大鼠柠檬酸合酶(CS)检测试剂盒 ,缩写英文名称: Rat Citrate synthase ,商用名称: 大鼠柠檬酸合酶(CS)检测试剂盒 ,商用别名: 大鼠柠檬酸合酶(CS)检测试剂盒 ,规格: 48T/96T
大鼠柠檬酸合酶(CS)检测试剂盒 反应时间:1-5h
实验方法:竞争法、一步法、间接法等
包装重量:860g(96T)、560g(48T)
本法不仅可以用来测定抗体,而且也可用于测定体液中的循环抗原,所以也是一种早期诊断的良好方法。因此法在生物医学各领域的应用范围日益扩大,
大鼠柠檬酸合酶(CS)检测试剂盒 可概括四个方面:
1、免疫酶染色各种细胞内成份的定位。
2、研究抗酶抗体的合成。
3、显现微量的免疫沉淀反应。
4、定量检测体液中抗原或抗体成份。
大鼠柠檬酸合酶(CS)检测试剂盒 洗板方法:
1. 自动洗板:如果有自动洗板机,应在熟练使用后再用到正式实验过程中。
2. 手工洗板方法:吸去(不可触及板壁)或甩掉酶标板内的液体;在实验台上铺垫几层吸水纸,酶标板朝下用力拍几次;将推荐的洗涤缓冲液至少0.3ml注入孔内,浸泡1-2分钟,根据需要,重复此过程数次。
计算:以标准物的浓度为纵坐标(对数坐标),OD值为横坐标(对数坐标),在对数坐标纸上绘出标准曲线。推荐使用专业制作曲线软件进行分析,如curve expert 1.3,根据样品的OD值由标准曲线查出相应的浓度,再乘以稀释倍数;或用标准物的浓度与OD值计算出标准曲线的回归方程式,将样品的OD值代入方程式,计算出样品浓度,再乘以稀释倍数,即为样品的实际浓度。
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