Porcine CSFV Ag ELISA Kit
FOR RESEARCH USE ONLY
96 determinations
Purpose
This kit allows for the determination of CSFV Ag concentrations in Porcine
serum, and other biological fluids.
Principle of the assay
The kit assay CSFV Ag level in the sample,use Purified CSFV antibody to
coat microtiter plate wells, make solid-phase antibody, then add CSFV Ag to
wells, Combined With CSFV Ag, after washing and removing non-combinative
antibody and other components ,then Combined CSFV antibody which with
HRP labeled become antibody – antigen - enzyme- antibody complex, after
washing Completely, Add TMB substrate solution,, TMB substrate becomes
blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of
a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm. Compared with the
CUTOFF value, according to this to judge CSFV Ag exist in the sample or not.
Materials provided with the kit
1 wash solution 20ml×1bottle 7 Stop Solution 6ml×1 bottle
2 HRP-Conjugate
reagent 6ml×1 bottle 8 Positive control 0.5ml×1 bottle
3 Microelisa stripplate 12well×8strips 9 Negative
control
0.5ml×1bottl
e
4 Sample diluent 6ml×1 bottle 10 Instruction 1
5 Chromogen Solution
A 6ml×1 bottle 11 Closure plate
membrane 2
IBL International GmbH
Flughafenstra?e 52a 22335 Hamburg DE
IBL@IBL-International.com
6 Chromogen Solution
B 6ml×1 bottle 12 Sealed bags 1
Specimen requirements
1. extract as soon as possible after Specimen collection,and according to the
relevant literature, and should be experiment as soon as possible after the
extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid
repeated freeze-thaw cycles.
2. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP
active.
Assay procedure
1.Number: to sample correspond microtitration well and Number Sequence,
each plate should be set feminine comparison 2 wells, masculine comparison
2 wells, blank comparison 1 well(don’t add sample and HRP-Conjugate
reagent to blank comparison well, other each step the operation are same).
2.add sample:separately add Positive control and Negative control 50μl to the
Positive and Negative well . add Sample dilution 40μl to testing sample well,
then add testing sample 10μl. add sample to the bottom of ELISA plates
coated well , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30
min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold)
with distilled water until 600ml,and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing,
add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by
IBL International GmbH
Flughafenstra?e 52a 22335 Hamburg DE
IBL@IBL-International.com
pat.
6.add enzyme:Add HRP-Conjugate reagent 50μlto each well, except the blank
well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each
well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the
blue color change to yellow color).
11. assay:take blank well as zero , Read absorbance at 450nm after Adding
Stop Solution and within 15min.
Determine the result
Test validity: the average of Positive control well≥1.00; the average of
Negative control well ≤0.10.
Calculate Critical(CUT OFF) : Critical= the average of Negative control well
+ 0.15.
Negative control: sample OD< Calculate Critical(CUT OFF) is CSFV Ag
Negative control.
Positive control: ample OD≥ Calculate Critical(CUT OFF) is CSFV Ag
Positive control.
Important notes
IBL International GmbH
Flughafenstra?e 52a 22335 Hamburg DE
IBL@IBL-International.com
1.Please according to use instruction strictly, Do not mix reagents with those
from other lots.
2.The kit takes out from the refrigeration environment should be balanced
15-30 minutes in the room temperature then use, ELISA plates coated if
has not use up after opened, the plate should be stored in Sealed bag.
3.washing buffer will Crystallization separation, it can be heated the water
helps dissolve when dilute . Washing does not affect the result.
4.Closure plate membrane only limits the disposable use, in order to avoid
the overlapping pollution
5.The substrate please evade the light preservation.
6.The test result determination must take the microtiter plate reader as a
standard, when use dual-wavelength to assay, Reference wavelength is
630nm.
7.All samples, washing buffer and each kind of reject should according to
infective material process. Stopp Solution is 2M sulphuric acid. You must pay
attention to safe when use .
Storage and validity
1.Storage: 2-8℃.
2.validity: six months.
更多
实战分享|小鼠淋巴细胞的提取和分选之经验小结
厂商
2023.08.21
实战分享 | 原生细胞的合成之经验分享
厂商
2023.07.28
实验常用人肝癌细胞株(HepG2/Hep3B,HuH-7,MHCC97H,PLC/PRF/5)怎么选
厂商
2023.07.03
原代细胞的培养方法及注意事项
厂商
2023.06.26