1.标准品的稀释:本试剂盒提供原倍标准品一支,用户可按照下列图表在小试管中进行稀释。
120pg/ml
5号标准品
150μl的原倍标准品加入150μl标准品稀释液
60pg/ml
4号标准品
150μl的5号标准品加入150μl标准品稀释液
30pg/ml
3号标准品
150μl的4号标准品加入150μl标准品稀释液
15pg/ml
2号标准品
150μl的3号标准品加入150μl标准品稀释液
7.5pg/ml
1号标准品
150μl的2号标准品加入150μl标准品稀释液
2.加样:分别设空白孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)、标准孔、待测样品孔。在酶标包被板上标准品准确加样50μl,待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl(样品最终稀释度为5倍)。加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀。
3.温育:用封板膜封板后置37℃温育30分钟。
4.配液:将30倍浓缩洗涤液用蒸馏水30倍稀释后备用
5.洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。
6.加酶:每孔加入酶标试剂50μl,空白孔除外。
7.温育:操作同3。
8.洗涤:操作同5。
9.显色:每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.
10.终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。
11.测定:以空白空调零,450nm波长依序测量各孔的吸光度(OD值)。 测定应在加终止液后15分钟以内进行。
猪乙酰胆碱(ACh)ELISA试剂盒 Porcine acetylcholine,ACH ELISA试剂盒
猪主要组织相容性复合体Ⅲ类(MHCⅢ/SLAⅢ)ELISA试剂盒 swine major histocompatibility complexⅢ,MHCⅢ/SLAⅢ ELISA试剂盒
猪肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒 Porcine Pulmonary surfatcant-associated protein A,SP-A ELISA试剂盒
猪雌二醇受体(ER)ELISA试剂盒 Porcine Estradiol receptor,ER ELISA试剂盒
猪补体蛋白3(C3)ELISA试剂盒 Porcine Complement 3,C3 ELISA试剂盒
猪骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒 Porcine Osteocalcin/Bone gla protein,OT/BGP ELISA试剂盒
猪肾上腺素(EPI)ELISA试剂盒 Porcine Epinephrine/Adrenaline,EPI ELISA试剂盒
猪脂蛋白α(Lp-α)ELISA试剂盒 Porcine lipoprotein α,Lp-α ELISA试剂盒
猪促甲状腺素释放激素(TRH)ELISA试剂盒 Porcine thyrotropin-releasing hormone,TRH ELISA试剂盒
猪抗凝血酶受体(ATR)ELISA试剂盒 Porcine anti-thrombin receptor,ATR ELISA试剂盒
猪组胺(HIS)ELISA试剂盒 Porcine Histamine,HIS ELISA试剂盒
猪雌二醇(E2)ELISA试剂盒 Porcine Estradiol,E2 ELISA试剂盒
猪转化生长因子β1(TGF-β1)ELISA试剂盒 Porcine transforming growth factor β1,TGF-β1 ELISA试剂盒
猪肿瘤坏死因子α(TNF-α)ELISA试剂盒 Porcine tumor necrosis factor α,TNF-α ELISA试剂盒
猪脂联素(ADP)ELISA试剂盒 Porcine adiponectin,ADP ELISA试剂盒
猪载脂蛋白B100(apo-B100)ELISA试剂盒 Porcine apoprotein B100,apo-B100 ELISA试剂盒
猪载脂蛋白A1(apo-A1)ELISA试剂盒 Porcine apoprotein A1,apo-A1 ELISA试剂盒
猪胰岛素样生长因子结合蛋白3(IGFBP-3)ELISA试剂盒 Porcine insulin-like growth factors binding protein 3,IGFBP-3 ELISA试剂盒
猪胰岛素样生长因子2(IGF-2)ELISA试剂盒 Porcine insulin-like growth factors 2,IGF-2 ELISA试剂盒
猪胰岛素样生长因子1(IGF-1)ELISA试剂盒 Porcine insulin-like growth factors-1,IGF-1 ELISA试剂盒
猪胰岛素(INS)ELISA试剂盒 Porcine Insulin,INS ELISA试剂盒
猪血小板活化因子(PAF)ELISA试剂盒 Porcine platelet activating factor,PAF ELISA试剂盒
猪血管内皮细胞粘附分子1(VCAM-1/CD106)ELISA试剂盒 Porcine Vascuolar cell adhesion molecule 1,VCAM-1 ELISA试剂盒
猪血纤蛋白原(Fbg)ELISA试剂盒 Porcine Fibrinogen,Fbg ELISA试剂盒
猪透明质酸(HA)ELISA试剂盒 Porcine Hyaluronic acid,HA ELISA试剂盒
猪瘦素(LEP)ELISA试剂盒 Porcine Leptin,LEP ELISA试剂盒
猪生长抑素(SS)ELISA试剂盒 Porcine Somatostatin,SS ELISA试剂盒
猪生长激素(GH)ELISA试剂盒 Porcine growth hormone,GH ELISA试剂盒
猪皮质酮/肾上腺酮(CORT)ELISA试剂盒 Porcine Corticosterone,CORT ELISA试剂盒
猪皮质醇(Cortisol)ELISA试剂盒 Porcine Cortisol ELISA试剂盒
猪脑钠素/脑钠尿肽(BNP)ELISA试剂盒 Porcine brain natriuretic peptide,BNP ELISA试剂盒
猪内皮素1(ET-1)ELISA试剂盒 Porcine endothelin 1,ET-1 ELISA试剂盒
猪免疫球蛋白M(IgM)ELISA试剂盒 Porcine Immunoglobulin M,IgM ELISA试剂盒
猪免疫球蛋白G(IgG)ELISA试剂盒 Porcine Immunoglobulin G,IgG ELISA试剂盒
猪免疫球蛋白E(IgE)ELISA试剂盒 Porcine Immunoglobulin E,IgE ELISA试剂盒
猪可溶性血管细胞粘附分子1(sVCAM-1)ELISA试剂盒 Porcine soluble Vascuolar cell adhesion molecules 1,sVCAM-1 ELISA试剂盒
猪巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒 Porcine macrophage inflammatory protein 1α,MIP-1α ELISA试剂盒
猪角化细胞生长因子(KGF)ELISA试剂盒 Porcine Keratinocyte Growth Factor,KGF ELISA试剂盒
猪基质金属蛋白酶抑制因子1(TIMP-1)ELISA试剂盒 Porcine tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA试剂盒
猪基质金属蛋白酶9(MMP-9)ELISA试剂盒 Porcine matrix metalloproteinase 9,MMP-9 ELISA试剂盒
猪睾酮(T)ELISA试剂盒 Porcine Testoterone,T ELISA试剂盒
猪肝细胞生长因子(HGF)ELISA试剂盒 Porcine hepatocyte growth factor,HGF ELISA试剂盒
猪γ干扰素(IFN-γ)ELISA试剂盒 Porcine Interferon γ ,IFN-γ ELISA试剂盒
猪端粒酶(TE)ELISA试剂盒 Porcine telomerase,TE ELISA试剂盒
猪促生长激素释放激素(GHRH)ELISA试剂盒 Porcine growth hormone releasing hormone,GHRH ELISA试剂盒
猪促卵泡素(FSH)ELISA试剂盒 Porcine Follicle-stimulating hormone,FSH ELISA试剂盒
猪Ⅰ型前胶原羧基端肽(PⅠCP)ELISA试剂盒 Porcine Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA试剂盒
猪表皮生长因子(EGF)ELISA试剂盒 Porcine Epithelial growth factor,EGF ELISA试剂盒
猪白介素8(IL-8/CXCL8)ELISA试剂盒 Porcine interleukin 8,IL-8 ELISA试剂盒
猪白介素6(IL-6)ELISA试剂盒 Porcine Interleukin 6,IL-6 ELISA试剂盒
猪白介素4(IL-4)ELISA试剂盒 Porcine interleukin 4,IL-4 ELISA试剂盒
猪白介素3(IL-3)ELISA试剂盒 Porcine interleukin 3,IL-3 ELISA试剂盒
猪白介素2(IL-2)ELISA试剂盒 Porcine interleukin 2,IL-2 ELISA试剂盒
猪白介素1β (IL-1β)ELISA试剂盒 Porcine Interleukin 1β,IL-1β ELISA试剂盒
猪白介素18(IL-18)ELISA试剂盒 Porcine Interleukin 18,IL-18 ELISA试剂盒
猪白血病抑制因子受体(LIFR)ELISA试剂盒 Porcine leukemia inhibitory factor receptor,LIFR ELISA试剂盒
猪白介素10(IL-10)ELISA试剂盒 Porcine interleukin 10,IL-10 ELISA试剂盒
猪白介素1(IL-1)ELISA试剂盒 Porcine Interleukin 1,IL-1 ELISA试剂盒
猪Ⅲ型前胶原肽(PⅢNP)ELISA试剂盒 Porcine N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA试剂盒
猪Ⅲ型胶原(Col Ⅲ)ELISA试剂盒 Porcine Collagen Type Ⅲ,Col Ⅲ ELISA试剂盒
猪细胞间粘附分子2(ICAM-2/CD102)ELISA试剂盒 Porcine intercellular adhesion molecule 2,ICAM-2 ELISA试剂盒
猪细胞间粘附分子3(ICAM-3/CD50)ELISA试剂盒 Porcine intercellular adhesion molecule 3,ICAM-3 ELISA试剂盒
猪纤溶酶原激活物抑制因子1(PAI-1)ELISA试剂盒 Porcine plasminogen activator inhibitor 1,PAI-1 ELISA试剂盒
猪纤溶酶原激活物抑制因子(PAI)ELISA试剂盒 Porcine plasminogen activator inhibitor,PAI ELISA试剂盒
猪血纤蛋白原降解产物(FDP)ELISA试剂盒 Porcine Fibrinogen Degradation Product,FDP ELISA试剂盒
猪血栓调节蛋白(TM)ELISA试剂盒 Porcine thrombomodulin,TM ELISA试剂盒
猪血小板衍生生长因子(PDGF)ELISA试剂盒 Porcine platelet-derived growth factor,PDGF ELISA试剂盒
猪氧化低密度脂蛋白(OxLDL)ELISA试剂盒 Porcine oxidized lowdensity lipoprotein,OxLDL ELISA试剂盒
猪组织型纤溶酶原激活剂(t-PA)ELISA试剂盒 Porcine Tissue-type Plasiminogen Actilyse,t-PA ELISA试剂盒
猪组织因子(TF)ELISA试剂盒 Porcine Tissue factor,TF ELISA试剂盒
猪白介素1受体拮抗剂(IL1Ra)ELISA试剂盒 Porcine Interleukin 1 receptor antagonist, IL-1ra ELISA试剂盒
猪高铁血红蛋白(MHB)ELISA试剂盒 Porcine Methemoglobin,MHB ELISA试剂盒
猪Ⅲ型前胶原氨基端肽(PⅢNT)ELISA试剂盒 Porcine procollagen Ⅲ N-terminal peptide,PⅢNT ELISA试剂盒
猪低分子肝素(LMWH)ELISA试剂盒 Porcine low molecular weight heparin,LMWH ELISA试剂盒
猪单核细胞增多性李斯特菌素O((LLO)ELISA试剂盒 Porcine listeriolysin O,LLO ELISA试剂盒
鸡极低密度脂蛋白(VLDL)ELISA试剂盒 Chicken very low density lipoprotein,VLDL ELISA试剂盒
鸡转化生长因子β2(TGF-β2)ELISA试剂盒 Chicken Transforming Growth factor β2,TGF-β2 ELISA试剂盒
鸡胰高血糖素(GC)ELISA试剂盒 Chicken Glucagon,GC ELISA试剂盒
鸡甲状腺素(T4)ELISA试剂盒 Chicken Thyroxine,T4 ELISA试剂盒
鸡三碘甲状腺原氨酸(T3)ELISA试剂盒 Chicken Tri-iodothyronine,T3 ELISA试剂盒
鸡抗肌内膜抗体IgA(EMA IgA)ELISA试剂盒 Chicken anti-Endomysial Antibody IgA,EMA IgA ELISA试剂盒
鸡核因子κB(NF-κB)ELISA试剂盒 Chicken Nuclear factor-kappa B,NF-κB ELISA试剂盒
鸡弓形虫循环抗原(TCA)ELISA试剂盒 Chicken toxoplasma circulating antigen,TCA ELISA试剂盒
鸡甲状旁腺激素(PTH)ELISA试剂盒 Chicken Parathyroid hormone,PTH ELISA试剂盒
鸡卵黄免疫球蛋白(IgY)ELISA试剂盒 Hen egg Immunoglobulin of Yolk,IgY ELISA试剂盒
鸡蛋卵黄高磷蛋白磷酸肽(PPP)ELISA试剂盒 Hen egg phosvitin phosphopeptides,PPP ELISA试剂盒
鸡脂多糖/内毒素(LPS)ELISA试剂盒 Chicken Lipopolysaccharides,LPS ELISA试剂盒
鸡β干扰素(IFN-β/IFNB)ELISA试剂盒 Chicken Interferon β,IFN-β/IFNB ELISA试剂盒
鸡免疫球蛋白E(IgE)ELISA试剂盒 Chicken Immunoglobulin E,IgE ELISA试剂盒
鸡免疫球蛋白A(IgA)ELISA试剂盒 Chicken Immunoglobulin A,IgA ELISA试剂盒
鸡肿瘤坏死因子α(TNF-α)ELISA试剂盒 Chicken Tumor necrosis factor α,TNF-α ELISA试剂盒
鸡白介素1β(IL-1β)ELISA试剂盒 Chicken Interleukin 1β,IL-1β ELISA试剂盒
鸡主要组织相容性复合体(MHC/B)ELISA试剂盒 Chicken major histocompatibility complex,MHC/B ELISA试剂盒
鸡热休克蛋白70(HSP-70)ELISA试剂盒 Chicken Heat Shock Protein 70,Hsp-70 ELISA试剂盒
鸡白痢抗体检测(PD)ELISA试剂盒 Chiken Pullorum Diseaes,PD ELISA试剂盒
鸡分泌型免疫球蛋白A(SIgA)ELISA试剂盒 Chicken secretory immunoglobulin A,SIgA ELISA试剂盒
鸡α干扰素(IFN-α)ELISA试剂盒 Chicken Interferon α,IFN-α ELISA试剂盒
鸡白介素18(IL-18)ELISA试剂盒 Chicken Interleukin 18,IL-18 ELISA试剂盒
鸡白介素1(IL-1)ELISA试剂盒 Chicken Interleukin 1,IL-1 ELISA试剂盒
鸡病毒性肠炎病毒(DEV)ELISA试剂盒 Chicken virus enteritis,DEV ELISA试剂盒
鸡肌红蛋白(MYO/MB) ELISA试剂盒 Chicken Myoglobin,MYO/MB ELISA试剂盒
鸡白介素9(IL-9)ELISA试剂盒 Chicken Interleukin 9,IL-9 ELISA试剂盒
鸡白介素3(IL-3)ELISA试剂盒 Chicken Interleukin 3,IL-3 ELISA试剂盒
鸡白血病抑制因子(LIF)ELISA试剂盒 Chicken Leukemia inhibitory factor,LIF ELISA试剂盒
鸡转化生长因子β1(TGF-β1)ELISA试剂盒 Chicken Transforming Growth factor β1,TGF-β1 ELISA试剂盒
鸡C反应蛋白(CRP)ELISA试剂盒 Chicken C-Reactive Protein,CRP ELISA试剂盒
鸡血管内皮细胞生长因子(VEGF)ELISA试剂盒 Chicken Vascular Endothelial cell Growth Factor,VEGF ELISA试剂盒
鸡免疫球蛋白G(IgG)ELISA试剂盒 Chicken Immunoglobulin G,IgG ELISA试剂盒
鸡碱性成纤维细胞生长因子(bFGF)ELISA试剂盒 Chicken basic fibroblast growth factor,bFGF ELISA试剂盒
鸡可溶性细胞间粘附分子1(sICAM-1)ELISA试剂盒 Chicken Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA试剂盒
鸡可溶性E选择素(sE-selectin)ELISA试剂盒 Chicken soluble E-selectin,sE-selectin ELISA试剂盒
鸡表皮生长因子(EGF)ELISA试剂盒 Chicken Epidermal growth factor,EGF ELISA试剂盒
鸡胰岛素样生长因子1(IGF-1)ELISA试剂盒 Chicken Insulin-like growth factors 1,IGF-1 ELISA试剂盒
鸡生长激素(GH)ELISA试剂盒 Chicken growth hormone,GH ELISA试剂盒
鸡乙酰乙酸检测(ACAC)ELISA试剂盒 Chicken Acetoacetic acid,ACAC ELISA试剂盒
鸡传染性鼻炎抗体(IC)ELISA试剂盒 Chicken infectious coryza,ic ELISA试剂盒
鸡透明质酸(HA)ELISA试剂盒 Chicken Hyaluronic acid,HA ELISA试剂盒
鸡硫酸类肝素(HS)ELISA试剂盒 Chicken heparan sulfate,HS ELISA试剂盒
鸡催乳素(PRL)ELISA试剂盒 Chicken prolactin,PRL ELISA试剂盒
鸡白血病病毒抗原(ALV-AG)ELISA试剂盒 Chicken Avian Leukosis Virus AG,ALV-AG ELISA试剂盒
鸡促卵泡素(FSH)ELISA试剂盒 Chicken follicle-stimulating hormone,FSH ELISA试剂盒
鸡促黄体激素(LH)ELISA试剂盒 Chicken Luteinizing Hormone,LH ELISA试剂盒
鸡γ干扰素(IFN-γ)ELISA试剂盒 Chicken Interferon γ ,IFN-γ ELISA试剂盒
鸡白介素6(IL-6)ELISA试剂盒 Chicken Interleukin 6,IL-6 ELISA试剂盒
鸡碳酸酐酶(CA)ELISA试剂盒 Chicken carbonic anhydrase,CA ELISA试剂盒
鸡抗凝血酶Ⅲ抗体(AT-Ⅲ)ELISA试剂盒 Chicken Anti-Thrombin Ⅲ,AT-Ⅲ ELISA试剂盒
鸡热休克蛋白20(HSP-20)ELISA试剂盒 Chicken Heat Shock Protein 20,HSP-20 ELISA试剂盒
鸡热休克蛋白60(Hsp-60)ELISA试剂盒 Chicken Heat Shock Protein 60,HSP-60 ELISA试剂盒
鸡17羟皮质类固醇(17-OHCS)ELISA试剂盒 Hydroxycorticosteroids,17-OHCS ELISA试剂盒
鸡白介素4(IL-4)ELISA试剂盒 Chicken Interleukin 4,IL-4 ELISA试剂盒
鸡白介素2(IL-2)ELISA试剂盒 Chicken Interleukin 2,IL-2 ELISA试剂盒
鸡一氧化氮(NO)ELISA试剂盒 Chicken nitric oxide,NO ELISA试剂盒
鸡单纯疱疹病毒Ⅱ型抗体(HSVⅡ-Ab)ELISA试剂盒 Chicken Herpes simplexvirus Ⅱ antibody,HSVⅡ-Ab ELISA试剂盒
鸡补体3裂解产物(C3SP)ELISA试剂盒 Chicken Complement 3 split product,C3SP ELISA试剂盒
鸡肾上腺髓质素(ADM)ELISA试剂盒 Chicken adrenomedullin,ADM ELISA试剂盒
鸡巨噬细胞替代激活相关化学因子1(AmAC-1)ELISA试剂盒 Chicken Alternative macrophage activation-associated CC chemokine 1,AmAC-1 ELISA试剂盒
鸡免疫核糖核酸(Irna)ELISA试剂盒 Chicken Immune RNA,Irna ELISA试剂盒
鸡β内酰胺酶(β-lactamase)ELISA试剂盒 Chicken β-lactamase ELISA试剂盒
鸡流行性乙型脑炎抗体IgG(JE IgG)ELISA试剂盒 Chicken Japanese Encephalitis IgG,JE IgG ELISA试剂盒
鸡α葡萄糖苷酶(a-Glu)ELISA试剂盒 Chicken α-Glucosidase,a-Glu ELISA试剂盒
鸡血小板因子4(PF-4/CXCL4)ELISA试剂盒 Chicken Platelet Factor 4,PF-4 ELISA试剂盒
鸡肿瘤坏死因子相关凋亡诱导配体3(TRAIL-R3)ELISA试剂盒 Chicken tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA试剂盒
鸡L苯丙氨酸解氨酶(PAL)ELISA试剂盒 Chicken L-Phenylalanine ammonla-lyase,PAL ELISA试剂盒
鸡烟酰胺腺嘌呤二核苷酸磷酸(NADPH)ELISA试剂盒 Chicken nicotinamide adenine dinucleotide phosphate,NADPH ELISA试剂盒
鸡B因子(BF)ELISA试剂盒 Chicken factor B,BF ELISA试剂盒
鸡5核苷酸酶(5-NT)ELISA试剂盒 Chicken 5-Nucleotidase,5-NT ELISA试剂盒
鸡层连蛋白/板层素(LN)ELISA试剂盒 Chicken Laminin,LN ELISA试剂盒
鸡17-酮类固醇(17-KS)ELISA试剂盒 Chicken 17-ketosteroids,17-KS ELISA试剂盒
鸡N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒 Chicken N-acetyl-β-D-glucosaminidase,NAG ELISA试剂盒
鸡神经胶质纤维酸性蛋白(GFAP)ELISA试剂盒 Chicken glial fibrillary acidic protein,GFAP ELISA试剂盒
鸡白介素17(IL-17)ELISA试剂盒 Chicken Interleukin 17,IL-17 ELISA试剂盒
鸡1,3-βD葡葡糖苷酶(1,3-βD-Glu)ELISA试剂盒 Chicken 1,3-βD glucosidase,1,3-βD-Glu ELISA试剂盒
鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒 Chicken soluble endothelial protein C receptor,sEPCR ELISA试剂盒
禽脑脊髓炎(AE)ELISA试剂盒 Avian encephalomyelitis,AE ELISA试剂盒
鸭白介素8(IL-8/CXCL8)ELISA试剂盒 Duck Interleukin-8,IL-8 ELISA试剂盒
鸭白介素4(IL-4)ELISA试剂盒 Duck Interleukin-4,IL-4 ELISA试剂盒
鸭白介素2(IL-2)ELISA试剂盒 Duck Interleukin 2,IL-2 ELISA试剂盒
鸭子碳酸酐酶(CA)ELISA试剂盒 Duck carbonic anhydrase,CA ELISA试剂盒
鸭抗凝血素抗体(aPT1/aPT2)ELISA试剂盒 Duck anti-prothrombins antibodies,aPT1/aPT2 ELISA试剂盒
鸭环磷酸腺苷(cAMP)ELISA试剂盒 Duck cyclic adenosine monophosphate,cAMP ELISA试剂盒
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