液体沉积到管底。临用前分别以检测稀释液A 或B1:100 稀释(如：10μL 检测溶液A/990μL 检测稀释液A)，充分混匀，稀释前根据预先计算好的每次实验所需的总量配制(100μL/孔)，实际配制时应多配制0.1-0.2mL。4、浓洗涤液：用580mL 蒸馏水或去离子水将20mL 浓洗涤液稀释至600mL，进行30 倍稀释。5、底物溶液：请用灭菌的移液器吸头吸取所需体积的TMB 至另一干净容器中使用，容器中剩余的底物应予丢弃，不要倒回TMB 瓶中。注意：1、标准品的稀释不能直接在板中进行。2、标准品请于临用前15 分钟内配制。该标准品只能使用一次。3、标准品、检测溶液A 工作液、检测溶液B 工作液请使用相应的稀释液配制，不能混淆。混匀时要轻轻充分混匀，避免起泡。为保证实验结果的准确请使用微量吸管，并校准微量加液器。请依据所需的量精确配制，尽量不要微量配制(如吸取检测溶液A 时，一次不要小于10μL)，以避免造成浓度误差。Tube 1 2 3 4 5 6 7 8 9ng/mL 80 20 10 5 2.5 1.25 0.625 0.312 04、请勿重复使用已经稀释过的标准品、检测溶液A 工作液和检测溶液B 工作液。5、浓洗涤液中如有结晶析出，请先温育至室温，轻轻混匀，至到结晶完全溶解再进行配制。6、试剂盒中部分试剂为储存液，客户需配置成工作液后使用，在配置过程中如因纯净水质量差或水质污染，以及实验中所用耗材洁净度差，可能造成实验结果不准确，甚至完全错误，请使用双蒸水。[标本处理]1、本公司只对试剂盒本身负责，不对因使用该试剂盒所造成的样本消耗负责，请使用者使用前充分考虑到样本的可能使用量，预留充足的样本。2、实验前应预测标本含量，如果标本浓度过高，应对标本进行稀释，使稀释后的标本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。标本使用0.01mol/L 的PBS 稀释(PH=7.0-7.2)。3、若所检样本不包含在说明书所列样本之中，建议进行预实验验证其有效性，并注意留存样本。4、使用化学裂解液制备的组织匀浆或细胞提取液可能会由于某些化学物质的引入导致ELISA 实验结果偏差。5、若样本为细胞培养上清，因该类样本干扰因素较多，如：细胞状态、细胞数量、采样时间等，所以可能存在检测不出的情况。6、某些天然蛋白或重组蛋白，包括原核及真核重组蛋白，可能因为与本产品所使用的检测抗体及捕获抗体不匹配，而不被检测出。7、建议使用新鲜样本，保存时间过长可能会存在蛋白降解或变性导致实验结果偏差。[操作步骤]1、加样：分别设标准孔、待测样品孔、空白孔。设标准孔7 孔，依次加入100μL 不同浓度的标准品(见试剂准备2)。空白孔加100μL(见试剂准备第二步最后一管)，余孔加待测样品100μL，酶标板加上覆膜，37oC 温育2 小时。2、弃去液体，甩干，不用洗涤。3、每孔加检测溶液A 工作液100μL(临用前配制)，酶标板加上覆膜，37oC 温育1 小时。4、弃去孔内液体，每孔用350μL 的洗涤液洗涤，浸泡1-2 分钟，吸去(不可触及板壁)或甩掉酶标板内的液体，在实验台上铺垫几层吸水纸，酶标板朝下用力拍几次(也可轻拍将孔内液体拍干)，重复洗板3 次。最后一次洗涤后，要把孔内的洗涤液完全甩干。自动洗板机亦可。5、每孔加检测溶液B 工作液(临用前配制)100μL，加上覆膜，37oC 温育30 分钟。6、弃去孔内液体，甩干，洗板5 次，方法同步骤4。7、每孔加底物溶液90μL，酶标板加上覆膜，37oC 避光显色(反应时间控制在15-25 分钟，不要超过30 分钟。当标准孔的前3-4 孔有明显的梯度蓝色，后3-4 孔梯度不明显时，即可终止)。8、每孔加终止溶液50μL，终止反应，此时蓝色立转黄色。终止液的加入顺序应尽量与底物溶液的加入顺序相同。如出现颜色不匀一，请轻轻晃动酶标板以使溶液混合均匀。9、在确保酶标板底无水滴及孔内无气泡后，立即用酶标仪在450nm 波长测量各孔的光密度(O.D.值)。注意：1、试剂准备：准备一次实验所需要的酶标条，其它的可从微孔板上拆下，密封，按照说明书要求保存，以备下次使用。2、加样：实验操作中请使用一次性的吸头，避免交叉污染。加样时注意不要有气泡，将样品加于酶标板底部，尽量不触及孔壁，轻轻晃动混匀。加样或加试剂时，第一个孔与最后一个孔加样之间的时间间隔如果太大，将会导致不同的“预温育”时间，从而明显地影响到测量值的准确性及重复性。因此，一次加样时间(包括标准品及所有样品)最好控制在10 分钟内。推荐设置复孔进行实验。3、温育：为防止样品蒸发，实验时请将加上盖或覆膜的酶标板置于湿盒内，以避免液体蒸发，洗板后应尽快进行下步操作，任何时侯都应避免酶标板处于干燥状态，同时应严格遵守给定的温育时间和温度。4、洗涤：充分的洗涤非常重要，在每次洗涤过程中，都要将洗涤液完全甩干。洗涤过程中反应孔中残留的洗涤液应在滤纸上拍干，勿将滤纸直接放入反应孔中吸水，同时要消除板底残留的液体和手指印，避免影响最后的酶标仪读数。如果有自动洗板机，应在熟练使用后再用到正式实验过程中。5、反应时间的控制：加入底物后请定时观察反应孔的颜色变化(比如，每隔10 分钟观察一次)，如颜色较深，请提前加入终止液终止反应，避免反应过强从而影响酶标仪光密度读数。6、底物：底物请避光保存，在储存和温育时避免强光直接照射。7、如果实验室内湿度低于60%，推荐使用加湿器提高湿度水平。[实验原理]将CA125 抗体包被于96 孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的CA125 与连接于固相载体上的抗体结合，然后加入生物素化的CA125 抗体，将未结合的生物素化抗体洗净后，加入HRP 标记的亲和素，再次彻底洗涤后加入TMB 底物显色。TMB 在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CA125 呈正相关。用酶标仪在450nm 波长下测定吸光度(O.D.值)，计算样品浓度。[计算]各标准品及样本O.D.值扣除空白孔O.D.值后作图(七点图)，如设置复孔，则应取其平均值计算。以标准品的浓度为纵坐标(或对数坐标)，O.D.值为横坐标(或对数坐标），绘出标准曲线(最佳方程式应依回归方程计算的R2值来定，以R2 值越趋近于1 为好)。推荐使用专业制作曲线软件进行分析，如curve expert 1.30，根据样品O.D.值，由标准曲线查出相应的浓度，乘以稀释倍数；或用标准物的浓度与O.D.值计算出标准曲线的回归方程式，将样品的O.D.值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。[典型数据]为了便于计算，尽管浓度为自变量而O.D.值为因变量，我们绘图时仍采用标准品的O.D.值作为横坐标(X 轴)，标准品的浓度为纵坐标(Y 轴)。同时为了试验结果的直观性，图中提供的是原始数据而非对数值。推荐使用对数值建立标准曲线图。由于实验操作条件的不同（如操作者、移液技术、洗板技术和温度条件等），标准曲线的O.D.值会有所差异。所提供的标准曲线仅供参考，实验者需要根据自已的实验建立标准曲线。小鼠糖抗原125(CA125)检测试剂盒标准曲线[检测范围]0.312ng/mL-20ng/mL[最低检测限]0.116ng/mL此值为20 个空白样品(即标准品稀释液)测定的平均值加二倍标准差所对应的浓度。[特异性]本试剂盒用于检测CA125，经检测与其它相似物质无明显交叉反应。由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。[回收率]分别于定值血清及血浆样本中加入一定量的CA125（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。样本回收率范围(%) 平均回收率(%)血清(n=5) 78-94 86EDTA 血浆(n=5) 90-99 95肝素血浆(n=5) 92-104 97[线性]在定值血清及血浆样本内加入适量的CA125，并倍比稀释成1:2，1:4，1:8，1:16 的待测样本，线性范围即为稀释后样本中CA125 含量的测定值与理论值的比率。样本1：2 1：4 1：8 1：16血清(n=5) 94-103% 88-99% 93-105% 83-94%EDTA 血浆(n=5) 83-96% 93-102% 85-97% 89-101%肝素血浆(n=5) 78-94% 90-99% 80-92% 93-107%[精密度]精密度用样品测定值的变异系数CV 表示。CV(%) = SD/mean×100批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD 值。批间差：选取3 个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8 次，分别计算不同浓度样本的平均值及SD 值。批内差： CV批间差： CV[稳定性]经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。[实验流程]1、实验前标准品、试剂及样本的准备；2、加样（标准品及样本）100μL，37oC孵育2小时；3、吸弃，加检测溶液A100μL，37oC孵育1小时；4、洗板3次；5、加检测溶液B100μL，37oC孵育30分钟；6、洗板5次；7、加TMB底物90μL，37oC孵育15－25分钟；8、加终止液50μL，立即450nm读数。 

血红素氧合酶1(HO1)检测试剂盒适用生物 Homo sapiens (Human，人) 检测范围 0.313-20ng/mL 灵敏度 0.127ng/mL 样本类型 Serum, plasma, tissue homogenates, cell lysates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T血红素氧合酶1(HO1)检测试剂盒(酶联免疫吸附试验法)适用生物：人使用说明书仅供体外研究使用，不用于临床诊断!血红素氧合酶1(HO1)检测试剂盒[预期应用]本试剂盒运用双抗体夹心ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液或其它相关生物液体中HO1 含量。[试剂盒内容]试剂名称数量试剂名称数量96孔板(预包被) 1 96孔板覆膜4标准品2 标准品稀释液1×20mL检测溶液A 1×120μL 检测稀释液A 1×12mL检测溶液B 1×120μL 检测稀释液B 1×12mLTMB底物1×9mL 终止液1×6mL浓洗涤液(30×) 1×20mL 使用说明书1[需自备的设备及试剂]1、450±10nm 滤光片的酶标仪(建议仪器使用前提前预热)2、单道或多道微量加液器及吸头3、稀释样品的EP 管4、蒸馏水或去离子水5、吸水纸6、盛放洗液的容器[试剂盒的储存及有效期]1、未开封的试剂盒：所有试剂均按试剂瓶标签上所示保存。请注意，收到试剂盒后请尽快将标准品、检测溶液A、检测溶液B 以及96 孔板保存于-20oC。2、使用后的试剂盒：剩余试剂仍需按照试剂瓶标签所示的温度保存，开封后的酶标板要加干燥剂后密封保存于-20oC，避免潮湿。注意：试剂盒内酶标条可拆卸，按实验需求可分多次使用；使用后的剩余试剂盒建议在首次实验后1 个月内使用完毕。产品过期时间以盒子上的标签为准，保质期内所有组分都确保是稳定的。[标本的采集与保存]1、血清：将收集于血清分离管的全血标本在室温放置2 小时或4oC 过夜，然后1000×g 离心20 分钟，取上清即可，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。2、血浆：用EDTA 或肝素作为抗凝剂采集标本，并将标本在采集后的30 分钟内于2-8oC 1000×g 离心15 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。3、组织匀浆：1） 取适量组织块，于预冷PBS（0.01mol/L, pH 7.0-7.2）中清洗去除血液，称重后备用（组织块较大需先剪碎后再匀浆）；2） 可同时选用多种匀浆方法达到较好的破碎效果：首先将组织块移入玻璃匀浆器，加入5-10mL 预冷PBS 进行充分研磨，该过程需在冰上进行（有条件实验室可选用机器匀浆）；得到的匀浆液可再利用超声破碎或反复冻融进一步处理（超声破碎过程中注意冰浴降温；反复冻融法可重复2 次）。3） 将制备好的匀浆液于5000×g 离心5 分钟，留取上清即可检测。4、细胞裂解液：1）贴壁细胞需要先用胰酶消化，离心收集细胞（悬浮细胞可直接离心收集）；2）将收集到的细胞用冷PBS 洗3 次；3）物理方法裂解细胞（可先超声破碎细胞，再反复冻融）：i 超声破碎：取适量PBS 重悬细胞，用一定功率的超声波处理细胞悬液，使细胞急剧震荡破裂。ii 反复冻融：将待破碎的细胞在-20oC 以下冰冻，室温融解，反复3 次，使细胞溶胀破碎。4）将标本于2-8oC 1500×g 离心10 分钟，收集上清备用。5、其它生物标本：请1000×g 离心20 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。注意：1、以上标本均需密封保存，4oC 保存应小于1 周，-20oC 不应超过1 个月，-80oC 不应超过2 个月。2、标本溶血会影响最后检测结果，因此溶血标本不宜进行此项检测。3、标本使用前应缓慢均衡至室温，不应加热使之融解。[试剂准备]1、使用前将所有的试剂和标本缓慢均衡至室温(18-25oC)，试剂不能直接在37oC 溶解。2、标准品(冻干品)：每瓶标准品加入标准品稀释液1mL，盖好后室温静置大约10 分钟，同时反复颠倒/搓动以助溶解，其浓度为80ng/mL(贮液)。先将其稀释为20ng/mL(标准曲线最高浓度)后，再准备7 个稀释标准品的EP 管，每个EP 管中加入500μL 的标准品稀释液，如图所示依次倍比稀释成20ng/mL，10ng/mL，5ng/mL，2.5ng/mL，1.25ng/mL，0.625ng/mL，0.312ng/mL，标准品稀释液(0ng/mL)直接作为空白孔。为保证实验结果有效性，每次实验请使用新的标准品溶液。Tube 1 2 3 4 5 6 7 8 9ng/mL 80 20 10 5 2.5 1.25 0.625 0.312 03、检测溶液A 及检测溶液B：Detection A 及Detection B 在使用前请手甩几下或少时离心处理，以使管壁或瓶盖的液体沉积到管底。临用前分别以检测稀释液A 或B1:100 稀释(如：10μL 检测溶液A/990μL 检测稀释液A)，充分混匀，稀释前根据预先计算好的每次实验所需的总量配制(100μL/孔)，实际配制时应多配制0.1-0.2mL。4、浓洗涤液：用580mL 蒸馏水或去离子水将20mL 浓洗涤液稀释至600mL，进行30 倍稀释。5、底物溶液：请用灭菌的移液器吸头吸取所需体积的TMB 至另一干净容器中使用，容器中剩余的底物应予丢弃，不要倒回TMB 瓶中。注意：1、标准品的稀释不能直接在板中进行。2、标准品请于临用前15 分钟内配制。该标准品只能使用一次。3、标准品、检测溶液A 工作液、检测溶液B 工作液请使用相应的稀释液配制，不能混淆。混匀时要轻轻充分混匀，避免起泡。为保证实验结果的准确请使用微量吸管，并校准微量加液器。请依据所需的量精确配制，尽量不要微量配制(如吸取检测溶液A 时，一次不要小于10μL)，以避免造成浓度误差。4、请勿重复使用已经稀释过的标准品、检测溶液A 工作液和检测溶液B 工作液。5、浓洗涤液中如有结晶析出，请先温育至室温，轻轻混匀，至到结晶完全溶解再进行配制。6、试剂盒中部分试剂为储存液，客户需配置成工作液后使用，在配置过程中如因纯净水质量差或水质污染，以及实验中所用耗材洁净度差，可能造成实验结果不准确，甚至完全错误，请使用双蒸水。[标本处理]1、本公司只对试剂盒本身负责，不对因使用该试剂盒所造成的样本消耗负责，请使用者使用前充分考虑到样本的可能使用量，预留充足的样本。2、实验前应预测标本含量，如果标本浓度过高，应对标本进行稀释，使稀释后的标本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。标本使用0.01mol/L 的PBS 稀释(PH=7.0-7.2)。3、若所检样本不包含在说明书所列样本之中，建议进行预实验验证其有效性，并注意留存样本。4、使用化学裂解液制备的组织匀浆或细胞提取液可能会由于某些化学物质的引入导致ELISA 实验结果偏差。5、若样本为细胞培养上清，因该类样本干扰因素较多，如：细胞状态、细胞数量、采样时间等，所以可能存在检测不出的情况。6、某些天然蛋白或重组蛋白，包括原核及真核重组蛋白，可能因为与本产品所使用的检测抗体及捕获抗体不匹配，而不被检测出。7、建议使用新鲜样本，保存时间过长可能会存在蛋白降解或变性导致实验结果偏差。[操作步骤]1、加样：分别设标准孔、待测样品孔、空白孔。设标准孔7 孔，依次加入100μL 不同浓度的标准品(见试剂准备2)。空白孔加100μL(见试剂准备第二步最后一管)，余孔加待测样品100μL，酶标板加上覆膜，37oC 温育2 小时。2、弃去液体，甩干，不用洗涤。3、每孔加检测溶液A 工作液100μL(临用前配制)，酶标板加上覆膜，37oC 温育1 小时。4、弃去孔内液体，每孔用350μL 的洗涤液洗涤，浸泡1-2 分钟，吸去(不可触及板壁)或甩掉酶标板内的液体，在实验台上铺垫几层吸水纸，酶标板朝下用力拍几次(也可轻拍将孔内液体拍干)，重复洗板3 次。最后一次洗涤后，要把孔内的洗涤液完全甩干。自动洗板机亦可。5、每孔加检测溶液B 工作液(临用前配制)100μL，加上覆膜，37oC 温育30 分钟。6、弃去孔内液体，甩干，洗板5 次，方法同步骤4。7、每孔加底物溶液90μL，酶标板加上覆膜，37oC 避光显色(反应时间控制在15-25 分钟，不要超过30 分钟。当标准孔的前3-4 孔有明显的梯度蓝色，后3-4 孔梯度不明显时，即可终止)。8、每孔加终止溶液50μL，终止反应，此时蓝色立转黄色。终止液的加入顺序应尽量与底物溶液的加入顺序相同。如出现颜色不匀一，请轻轻晃动酶标板以使溶液混合均匀。9、在确保酶标板底无水滴及孔内无气泡后，立即用酶标仪在450nm 波长测量各孔的光密度(O.D.值)。注意：1、试剂准备：准备一次实验所需要的酶标条，其它的可从微孔板上拆下，密封，按照说明书要求保存，以备下次使用。2、加样：实验操作中请使用一次性的吸头，避免交叉污染。加样时注意不要有气泡，将样品加于酶标板底部，尽量不触及孔壁，轻轻晃动混匀。加样或加试剂时，第一个孔与最后一个孔加样之间的时间间隔如果太大，将会导致不同的“预温育”时间，从而明显地影响到测量值的准确性及重复性。因此，一次加样时间(包括标准品及所有样品)最好控制在10 分钟内。推荐设置复孔进行实验。3、温育：为防止样品蒸发，实验时请将加上盖或覆膜的酶标板置于湿盒内，以避免液体蒸发，洗板后应尽快进行下步操作，任何时侯都应避免酶标板处于干燥状态，同时应严格遵守给定的温育时间和温度。4、洗涤：充分的洗涤非常重要，在每次洗涤过程中，都要将洗涤液完全甩干。洗涤过程中反应孔中残留的洗涤液应在滤纸上拍干，勿将滤纸直接放入反应孔中吸水，同时要消除板底残留的液体和手指印，避免影响最后的酶标仪读数。如果有自动洗板机，应在熟练使用后再用到正式实验过程中。5、反应时间的控制：加入底物后请定时观察反应孔的颜色变化(比如，每隔10 分钟观察一次)，如颜色较深，请提前加入终止液终止反应，避免反应过强从而影响酶标仪光密度读数。6、底物：底物请避光保存，在储存和温育时避免强光直接照射。7、如果实验室内湿度低于60%，推荐使用加湿器提高湿度水平。[实验原理]将HO1 抗体包被于96 孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的HO1 与连接于固相载体上的抗体结合，然后加入生物素化的HO1 抗体，将未结合的生物素化抗体洗净后，加入HRP 标记的亲和素，再次彻底洗涤后加入TMB 底物显色。TMB 在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的HO1 呈正相关。用酶标仪在450nm 波长下测定吸光度(O.D.值)，计算样品浓度。[计算]各标准品及样本O.D.值扣除空白孔O.D.值后作图(七点图)，如设置复孔，则应取其平均值计算。以标准品的浓度为纵坐标(或对数坐标)，O.D.值为横坐标(或对数坐标），绘出标准曲线(最佳方程式应依回归方程计算的R2值来定，以R2 值越趋近于1 为好)。推荐使用专业制作曲线软件进行分析，如curve expert 1.30，根据样品O.D.值，由标准曲线查出相应的浓度，乘以稀释倍数；或用标准物的浓度与O.D.值计算出标准曲线的回归方程式，将样品的O.D.值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。[典型数据]为了便于计算，尽管浓度为自变量而O.D.值为因变量，我们绘图时仍采用标准品的O.D.值作为横坐标(X 轴)，标准品的浓度为纵坐标(Y 轴)。同时为了试验结果的直观性，图中提供的是原始数据而非对数值。推荐使用对数值建立标准曲线图。由于实验操作条件的不同（如操作者、移液技术、洗板技术和温度条件等），标准曲线的O.D.值会有所差异。所提供的标准曲线仅供参考，实验者需要根据自已的实验建立标准曲线。

组织因子通道抑制因子(TFPI)检测试剂盒适用生物 Homo sapiens (Human，人) 检测范围 0.156-10ng/mL 灵敏度 0.061ng/mL 样本类型 Plasma, urine, tissue homogenates, cell culture supernates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T ELISA Kit for Tissue Factor Pathway Inhibitor (TFPI)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEA394Hu    Sample type     Plasma, urine, tissue homogenates, cell culture supernates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.061ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Tissue Factor Pathway Inhibitor (TFPI). No significant cross-reactivity or interference between Tissue Factor Pathway Inhibitor (TFPI) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Tissue Factor Pathway Inhibitor (TFPI) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Factor Pathway Inhibitor (TFPI) in samples. Matrix     Recovery range (%)     Average(%)    sodium citrate plasma(n=5)     96-104     101    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor Pathway Inhibitor (TFPI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor Pathway Inhibitor (TFPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Factor Pathway Inhibitor (TFPI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    sodium citrate plasma(n=5)     91-104%     86-96%     84-103%     91-98%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Factor Pathway Inhibitor (TFPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Factor Pathway Inhibitor (TFPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Factor Pathway Inhibitor (TFPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tissue Factor Pathway Inhibitor (TFPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

生长激素(GH)检测试剂盒适用生物     Homo sapiens (Human，人)    检测范围     0.156-10ng/mL     灵敏度     0.064ng/mL    样本类型     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法   规格     96T    生长激素(GH)检测试剂盒      ELISA Kit for Growth Hormone (GH)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEA044Hu    Sample type     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.064ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH). No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.生长激素(GH)检测试剂盒RecoveryMatrices listed below were spiked with certain level of recombinant Growth Hormone (GH) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Hormone (GH) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     85-101     90    EDTA plasma(n=5)     94-103     98    heparin plasma(n=5)     90-104     94    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Hormone (GH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     79-102%     79-90%     89-102%     92-105%    EDTA plasma(n=5)     89-96%     78-93%     87-96%     80-90%    heparin plasma(n=5)     87-96%     84-94%     87-101%     94-101%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone (GH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone (GH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone (GH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Hormone (GH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

一项临床研究指出，通过计算机辅助调整（Clinical Decision Support CDS）肥胖儿童的日常生活状态有助于提高他们的体重指数（BMI）。 "我们的研究与之前的一系列研究结果一致：计算机化的CDS能够提升肥胖治疗的效果，"该研究的作者之一，麻省总医院儿科主任Elsie M. Taveras博士在文章中写道。Taveras博士同时也是哈佛医学院儿科与群体医学系的副教授。该研究结果发表在4月20日的JAMA儿科杂志上。 该项研究包括了年龄介于6到12岁的549名儿童，他们分别参加了麻省的14项治疗项目。所有儿童的基础BMI分布于95%或以上水平。这些项目被随机分为三组，其中的5个项目中儿童被要求遵循计算机化的CDS治疗方案。5个项目中儿童不仅要求遵循该方案，而且要求接受个体化的指导，另外4组项目中儿童接受的是常规方案。 一年以后，研究者发现仅接受计算机化的CDS方案的儿童BMI增长率低于常规治疗的儿童。另外，作者发现接受计算机化的CDS外加个体化的优化指导的儿童BMI相对于常规治疗方案有明显的提升。 在CDS组中，当儿童的BMI位于群体的95%或以上时，电子健康记录能够利用计算机进行提醒，同时还带有生长表格以及基于实验的肥胖筛查与治疗的指导方法。该计算机化的仪器还能够设计最佳的营养摄取方案与运动方案（基于实验获得）。医生也能够在该治疗过程中采取积极的配合性治疗方法。 另外，研究者们还针对个人与家庭的日常活动开发出了指导性的材料，在患者就医的时候可以使用。这些材料针对记录时间，糖类饮料的摄取，运动情况，睡眠等信息。

丹麦流传着一种说法，一户人家屋檐上的鹳巢数量与这家人所生孩子的数量存在着相关性。婴儿是鹳鸟送来的古老传说是真的吗？当然不是。相关性跟因果关系不是一回事。鹳不会送来孩子，但大房子有更大的空间为孩子和鹳所用。这是一则人们喜闻乐见的统计趣闻，但如果你知道1965年在美国参议院一场听证会上它是如何被用到的，你就不会觉得那么有趣了。那位做听证发言的专家证人辩称，尽管吸烟或许跟肺癌相关，但两者之间不存在已证明的、令人信服的因果关系。当被问及为何把鹳和孩子的关系与香烟和肺癌的关系进行类比，他回答说，两者“在我看来是一样的”。这位证人的名字叫达莱尔·哈夫(Darrell Huff)，是一名自由记者，因其1954年出版的那本精彩、大为畅销的《统计数字会撒谎》(How to Lie with Statistics)而深受数代极客的爱戴。如果该书续集付印的话，他今天的名声或许会完全不同。《吸烟统计数字会撒谎》(How to Lie with Smoking Statistics)使用了各种鹳式论点来对吸烟与癌症的相关性提出质疑。该书得到了美国的烟草研究所(Tobacco Institute)资助，但不知出于什么原因一直没有出版。（2012年安德鲁·格尔曼(Andrew Gelman)在《Chance》杂志上发表的文章，以及2014年亚历克斯·莱因哈特(Alex Reinhart)在《Significance》杂志上发表的文章，使哈夫担任烟草业顾问的经历引起统计学家们的注意。）毋庸置疑，吸烟会导致肺癌和其他多种致命疾病。但广泛意义上的相关性与因果之间的尚存疑问的关系，仍是当前一个极易引起争议和混淆的领域。哈佛大学(Harvard)法学院学生泰勒·维根(Tyler Vige)编撰并发布在其网站(tylervigen.com)上的“伪相关”应算是一种警告。你知道缅因州人造奶油的消费量与离婚率之间存在很强的相关性吗？所以，我们不能仅仅依赖相关性。但是，坚持为因果关系提供绝对证据就过于苛刻了（甚至是一种不可能达到的标准）。在这两个极端之间，如何在相信相关性与寻找因果证据之间达到合理的平衡呢？科学家、经济学家和统计学家倾向于要求为他们看到的现象提出因果解释。知道大学毕业生能赚更多钱还不够，我们想知道，大学教育是否提高了他们的收入，或者他们本来就是聪明人、不管接受大学教育与否都能赚更多钱。仅仅寻找相关性并非严格科学的做法。但随着“大数据”的到来，这场争论开始发生变化。海量数据集可以产生一些有趣的相关性，在某些用途上它们就足够好用了（谁关心为何周二降价效果最好呢？如果确是这样，那就选这一天降价。）英国央行(BoE)首席经济学家安德鲁·霍尔丹(Andy Haldane)不久前表示，经济学家们或许想更认真地看待纯粹相关性(mere correlation)。他不是第一个这么说的大数据热衷者。我们回头来讲抽烟与癌症之间的关系。20世纪40年代末，英国流行病学家理查德？多尔(Richard Doll)最早开始怀疑二者之间的联系。当时他的分析基于纯粹相关性，他不清楚因果机制，因为当时还没确定烟草中的大多数致癌物。多尔本人怀疑肺癌的致病原因是柏油公路的烟气，或者可能就是汽车本身。多尔与奥斯汀·布拉德福德·希尔(Austin Bradford Hill)在1950年发表了他们关于吸烟与癌症关系的早期研究结果，由于俩人的研究基于纯粹相关性，在当时果不其然遭到了批评。伟大的统计学家罗纳德·费雪(Ronald Fisher)在20世纪50年代多次加入论战，指出很可能是癌症引起吸烟，毕竟癌前期病变会对肺部造成刺激，人们可能会通过吸烟来缓解这一刺激。费雪还认为有些遗传特征可能既会引发肺癌，还会引起吸烟倾向。（另一位统计学家约瑟夫·伯克森(Joseph Berkson)提出，假如一个人强悍到足以抵制广告的诱惑和同龄人的压力，那么他也强悍到足以抵抗肺癌。）希尔和多尔的例子告诉我们，不要轻易否定相关性，但他们也以行动证明，不应放弃寻找因果解释。俩人继续勤恳研究，很快就发现了更多表明因果关系的证据。希尔和多尔在寻找因果关系时采取了一种务实的方法。比如，是否存在一种剂量效应？是的，烟瘾大的人更可能患肺癌。烟龄长短有关系吗？有关系，吸烟者开始吸烟很久后，癌细胞开始形成。这与费舍尔设想的人们在肺癌早期阶段用烟草进行自我医疗的假设相矛盾。多个证据来源凑到一起能否得到一个逻辑连贯的描述？答案是：能够得到。当医生们听闻希尔和多尔的发现时，许多医生开始戒烟，现实情况也表明戒烟者患肺癌的风险要更低。我们应该尊重相关性，但相关性只是通向更深层真理的一个线索，而不是研究的终点。目前尚不清楚为什么面对越来越多的吸烟致癌的证据，赫夫和费雪却执着地认为这仅是相关性。他们二人都是烟草行业的顾问，因而有些人会认为他们的怀疑动机来源于顾问费。但也很可能正是他们的怀疑带来了顾问费。到底哪个为因，哪个为果，后人可能永远不得而知。