ZZK-K-ACET,乙酸检测试剂盒,Acetic Acid (ACS Manual Format) assay Kit
产品识别号:ZZK-K-ACET
产品品名:乙酸检测试剂盒
英文品名:Acetic Acid (ACS Manual Format) assay Kit
规格型号:53 Assays per Kit
A simple method for the rapid and reliable measurement of aceticacid/acetate in foods, beverages and other materials.
INTRODUCTION:
Acetic acid (acetate) occurs in a wide range of foods and beverages,
and in many other materials such as paper, pharmaceuticals and
industrial products. In the wine industry it is one of the most
important quality parameters and is measured throughout the entire
vinification process. This advanced kit (K-ACET) benefits from
greatly increased shelf life as the acetyl-coenzyme A synthetase
(ACS) is supplied as a highly stable ready-to-use ammonium sulphate
suspension, rather than as a lyophilised powder. Polyvinylpyrollidone
(PVP) has also been incorporated into the assay to prevent
interference from particular tannins found especially in red wines.
This kit is recommended for manual analysis, while two additional kits
have been optimised for auto-analysis applications; K-ACETAF has
extended calibration linearity up to 30 μg/mL (in the final assay), and
also benefits from a stabilisation system that significantly reduces the
rate of deterioration (increase in absorbance) of prepared reagent,
a problem exhibited by some other products; K-ACETAK is a new
assay format based on acetate kinase. As an indicator reaction is
not involved in this case, a change in absorbance stoichiometric with
the amount of acetic acid is achieved, resulting in excellent linear
calibration (R2 > 0.999).
KITS:
Kits suitable for performing 53 determinations are available from
Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (30 mL, pH 8.4) plus L-malic acid and sodium
azide (0.02 % w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: (x2) NAD+ plus ATP, PVP and CoA.
Freeze dried powder.
Stable for > 5 years at -20°C.
Bottle 3: L-Malate dehydrogenase plus citrate synthase
suspension, 1.1 mL.
Stable for > 2 years at 4°C.
Bottle 4: Acetyl-coenzyme A synthetase suspension (1.1 mL).
Stable for > 2 years at 4°C.
Bottle 5: Acetic acid standard solution (5 mL, 0.10 mg/mL).
Stable for > 2 years at 4°C.
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