抗利尿激素(ADH)检测试剂盒适用生物     Homo sapiens (Human，人)    抗利尿激素(ADH)检测试剂盒   检测范围     12.35-1000pg/mL     灵敏度     4.71pg/mL    样本类型     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    实验时长     2.5h     实验方法     竞争抑制法     抗利尿激素(ADH)检测试剂盒    规格     96T    ELISA Kit for Antidiuretic Hormone (ADH)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     CEB139Hu    Sample type     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    Format     96-well strip plate    Assay length     2.5 hours    Detection range     12.35-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 4.71pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Antidiuretic Hormone (ADH). No significant cross-reactivity or interference between Antidiuretic Hormone (ADH) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Antidiuretic Hormone (ADH) and the recovery rates were calculated by comparing the measured value to the expected amount of Antidiuretic Hormone (ADH) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     88-97     94    EDTA plasma(n=5)     80-90     86    heparin plasma(n=5)     91-101     96    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV抗利尿激素(ADH)检测试剂盒LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Antidiuretic Hormone (ADH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     78-101%     94-103%     78-90%     86-93%    EDTA plasma(n=5)     83-91%     79-101%     79-93%     82-92%    heparin plasma(n=5)     78-90%     95-102%     90-97%     93-105%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    Reagent Diluent     1×300μL     Stop Solution     1×6mL    TMB Substrate     1×9mL     Instruction manual     1    Wash Buffer (30 × concentrate)     1×20mL    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 50μL standard or sample to each well.    And then add 50μL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37oC;3. Aspirate and wash 3 times;4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;5. Aspirate and wash 5 times;6. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;7. Add 50μL Stop Solution. Read at 450 nm immediately.抗利尿激素(ADH)检测试剂盒Test principleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Antidiuretic Hormone (ADH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Antidiuretic Hormone (ADH) and unlabeled Antidiuretic Hormone (ADH) (Standards or samples) with the pre-coated antibody specific to Antidiuretic Hormone (ADH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Antidiuretic Hormone (ADH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Antidiuretic Hormone (ADH) in the sample.

连环蛋白β1(CTNNβ1)检测试剂盒适用生物     Homo sapiens (Human，人)    连环蛋白β1(CTNNβ1)检测试剂盒   检测范围     15.63-1000pg/mL     灵敏度     6.0pg/mL    样本类型     Serum, plasma and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     连环蛋白β1(CTNNβ1)检测试剂盒 规格     96T    连环蛋白β1(CTNNβ1)检测试剂盒   ELISA Kit for Catenin Beta 1 (CTNNb1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEB021Hu    Sample type     Serum, plasma and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 6.0pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Catenin Beta 1 (CTNNb1). No significant cross-reactivity or interference between Catenin Beta 1 (CTNNb1) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Catenin Beta 1 (CTNNb1) and the recovery rates were calculated by comparing the measured value to the expected amount of Catenin Beta 1 (CTNNb1) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     85-95     92    EDTA plasma(n=5)     86-94     91    heparin plasma(n=5)     78-99     94    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Catenin Beta 1 (CTNNb1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     94-102%     95-104%     83-99%     83-92%    EDTA plasma(n=5)     97-104%     87-95%     90-104%     86-94%    heparin plasma(n=5)     84-98%     80-103%     90-104%     95-102%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catenin Beta 1 (CTNNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catenin Beta 1 (CTNNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catenin Beta 1 (CTNNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catenin Beta 1 (CTNNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

淀粉样前体蛋白(APP)检测试剂盒适用生物     Homo sapiens (Human，人)    淀粉样前体蛋白(APP)检测试剂盒   检测范围     78.13-5000pg/mL     灵敏度     33pg/mL    样本类型     Serum, plasma, cerebrospinal fluid and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     淀粉样前体蛋白(APP)检测试剂盒  规格     96T    淀粉样前体蛋白(APP)检测试剂盒ELISA Kit for Amyloid Precursor Protein (APP)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEB020Hu    Sample type     Serum, plasma, cerebrospinal fluid and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     78.13-5000pg/mL The standard curve concentrations used for the ELISA’s were 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312.5pg/mL, 156.25pg/mL, 78.13pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 33pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Amyloid Precursor Protein (APP). No significant cross-reactivity or interference between Amyloid Precursor Protein (APP) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Amyloid Precursor Protein (APP) and the recovery rates were calculated by comparing the measured value to the expected amount of Amyloid Precursor Protein (APP) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     79-98     94    EDTA plasma(n=5)     78-99     90    heparin plasma(n=5)     94-101     97    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amyloid Precursor Protein (APP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Precursor Protein (APP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amyloid Precursor Protein (APP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     93-101%     87-101%     79-102%     78-92%    EDTA plasma(n=5)     78-96%     80-97%     81-89%     89-104%    heparin plasma(n=5)     93-103%     91-102%     82-101%     93-101%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Precursor Protein (APP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amyloid Precursor Protein (APP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amyloid Precursor Protein (APP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amyloid Precursor Protein (APP) in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

胱天蛋白酶8(CASP8)检测试剂盒适用生物 Rattus norvegicus (Rat，大鼠) 检测范围 0.313-20ng/mL 灵敏度 0.117ng/mL 样本类型 Tissue homogenates, cell lysates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 胱天蛋白酶8(CASP8)检测试剂盒ELISA Kit for Caspase 8 (CASP8)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Rattus norvegicus (Rat)    Product No.     SEA853Ra    Sample type     Tissue homogenates, cell lysates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.117ng/mL.    胱天蛋白酶8(CASP8)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Caspase 8 (CASP8). No significant cross-reactivity or interference between Caspase 8 (CASP8) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 8 (CASP8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 8 (CASP8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV胱天蛋白酶8(CASP8)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 胱天蛋白酶8(CASP8)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 8 (CASP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 8 (CASP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 8 (CASP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 8 (CASP8) in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

丙酮酸脱氢酶磷酸酶(PDP)检测试剂盒适用生物 Homo sapiens (Human，人) 检测范围 0.313-20ng/mL 灵敏度 0.125ng/mL 样本类型 Tissue homogenates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 丙酮酸脱氢酶磷酸酶(PDP)检测试剂盒ELISA Kit for Pyruvate Dehydrogenase Phosphatase (PDP)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEA887Hu    Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.125ng/mL.    丙酮酸脱氢酶磷酸酶(PDP)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Phosphatase (PDP). No significant cross-reactivity or interference between Pyruvate Dehydrogenase Phosphatase (PDP) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Dehydrogenase Phosphatase (PDP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Dehydrogenase Phosphatase (PDP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV丙酮酸脱氢酶磷酸酶(PDP)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 丙酮酸脱氢酶磷酸酶(PDP)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Phosphatase (PDP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Phosphatase (PDP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Phosphatase (PDP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Phosphatase (PDP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

甲状旁腺激素相关蛋白(PTHrP)检测试剂盒适用生物 Mus musculus (Mouse，小鼠) 检测范围 15.63-1000pg/mL 灵敏度 6.6pg/mL 样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒ELISA Kit for Parathyroid Hormone Related Protein (PTHrP)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Mus musculus (Mouse)    Product No.     SEA819Mu    Sample type     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 6.6pg/mL.    甲状旁腺激素相关蛋白(PTHrP)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone Related Protein (PTHrP). No significant cross-reactivity or interference between Parathyroid Hormone Related Protein (PTHrP) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Parathyroid Hormone Related Protein (PTHrP) and the recovery rates were calculated by comparing the measured value to the expected amount of Parathyroid Hormone Related Protein (PTHrP) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     89-97     92    EDTA plasma(n=5)     85-98     91    heparin plasma(n=5)     88-101     92    甲状旁腺激素相关蛋白(PTHrP)检测试剂盒PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone Related Protein (PTHrP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone Related Protein (PTHrP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Parathyroid Hormone Related Protein (PTHrP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     80-102%     79-93%     95-102%     84-99%    EDTA plasma(n=5)     85-99%     88-104%     85-99%     88-96%    heparin plasma(n=5)     92-103%     92-104%     93-105%     78-101%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parathyroid Hormone Related Protein (PTHrP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Parathyroid Hormone Related Protein (PTHrP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Parathyroid Hormone Related Protein (PTHrP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Parathyroid Hormone Related Protein (PTHrP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

90kDa热休克蛋白β1(HSP90β1)检测试剂盒适用生物Homo sapiens (Human，人)检测范围0.313-20ng/mL灵敏度0.117ng/mL样本类型Serum, plasma, tissue homogenates and other biological fluids.实验时长4.5h实验方法双抗夹心法   规格96TELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEA823Hu    Sample type     Serum, plasma, tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.117ng/mL.    90kDa热休克蛋白β1(HSP90β1)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90kDa Beta 1 (HSP90b1). No significant cross-reactivity or interference between Heat Shock Protein 90kDa Beta 1 (HSP90b1) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Heat Shock Protein 90kDa Beta 1 (HSP90b1) and the recovery rates were calculated by comparing the measured value to the expected amount of Heat Shock Protein 90kDa Beta 1 (HSP90b1) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     83-102     92    EDTA plasma(n=5)     84-91     88    heparin plasma(n=5)     99-105     102    Precision90kDa热休克蛋白β1(HSP90β1)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 90kDa Beta 1 (HSP90b1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 90kDa Beta 1 (HSP90b1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heat Shock Protein 90kDa Beta 1 (HSP90b1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     90-98%     80-105%     88-102%     79-103%    EDTA plasma(n=5)     87-101%     88-103%     88-96%     79-90%    heparin plasma(n=5)     79-104%     84-105%     90-98%     85-97%    90kDa热休克蛋白β1(HSP90β1)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Beta 1 (HSP90b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90kDa Beta 1 (HSP90b1) in the samples is then determined by comparing the O.D. 90kDa热休克蛋白β1(HSP90β1)检测试剂盒of the samples to the standard curve.

2015上海信裕五一放假通知                                尊敬的客户，公司各职员：        您们好！感谢大家长期关注上海信裕生物动态，根据国家法定假期的规定，并结合公司实际情况，现对五一节放假做如下安排：一、放假时间5月1日至3日(星期五至星期天)放假，共3天。其中，5月1日(星期五)为五一假期，5月2日(星期六)，5月3日(星期天)为法定节假日照常公休。二、请公司各职员做好自己的节前工作安排。三、放假期间本公司不安排人员值班，如有订购产品请发送留言至我司网站平台，我们会在节后上班第一时间及时为您办理订货发货。各网站均有在线留言咨询栏。对此给您带来的不便我们深感抱歉。感谢你的支持与理解。                                                              上海信裕 特此通知! 

Bcl2关联X蛋白(Bax)检测试剂盒适用生物 Homo sapiens (Human，人) 检测范围 0.781-50ng/mL 灵敏度 0.25ng/mL 样本类型 Serum, plasma, tissue homogenates, cell lysates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T   Bcl2关联X蛋白(Bax)检测试剂盒 ELISA Kit for Bcl2 Associated X Protein (Bax)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species Homo sapiens (Human) Product No. SEB343Hu Sample type Serum, plasma, tissue homogenates, cell lysates and other biological fluids. Format 96-well strip plate Assay length 4.5 hours Detection range 0.781-50ng/mL The standard curve concentrations used for the ELISA’s were 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL, 0.781ng/mL  Sensitivity The minimum detectable dose of this kit is typically less than 0.25ng/mL. Bcl2关联X蛋白(Bax)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Bcl2 Associated X Protein (Bax). No significant cross-reactivity or interference between Bcl2 Associated X Protein (Bax) and analogues was observed. RecoveryMatrices listed below were spiked with certain level of recombinant Bcl2 Associated X Protein (Bax) and the recovery rates were calculated by comparing the measured value to the expected amount of Bcl2 Associated X Protein (Bax) in samples. Matrix Recovery range (%) Average(%) serum(n=5) 93-105 96 EDTA plasma(n=5) 86-94 90 heparin plasma(n=5) 79-92 86 PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVBcl2关联X蛋白(Bax)检测试剂盒LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bcl2 Associated X Protein (Bax) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 83-92% 80-96% 78-92% 99-105% EDTA plasma(n=5) 94-102% 80-98% 85-99% 94-101% heparin plasma(n=5) 91-103% 90-97% 88-95% 93-104% StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Reagents and materials providedReagents Quantity Reagents Quantity Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4 Standard 2 Standard Diluent 1×20mL Detection Reagent A 1×120μL Assay Diluent A 1×12mL Detection Reagent B 1×120μL Assay Diluent B 1×12mL TMB Substrate 1×9mL Stop Solution 1×6mL Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1 Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein (Bax). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Associated X Protein (Bax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein (Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated X Protein (Bax) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

胱天蛋白酶9(CASP9)检测试剂盒适用生物 Homo sapiens (Human，人)  检测范围 0.313-20ng/mL 灵敏度 0.115ng/mL 样本类型 Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 胱天蛋白酶9(CASP9)检测试剂盒ELISA Kit for Caspase 9 (CASP9)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species Homo sapiens (Human) Product No. SEA627Hu Sample type Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Format 96-well strip plate Assay length 4.5 hours Detection range 0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL  Sensitivity The minimum detectable dose of this kit is typically less than 0.115ng/mL.胱天蛋白酶9(CASP9)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Caspase 9 (CASP9). No significant cross-reactivity or interference between Caspase 9 (CASP9) and analogues was observed. PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 9 (CASP9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 9 (CASP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV胱天蛋白酶9(CASP9)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Reagents and materials providedReagents Quantity Reagents Quantity Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4 Standard 2 Standard Diluent 1×20mL Detection Reagent A 1×120μL Assay Diluent A 1×12mL Detection Reagent B 1×120μL Assay Diluent B 1×12mL TMB Substrate 1×9mL Stop Solution 1×6mL Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1 Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 9 (CASP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 9 (CASP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 9 (CASP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 9 (CASP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

胱天蛋白酶9(CASP9)检测试剂盒适用生物 Homo sapiens (Human，人)  检测范围 0.313-20ng/mL 灵敏度 0.115ng/mL 样本类型 Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T 胱天蛋白酶9(CASP9)检测试剂盒ELISA Kit for Caspase 9 (CASP9)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species Homo sapiens (Human) Product No. SEA627Hu Sample type Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Format 96-well strip plate Assay length 4.5 hours Detection range 0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL  Sensitivity The minimum detectable dose of this kit is typically less than 0.115ng/mL.胱天蛋白酶9(CASP9)检测试剂盒SpecificityThis assay has high sensitivity and excellent specificity for detection of Caspase 9 (CASP9). No significant cross-reactivity or interference between Caspase 9 (CASP9) and analogues was observed. PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 9 (CASP9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 9 (CASP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CV胱天蛋白酶9(CASP9)检测试剂盒StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Reagents and materials providedReagents Quantity Reagents Quantity Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4 Standard 2 Standard Diluent 1×20mL Detection Reagent A 1×120μL Assay Diluent A 1×12mL Detection Reagent B 1×120μL Assay Diluent B 1×12mL TMB Substrate 1×9mL Stop Solution 1×6mL Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1 Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 9 (CASP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 9 (CASP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 9 (CASP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 9 (CASP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

周期素依赖性激酶抑制因子2A(CDKN2A)检测试剂盒适用生物 Homo sapiens (Human，人)  检测范围 0.625-40ng/mL 灵敏度 0.225ng/mL 样本类型 Tissue homogenates, cell lysates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96TELISA Kit for Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Product No.     SEA794Hu    Sample type     Tissue homogenates, cell lysates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.625-40ng/mL The standard curve concentrations used for the ELISA’s were 40ng/mL, 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.225ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase Inhibitor 2A (CDKN2A). No significant cross-reactivity or interference between Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase Inhibitor 2A (CDKN2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

C反应蛋白(CRP)检测试剂盒适用生物 Homo sapiens (Human，人)   检测范围 62.5-4000pg/mL 灵敏度 26.3pg/mL 样本类型 Serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96TC反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法)适用生物：人使用说明书仅供体外研究使用，不用于临床诊断!C反应蛋白(CRP)检测试剂盒[预期应用]本试剂盒运用双抗体夹心ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液、尿液、脑脊液、细胞培养上清或其它相关生物液体中CRP 含量。[试剂盒内容]试剂名称数量试剂名称数量96孔板(预包被) 1 96孔板覆膜4标准品2 标准品稀释液1×20mL检测溶液A 1×120μL 检测稀释液A 1×12mL检测溶液B 1×120μL 检测稀释液B 1×12mLTMB底物1×9mL 终止液1×6mL浓洗涤液(30×) 1×20mL 使用说明书1[需自备的设备及试剂]1、450±10nm 滤光片的酶标仪(建议仪器使用前提前预热)2、单道或多道微量加液器及吸头3、稀释样品的EP 管4、蒸馏水或去离子水5、吸水纸6、盛放洗液的容器[试剂盒的储存及有效期]1、未开封的试剂盒：所有试剂均按试剂瓶标签上所示保存。请注意，收到试剂盒后请尽快将标准品、检测溶液A、检测溶液B 以及96 孔板保存于-20oC。2、使用后的试剂盒：剩余试剂仍需按照试剂瓶标签所示的温度保存，开封后的酶标板要加干燥剂后密封保存于-20oC，避免潮湿。注意：试剂盒内酶标条可拆卸，按实验需求可分多次使用；使用后的剩余试剂盒建议在首次实验后1 个月内使用完毕。产品过期时间以盒子上的标签为准，保质期内所有组分都确保是稳定的。[标本的采集与保存]1、血清：将收集于血清分离管的全血标本在室温放置2 小时或4oC 过夜，然后1000×g 离心20 分钟，取上清即可，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。2、血浆：用EDTA 或肝素作为抗凝剂采集标本，并将标本在采集后的30 分钟内于2-8oC 1000×g 离心15 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。3、组织匀浆：1） 取适量组织块，于预冷PBS（0.01mol/L, pH 7.0-7.2）中清洗去除血液，称重后备用（组织块较大需先剪碎后再匀浆）；2） 可同时选用多种匀浆方法达到较好的破碎效果：首先将组织块移入玻璃匀浆器，加入5-10mL 预冷PBS 进行充分研磨，该过程需在冰上进行（有条件实验室可选用机器匀浆）；得到的匀浆液可再利用超声破碎或反复冻融进一步处理（超声破碎过程中注意冰浴降温；反复冻融法可重复2 次）。3） 将制备好的匀浆液于5000×g 离心5 分钟，留取上清即可检测。4、细胞裂解液：1）贴壁细胞需要先用胰酶消化，离心收集细胞（悬浮细胞可直接离心收集）；2）将收集到的细胞用冷PBS 洗3 次；3）物理方法裂解细胞（可先超声破碎细胞，再反复冻融）：i 超声破碎：取适量PBS 重悬细胞，用一定功率的超声波处理细胞悬液，使细胞急剧震荡破裂。ii 反复冻融：将待破碎的细胞在-20oC 以下冰冻，室温融解，反复3 次，使细胞溶胀破碎。4）将标本于2-8oC 1500×g 离心10 分钟，收集上清备用。5、尿液：请收集清晨第一次尿液（中段尿），或24 小时尿液，2000×g 离心15 分钟后收集上清，并将标本保存于-20oC，且应避免反复冻融。6、脑脊液：请离心后收集上清，并将标本保存于-20oC，且应避免反复冻融。7、细胞培养上清或其它生物标本：请1000×g 离心20 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。注意：1、以上标本均需密封保存，4oC 保存应小于1 周，-20oC 不应超过1 个月，-80oC 不应超过2 个月。2、标本溶血会影响最后检测结果，因此溶血标本不宜进行此项检测。3、标本使用前应缓慢均衡至室温，不应加热使之融解。[试剂准备]1、使用前将所有的试剂和标本缓慢均衡至室温(18-25oC)，试剂不能直接在37oC 溶解。2、标准品(冻干品)：每瓶标准品加入标准品稀释液1mL，盖好后室温静置大约10 分钟，同时反复颠倒/搓动以助溶解，其浓度为40,000pg/mL(贮液)。先将其稀释为4,000pg/mL(标准曲线最高浓度)后，再准备7 个稀释标准品的EP 管，每个EP 管中加入500μL 的标准品稀释液，如图所示依次倍比稀释成4,000pg/mL,2,000pg/mL, 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL，标准品稀释液(0pg/mL)直接作为空白孔。为保证实验结果有效性，每次实验请使用新的标准品溶液。Tube 1 2 3 4 5 6 7 8 9pg/mL 40,000 4,000 2,000 1,000 500 250 125 62.5 03、检测溶液A 及检测溶液B：Detection A 及Detection B 在使用前请手甩几下或少时离心处理，以使管壁或瓶盖的液体沉积到管底。临用前分别以检测稀释液A 或B1:100 稀释(如：10μL 检测溶液A/990μL 检测稀释液A)，充分混匀，稀释前根据预先计算好的每次实验所需的总量配制(100μL/孔)，实际配制时应多配制0.1-0.2mL。4、浓洗涤液：用580mL 蒸馏水或去离子水将20mL 浓洗涤液稀释至600mL，进行30 倍稀释。5、底物溶液：请用灭菌的移液器吸头吸取所需体积的TMB 至另一干净容器中使用，容器中剩余的底物应予丢弃，不要倒回TMB 瓶中。注意：1、标准品的稀释不能直接在板中进行。2、标准品请于临用前15 分钟内配制。该标准品只能使用一次。3、标准品、检测溶液A 工作液、检测溶液B 工作液请使用相应的稀释液配制，不能混淆。混匀时要轻轻充分混匀，避免起泡。为保证实验结果的准确请使用微量吸管，并校准微量加液器。请依据所需的量精确配制，尽量不要微量配制(如吸取检测溶液A 时，一次不要小于10μL)，以避免造成浓度误差。4、请勿重复使用已经稀释过的标准品、检测溶液A 工作液和检测溶液B 工作液。5、浓洗涤液中如有结晶析出，请先温育至室温，轻轻混匀，至到结晶完全溶解再进行配制。6、试剂盒中部分试剂为储存液，客户需配置成工作液后使用，在配置过程中如因纯净水质量差或水质污染，以及实验中所用耗材洁净度差，可能造成实验结果不准确，甚至完全错误，请使用双蒸水。[标本处理]1、本公司只对试剂盒本身负责，不对因使用该试剂盒所造成的样本消耗负责，请使用者使用前充分考虑到样本的可能使用量，预留充足的样本。2、实验前应预测标本含量，如果标本浓度过高，应对标本进行稀释，使稀释后的标本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。3、血清或血浆标本推荐稀释大约500 倍或500 倍以上，如：稀释500 倍，首先取20μL 血清或血浆样本加入180μL PBS，做10 倍稀释，然后再取10 倍稀释后的样本10μL 加入490μL PBS 即可，稀释的每一步都需混匀。标本使用0.01mol/L 的PBS 稀释(PH=7.0-7.2）。4、若所检样本不包含在说明书所列样本之中，建议进行预实验验证其有效性，并注意留存样本。5、使用化学裂解液制备的组织匀浆或细胞提取液可能会由于某些化学物质的引入导致ELISA 实验结果偏差。6、若样本为细胞培养上清，因该类样本干扰因素较多，如：细胞状态、细胞数量、采样时间等，所以可能存在检测不出的情况。7、某些天然蛋白或重组蛋白，包括原核及真核重组蛋白，可能因为与本产品所使用的检测抗体及捕获抗体不匹配，而不被检测出。8、建议使用新鲜样本，保存时间过长可能会存在蛋白降解或变性导致实验结果偏差。[操作步骤]1、加样：分别设标准孔、待测样品孔、空白孔。设标准孔7 孔，依次加入100μL 不同浓度的标准品(见试剂准备2)。空白孔加100μL(见试剂准备第二步最后一管)，余孔加待测样品100μL，酶标板加上覆膜，37oC 温育2 小时。2、弃去液体，甩干，不用洗涤。3、每孔加检测溶液A 工作液100μL(临用前配制)，酶标板加上覆膜，37oC 温育1 小时。4、弃去孔内液体，每孔用350μL 的洗涤液洗涤，浸泡1-2 分钟，吸去(不可触及板壁)或甩掉酶标板内的液体，在实验台上铺垫几层吸水纸，酶标板朝下用力拍几次(也可轻拍将孔内液体拍干)，重复洗板3 次。最后一次洗涤后，要把孔内的洗涤液完全甩干。自动洗板机亦可。5、每孔加检测溶液B 工作液(临用前配制)100μL，加上覆膜，37oC 温育30 分钟。6、弃去孔内液体，甩干，洗板5 次，方法同步骤4。7、每孔加底物溶液90μL，酶标板加上覆膜，37oC 避光显色(反应时间控制在15-25 分钟，不要超过30 分钟。当标准孔的前3-4 孔有明显的梯度蓝色，后3-4 孔梯度不明显时，即可终止)。8、每孔加终止溶液50μL，终止反应，此时蓝色立转黄色。终止液的加入顺序应尽量与底物溶液的加入顺序相同。如出现颜色不匀一，请轻轻晃动酶标板以使溶液混合均匀。9、在确保酶标板底无水滴及孔内无气泡后，立即用酶标仪在450nm 波长测量各孔的光密度(O.D.值)。注意：1、试剂准备：准备一次实验所需要的酶标条，其它的可从微孔板上拆下，密封，按照说明书要求保存，以备下次使用。2、加样：实验操作中请使用一次性的吸头，避免交叉污染。加样时注意不要有气泡，将样品加于酶标板底部，尽量不触及孔壁，轻轻晃动混匀。加样或加试剂时，第一个孔与最后一个孔加样之间的时间间隔如果太大，将会导致不同的“预温育”时间，从而明显地影响到测量值的准确性及重复性。因此，一次加样时间(包括标准品及所有样品)最好控制在10 分钟内。推荐设置复孔进行实验。3、温育：为防止样品蒸发，实验时请将加上盖或覆膜的酶标板置于湿盒内，以避免液体蒸发，洗板后应尽快进行下步操作，任何时侯都应避免酶标板处于干燥状态，同时应严格遵守给定的温育时间和温度。4、洗涤：充分的洗涤非常重要，在每次洗涤过程中，都要将洗涤液完全甩干。洗涤过程中反应孔中残留的洗涤液应在滤纸上拍干，勿将滤纸直接放入反应孔中吸水，同时要消除板底残留的液体和手指印，避免影响最后的酶标仪读数。如果有自动洗板机，应在熟练使用后再用到正式实验过程中。5、反应时间的控制：加入底物后请定时观察反应孔的颜色变化(比如，每隔10 分钟观察一次)，如颜色较深，请提前加入终止液终止反应，避免反应过强从而影响酶标仪光密度读数。6、底物：底物请避光保存，在储存和温育时避免强光直接照射。7、如果实验室内湿度低于60%，推荐使用加湿器提高湿度水平。[实验原理]将CRP 抗体包被于96 孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的CRP 与连接于固相载体上的抗体结合，然后加入生物素化的CRP 抗体，将未结合的生物素化抗体洗净后，加入HRP 标记的亲和素，再次彻底洗涤后加入TMB 底物显色。TMB 在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CRP 呈正相关。用酶标仪在450nm 波长下测定吸光度(O.D.值)，计算样品浓度。[计算]各标准品及样本O.D.值扣除空白孔O.D.值后作图(七点图)，如设置复孔，则应取其平均值计算。以标准品的浓度为纵坐标(或对数坐标)，O.D.值为横坐标(或对数坐标），绘出标准曲线(最佳方程式应依回归方程计算的R2值来定，以R2 值越趋近于1 为好)。推荐使用专业制作曲线软件进行分析，如curve expert 1.30，根据样品O.D.值，由标准曲线查出相应的浓度，乘以稀释倍数；或用标准物的浓度与O.D.值计算出标准曲

脂蛋白脂酶(LIPD)检测试剂盒适用生物 Homo sapiens (Human，人)  检测范围 0.625-40ng/mL 灵敏度 0.247ng/mL 样本类型 Serum, plasma, tissue homogenates, cell lysates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T  ELISA Kit for Lipase, Lipoprotein (LIPD)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism speciesHomo sapiens (Human)Product No.SEA386HuSample typeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.Format96-well strip plateAssay length4.5 hoursDetection range0.625-40ng/mL The standard curve concentrations used for the ELISA’s were 40ng/mL, 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mLSensitivityThe minimum detectable dose of this kit is typically less than 0.247ng/mL.SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipase, Lipoprotein (LIPD). No significant cross-reactivity or interference between Lipase, Lipoprotein (LIPD) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Lipase, Lipoprotein (LIPD) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipase, Lipoprotein (LIPD) in samples. MatrixRecovery range (%)Average(%)serum(n=5)89-10294EDTA plasma(n=5)83-105101heparin plasma(n=5)95-10299PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Lipoprotein (LIPD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Lipoprotein (LIPD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipase, Lipoprotein (LIPD) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample1:21:41:81:16serum(n=5)94-102%99-105%85-92%96-103%EDTA plasma(n=5)79-101%79-96%83-93%78-92%heparin plasma(n=5)78-93%86-93%84-91%87-101%StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagentsQuantityReagentsQuantityPre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4Standard2Standard Diluent1×20mLDetection Reagent A1×120μLAssay Diluent A1×12mLDetection Reagent B1×120μLAssay Diluent B1×12mLTMB Substrate1×9mLStop Solution1×6mLWash Buffer (30 × concentrate)1×20mLInstruction manual1Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Lipoprotein (LIPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Lipoprotein (LIPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Lipoprotein (LIPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Lipoprotein (LIPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.     ELISA Kit for Lipase, Lipoprotein (LIPD)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism speciesHomo sapiens (Human)Product No.SEA386HuSample typeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.Format96-well strip plateAssay length4.5 hoursDetection range0.625-40ng/mL The standard curve concentrations used for the ELISA’s were 40ng/mL, 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mLSensitivityThe minimum detectable dose of this kit is typically less than 0.247ng/mL.SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipase, Lipoprotein (LIPD). No significant cross-reactivity or interference between Lipase, Lipoprotein (LIPD) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Lipase, Lipoprotein (LIPD) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipase, Lipoprotein (LIPD) in samples. MatrixRecovery range (%)Average(%)serum(n=5)89-10294EDTA plasma(n=5)83-105101heparin plasma(n=5)95-10299PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Lipoprotein (LIPD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Lipoprotein (LIPD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipase, Lipoprotein (LIPD) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample1:21:41:81:16serum(n=5)94-102%99-105%85-92%96-103%EDTA plasma(n=5)79-101%79-96%83-93%78-92%heparin plasma(n=5)78-93%86-93%84-91%87-101%StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagentsQuantityReagentsQuantityPre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4Standard2Standard Diluent1×20mLDetection Reagent A1×120μLAssay Diluent A1×12mLDetection Reagent B1×120μLAssay Diluent B1×12mLTMB Substrate1×9mLStop Solution1×6mLWash Buffer (30 × concentrate)1×20mLInstruction manual1Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Lipoprotein (LIPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Lipoprotein (LIPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Lipoprotein (LIPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Lipoprotein (LIPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.ELISA Kit for Lipase, Lipoprotein (LIPD)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism speciesHomo sapiens (Human)Product No.SEA386HuSample typeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.Format96-well strip plateAssay length4.5 hoursDetection range0.625-40ng/mL The standard curve concentrations used for the ELISA’s were 40ng/mL, 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mLSensitivityThe minimum detectable dose of this kit is typically less than 0.247ng/mL.SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipase, Lipoprotein (LIPD). No significant cross-reactivity or interference between Lipase, Lipoprotein (LIPD) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Lipase, Lipoprotein (LIPD) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipase, Lipoprotein (LIPD) in samples. MatrixRecovery range (%)Average(%)serum(n=5)89-10294EDTA plasma(n=5)83-105101heparin plasma(n=5)95-10299PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Lipoprotein (LIPD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Lipoprotein (LIPD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipase, Lipoprotein (LIPD) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample1:21:41:81:16serum(n=5)94-102%99-105%85-92%96-103%EDTA plasma(n=5)79-101%79-96%83-93%78-92%heparin plasma(n=5)78-93%86-93%84-91%87-101%StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagentsQuantityReagentsQuantityPre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4Standard2Standard Diluent1×20mLDetection Reagent A1×120μLAssay Diluent A1×12mLDetection Reagent B1×120μLAssay Diluent B1×12mLTMB Substrate1×9mLStop Solution1×6mLWash Buffer (30 × concentrate)1×20mLInstruction manual1Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Lipoprotein (LIPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Lipoprotein (LIPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Lipoprotein (LIPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Lipoprotein (LIPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒适用生物 Homo sapiens (Human，人) 检测范围 0.156-10ng/mL 灵敏度 0.055ng/mL 样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法 规格 96T 趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒(酶联免疫吸附试验法)适用生物：人使用说明书仅供体外研究使用，不用于临床诊断!趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒[预期应用]本试剂盒运用双抗体夹心ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液、细胞培养上清或其它相关生物液体中CX3CL1 含量。[试剂盒内容]试剂名称数量试剂名称数量96孔板(预包被) 1 96孔板覆膜4标准品2 标准品稀释液1×20mL检测溶液A 1×120μL 检测稀释液A 1×12mL检测溶液B 1×120μL 检测稀释液B 1×12mLTMB底物1×9mL 终止液1×6mL浓洗涤液(30×) 1×20mL 使用说明书1[需自备的设备及试剂]1、450±10nm 滤光片的酶标仪(建议仪器使用前提前预热)2、单道或多道微量加液器及吸头3、稀释样品的EP 管4、蒸馏水或去离子水5、吸水纸6、盛放洗液的容器[试剂盒的储存及有效期]1、未开封的试剂盒：所有试剂均按试剂瓶标签上所示保存。请注意，收到试剂盒后请尽快将标准品、检测溶液A、检测溶液B 以及96 孔板保存于-20oC。2、使用后的试剂盒：剩余试剂仍需按照试剂瓶标签所示的温度保存，开封后的酶标板要加干燥剂后密封保存于-20oC，避免潮湿。注意：试剂盒内酶标条可拆卸，按实验需求可分多次使用；使用后的剩余试剂盒建议在首次实验后1 个月内使用完毕。产品过期时间以盒子上的标签为准，保质期内所有组分都确保是稳定的。[标本的采集与保存]1、血清：将收集于血清分离管的全血标本在室温放置2 小时或4oC 过夜，然后1000×g 离心20 分钟，取上清即可，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。2、血浆：用EDTA 或肝素作为抗凝剂采集标本，并将标本在采集后的30 分钟内于2-8oC 1000×g 离心15 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。3、组织匀浆：1） 取适量组织块，于预冷PBS（0.01mol/L, pH 7.0-7.2）中清洗去除血液，称重后备用（组织块较大需先剪碎后再匀浆）；2） 可同时选用多种匀浆方法达到较好的破碎效果：首先将组织块移入玻璃匀浆器，加入5-10mL 预冷PBS 进行充分研磨，该过程需在冰上进行（有条件实验室可选用机器匀浆）；得到的匀浆液可再利用超声破碎或反复冻融进一步处理（超声破碎过程中注意冰浴降温；反复冻融法可重复2 次）。3） 将制备好的匀浆液于5000×g 离心5 分钟，留取上清即可检测。4、细胞裂解液：1）贴壁细胞需要先用胰酶消化，离心收集细胞（悬浮细胞可直接离心收集）；2）将收集到的细胞用冷PBS 洗3 次；3）物理方法裂解细胞（可先超声破碎细胞，再反复冻融）：i 超声破碎：取适量PBS 重悬细胞，用一定功率的超声波处理细胞悬液，使细胞急剧震荡破裂。ii 反复冻融：将待破碎的细胞在-20oC 以下冰冻，室温融解，反复3 次，使细胞溶胀破碎。4）将标本于2-8oC 1500×g 离心10 分钟，收集上清备用。5、细胞培养上清或其它生物标本：请1000×g 离心20 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。注意：1、以上标本均需密封保存，4oC 保存应小于1 周，-20oC 不应超过1 个月，-80oC 不应超过2 个月。2、标本溶血会影响最后检测结果，因此溶血标本不宜进行此项检测。3、标本使用前应缓慢均衡至室温，不应加热使之融解。[试剂准备]1、使用前将所有的试剂和标本缓慢均衡至室温(18-25oC)，试剂不能直接在37oC 溶解。2、标准品(冻干品)：每瓶标准品加入标准品稀释液1mL，盖好后室温静置大约10 分钟，同时反复颠倒/搓动以助溶解，其浓度为40ng/mL(贮液)。先将其稀释为10ng/mL(标准曲线最高浓度)后，再准备7 个稀释标准品的EP 管，每个EP 管中加入500μL 的标准品稀释液，如图所示依次倍比稀释成10ng/mL，5ng/mL，2.5ng/mL，1.25ng/mL，0.625ng/mL，0.312ng/mL，0.156ng/mL，标准品稀释液(0ng/mL)直接作为空白孔。为保证实验结果有效性，每次实验请使用新的标准品溶液。Tube 1 2 3 4 5 6 7 8 9ng/mL 40 10 5 2.5 1.25 0.625 0.312 0.156 03、检测溶液A 及检测溶液B：Detection A 及Detection B 在使用前请手甩几下或少时离心处理，以使管壁或瓶盖的液体沉积到管底。临用前分别以检测稀释液A 或B1:100 稀释(如：10μL 检测溶液A/990μL 检测稀释液A)，充分混匀，稀释前根据预先计算好的每次实验所需的总量配制(100μL/孔)，实际配制时应多配制0.1-0.2mL。4、浓洗涤液：用580mL 蒸馏水或去离子水将20mL 浓洗涤液稀释至600mL，进行30 倍稀释。5、底物溶液：请用灭菌的移液器吸头吸取所需体积的TMB 至另一干净容器中使用，容器中剩余的底物应予丢弃，不要倒回TMB 瓶中。注意：1、标准品的稀释不能直接在板中进行。2、标准品请于临用前15 分钟内配制。该标准品只能使用一次。3、标准品、检测溶液A 工作液、检测溶液B 工作液请使用相应的稀释液配制，不能混淆。混匀时要轻轻充分混匀，避免起泡。为保证实验结果的准确请使用微量吸管，并校准微量加液器。请依据所需的量精确配制，尽量不要微量配制(如吸取检测溶液A 时，一次不要小于10μL)，以避免造成浓度误差。4、请勿重复使用已经稀释过的标准品、检测溶液A 工作液和检测溶液B 工作液。5、浓洗涤液中如有结晶析出，请先温育至室温，轻轻混匀，至到结晶完全溶解再进行配制。6、试剂盒中部分试剂为储存液，客户需配置成工作液后使用，在配置过程中如因纯净水质量差或水质污染，以及实验中所用耗材洁净度差，可能造成实验结果不准确，甚至完全错误，请使用双蒸水。[标本处理]1、本公司只对试剂盒本身负责，不对因使用该试剂盒所造成的样本消耗负责，请使用者使用前充分考虑到样本的可能使用量，预留充足的样本。2、实验前应预测标本含量，如果标本浓度过高，应对标本进行稀释，使稀释后的标本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。标本使用0.01mol/L 的PBS 稀释(PH=7.0-7.2)。3、若所检样本不包含在说明书所列样本之中，建议进行预实验验证其有效性，并注意留存样本。4、使用化学裂解液制备的组织匀浆或细胞提取液可能会由于某些化学物质的引入导致ELISA 实验结果偏差。5、若样本为细胞培养上清，因该类样本干扰因素较多，如：细胞状态、细胞数量、采样时间等，所以可能存在检测不出的情况。6、某些天然蛋白或重组蛋白，包括原核及真核重组蛋白，可能因为与本产品所使用的检测抗体及捕获抗体不匹配，而不被检测出。7、建议使用新鲜样本，保存时间过长可能会存在蛋白降解或变性导致实验结果偏差。[操作步骤]1、加样：分别设标准孔、待测样品孔、空白孔。设标准孔7 孔，依次加入100μL 不同浓度的标准品(见试剂准备2)。空白孔加100μL(见试剂准备第二步最后一管)，余孔加待测样品100μL，酶标板加上覆膜，37oC 温育2 小时。2、弃去液体，甩干，不用洗涤。3、每孔加检测溶液A 工作液100μL(临用前配制)，酶标板加上覆膜，37oC 温育1 小时。4、弃去孔内液体，每孔用350μL 的洗涤液洗涤，浸泡1-2 分钟，吸去(不可触及板壁)或甩掉酶标板内的液体，在实验台上铺垫几层吸水纸，酶标板朝下用力拍几次(也可轻拍将孔内液体拍干)，重复洗板3 次。最后一次洗涤后，要把孔内的洗涤液完全甩干。自动洗板机亦可。5、每孔加检测溶液B 工作液(临用前配制)100μL，加上覆膜，37oC 温育30 分钟。6、弃去孔内液体，甩干，洗板5 次，方法同步骤4。7、每孔加底物溶液90μL，酶标板加上覆膜，37oC 避光显色(反应时间控制在15-25 分钟，不要超过30 分钟。当标准孔的前3-4 孔有明显的梯度蓝色，后3-4 孔梯度不明显时，即可终止)。8、每孔加终止溶液50μL，终止反应，此时蓝色立转黄色。终止液的加入顺序应尽量与底物溶液的加入顺序相同。如出现颜色不匀一，请轻轻晃动酶标板以使溶液混合均匀。9、在确保酶标板底无水滴及孔内无气泡后，立即用酶标仪在450nm 波长测量各孔的光密度(O.D.值)。注意：1、试剂准备：准备一次实验所需要的酶标条，其它的可从微孔板上拆下，密封，按照说明书要求保存，以备下次使用。2、加样：实验操作中请使用一次性的吸头，避免交叉污染。加样时注意不要有气泡，将样品加于酶标板底部，尽量不触及孔壁，轻轻晃动混匀。加样或加试剂时，第一个孔与最后一个孔加样之间的时间间隔如果太大，将会导致不同的“预温育”时间，从而明显地影响到测量值的准确性及重复性。因此，一次加样时间(包括标准品及所有样品)最好控制在10 分钟内。推荐设置复孔进行实验。3、温育：为防止样品蒸发，实验时请将加上盖或覆膜的酶标板置于湿盒内，以避免液体蒸发，洗板后应尽快进行下步操作，任何时侯都应避免酶标板处于干燥状态，同时应严格遵守给定的温育时间和温度。4、洗涤：充分的洗涤非常重要，在每次洗涤过程中，都要将洗涤液完全甩干。洗涤过程中反应孔中残留的洗涤液应在滤纸上拍干，勿将滤纸直接放入反应孔中吸水，同时要消除板底残留的液体和手指印，避免影响最后的酶标仪读数。如果有自动洗板机，应在熟练使用后再用到正式实验过程中。5、反应时间的控制：加入底物后请定时观察反应孔的颜色变化(比如，每隔10 分钟观察一次)，如颜色较深，请提前加入终止液终止反应，避免反应过强从而影响酶标仪光密度读数。6、底物：底物请避光保存，在储存和温育时避免强光直接照射。7、如果实验室内湿度低于60%，推荐使用加湿器提高湿度水平。[实验原理]将CX3CL1 抗体包被于96 孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的CX3CL1 与连接于固相载体上的抗体结合，然后加入生物素化的CX3CL1 抗体，将未结合的生物素化抗体洗净后，加入HRP 标记的亲和素，再次彻底洗涤后加入TMB 底物显色。TMB 在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CX3CL1 呈正相关。用酶标仪在450nm 波长下测定吸光度(O.D.值)，计算样品浓度。[计算]各标准品及样本O.D.值扣除空白孔O.D.值后作图(七点图)，如设置复孔，则应取其平均值计算。以标准品的浓度为纵坐标(或对数坐标)，O.D.值为横坐标(或对数坐标），绘出标准曲线(最佳方程式应依回归方程计算的R2值来定，以R2 值越趋近于1 为好)。推荐使用专业制作曲线软件进行分析，如curve expert 1.30，根据样品O.D.值，由标准曲线查出相应的浓度，乘以稀释倍数；或用标准物的浓度与O.D.值计算出标准曲线的回归方程式，将样品的O.D.值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。[典型数据]为了便于计算，尽管浓度为自变量而O.D.值为因变量，我们绘图时仍采用标准品的O.D.值作为横坐标(X 轴)，标准品的浓度为纵坐标(Y 轴)。同时为了试验结果的直观性，图中提供的是原始数据而非对数值。推荐使用对数值建立标准曲线图。由于实验操作条件的不同（如操作者、移液技术、洗板技术和温度条件等），标准曲线的O.D.值会有所差异。所提供的标准曲线仅供参考，实验者需要根据自已的实验建立标准曲线。人趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒标准曲线[检测范围]0.156ng/mL-10ng/mL[最低检测限]0.055ng/mL此值为20 个空白样品(即标准品稀释液)测定的平均值加二倍标准差所对应的浓度。[特异性]本试剂盒用于检测CX3CL1，经检测与其它相似物质无明显交叉反应。由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。[回收率]分别于定值血清及血浆样本中加入一定量的CX3CL1（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。样本回收率范围(%) 平均回收率(%)血清(n=5) 87-98 92EDTA 血浆(n=5) 78-93 86肝素血浆(n=5) 94-104 99[线性]在定值血清及血浆样本内加入适量的CX3CL1，并倍比稀释成1:2，1:4，1:8，1:16 的待测样本，线性范围即为稀释后样本中CX3CL1 含量的测定值与理论值的比率。样本1：2 1：4 1：8 1：16血清(n=5) 96-105% 90-103% 78-91% 93-101%EDTA 血浆(n=5) 79-89% 98-105% 86-102% 81-93%肝素血浆(n=5) 89-97% 80-92% 84-104% 86-99%[精密度]精密度用样品测定值的变异系数CV 表示。CV(%) = SD/mean×100批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD 值。批间差：选取3 个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8 次，分别计算不同浓度样本的平均值及SD 值。批内差： CV批间差： CV[稳定性]经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。[实验流程]1、实验前标准品、试剂及样本的准备；2、加样（标准品及样本）100μL，37oC孵育2小时；3、吸弃，加检测溶液A100μL，37oC孵育1小时；4、洗板3次；5、加检测溶液B100μL，37oC孵育30分钟；6、洗板5次；7、加TMB底物90μL，37oC孵育15－25分钟；8、加终止液50μL，立即450nm读数。[说明]1、由于现有条件及科学技术水平尚不能对所有供货商提供的所有原料进行全面的鉴定与分析，本产品可能存在一定的质量技术风险。2、最终的实验结果与试剂的有效性、实验者的相关操作以及当时的实验环境密切相关，请务必准备充足的标本备份。3、不同批次的同一产品可能会有少许差别，如：检测限、灵敏度以及显色时间等，请依据试剂盒内说明书进行实验操作，网站电子版说明书仅作参考。4、只有全部使用本试剂盒配套试剂才能保证检测效果，不能混用其他制造商的产品。只有严格遵守本试剂盒的实验说明才会得到最佳的检测结果。5、在储存及温育过程中避免将试剂暴露在强光中。所有试剂瓶盖须盖紧以防止蒸发和微生物的污染，因为蛋白水解酶的干扰将导致出现错误的结果。6、刚开启的酶联板孔中可能会有少许水样物质，此为正常现象，不会对实验结果造成任何影响。请勿提前将酶标板从包装袋里拿出。7、由于操作者不熟练、操作失误或读数仪程序选用错误等有可能会导致错误结果的产生。请使用者使用该产品前仔细阅读说明书，调试好酶标仪，请使用配备有450±10nm 滤光片的酶标仪，且该酶标仪测量范围在0.001-3.000 O.D.或以上。8、同一使用者在使用同一产品时，如不同时间订购，也可能会因不同批次的少量差异产生不同结果，因此建议使用者在每次使用前均进行预实验。9、试剂盒在出厂前均经过严格检测，但由于运输条件及各实验室条件差异，可能会造成实验结果与出厂结果不一致或不同批次试剂盒批间差大的情况，对于这种情况会酌情处理。10、本试剂盒未与其他厂家同类试剂盒或不同方法检测同一目的蛋白的产品做对比，平行检测可能会存在检测结果不一致的情况。11、本操作说明同样适用于48T 试剂盒，但48T 试剂盒所有试剂减半。[警告]本试剂盒中使用了稀硫酸作为终止液，其具有轻微腐蚀性，使用时应避免接触衣物或眼、手等皮肤暴露部位。[问题解答]问题可能原因解决方案标准曲线差标准品准备不正确进行正确的标准品梯度稀释吸取及洗涤不充分充分的吸取及洗涤移液不精确检查和校正移液器精密度低洗涤不充分按说明书要求充分洗涤和浸泡混匀不充分和吸取试剂不足充分混匀和吸取试剂重复利用吸头、容器和覆膜使用加样器要更换新的吸头、使用新的容器和覆膜加样不精确检查和校正移液器O.D 值低每孔加的试剂量不精确校正移液器，精确加入试剂温育时间不正确保证充足的温育时间温育温度不正确试剂要平衡至室温并保证准确的温育温度酶标记物或底物失效通过混合酶标记物和底物，颜色应迅速显现来检查没有加入终止液按照说明书实验操作步骤加入终止液超出读数时间读数在说明书推荐的读数时间内读数样本值不正确的样本储存方式正确储存样本，使用新鲜样本进行实验不正确的样本收集和处理方法采取正确的样本收集和处理方法待测物质在样本中含量低使用新鲜样本，重复实验

FMS样酪氨酸激酶3配体(Flt3L)检测试剂盒适用生物 Homo sapiens (Human，人) 检测范围 31.25-2000pg/mL 灵敏度 12.5pg/mL 样本类型 Serum, plasma, tissue homogenates and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法  规格 96T FMS 样酪氨酸激酶3 配体(Flt3L)检测试剂盒(酶联免疫吸附试验法)适用生物：人使用说明书仅供体外研究使用，不用于临床诊断!FMS 样酪氨酸激酶3 配体(Flt3L)检测试剂盒[预期应用]本试剂盒运用双抗体夹心ELISA 法定量测定人血清、血浆、组织匀浆或其它相关生物液体中Flt3L 含量。[试剂盒内容]试剂名称数量试剂名称数量96孔板(预包被) 1 96孔板覆膜4标准品2 标准品稀释液1×20mL检测溶液A 1×120μL 检测稀释液A 1×12mL检测溶液B 1×120μL 检测稀释液B 1×12mLTMB底物1×9mL 终止液1×6mL浓洗涤液(30×) 1×20mL 使用说明书1[需自备的设备及试剂]1、450±10nm 滤光片的酶标仪(建议仪器使用前提前预热)2、单道或多道微量加液器及吸头3、稀释样品的EP 管4、蒸馏水或去离子水5、吸水纸6、盛放洗液的容器[试剂盒的储存及有效期]1、未开封的试剂盒：所有试剂均按试剂瓶标签上所示保存。请注意，收到试剂盒后请尽快将标准品、检测溶液A、检测溶液B 以及96 孔板保存于-20oC。2、使用后的试剂盒：剩余试剂仍需按照试剂瓶标签所示的温度保存，开封后的酶标板要加干燥剂后密封保存于-20oC，避免潮湿。注意：试剂盒内酶标条可拆卸，按实验需求可分多次使用；使用后的剩余试剂盒建议在首次实验后1 个月内使用完毕。产品过期时间以盒子上的标签为准，保质期内所有组分都确保是稳定的。[标本的采集与保存]1、血清：将收集于血清分离管的全血标本在室温放置2 小时或4oC 过夜，然后1000×g 离心20 分钟，取上清即可，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。2、血浆：用EDTA 或肝素作为抗凝剂采集标本，并将标本在采集后的30 分钟内于2-8oC 1000×g 离心15 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。3、组织匀浆：1） 取适量组织块，于预冷PBS（0.01mol/L, pH 7.0-7.2）中清洗去除血液，称重后备用（组织块较大需先剪碎后再匀浆）；2） 可同时选用多种匀浆方法达到较好的破碎效果：首先将组织块移入玻璃匀浆器，加入5-10mL 预冷PBS 进行充分研磨，该过程需在冰上进行（有条件实验室可选用机器匀浆）；得到的匀浆液可再利用超声破碎或反复冻融进一步处理（超声破碎过程中注意冰浴降温；反复冻融法可重复2 次）。3） 将制备好的匀浆液于5000×g 离心5 分钟，留取上清即可检测。4、其它生物标本：请1000×g 离心20 分钟，取上清即可检测，或将上清置于-20oC 或-80oC 保存，但应避免反复冻融。注意：1、以上标本均需密封保存，4oC 保存应小于1 周，-20oC 不应超过1 个月，-80oC 不应超过2 个月。2、标本溶血会影响最后检测结果，因此溶血标本不宜进行此项检测。3、标本使用前应缓慢均衡至室温，不应加热使之融解。[试剂准备]1、使用前将所有的试剂和标本缓慢均衡至室温(18-25oC)，试剂不能直接在37oC 溶解。2、标准品(冻干品)：每瓶标准品加入标准品稀释液1mL，盖好后室温静置大约10 分钟，同时反复颠倒/搓动以助溶解，其浓度为2,000pg/mL。准备7 个稀释标准品的EP 管，每个EP 管中加入500μL 的标准品稀释液，如图所示依次倍比稀释成2,000pg/mL，1,000pg/mL，500pg/mL，250pg/mL，125pg/mL，62.5pg/mL，31.2pg/mL，标准品稀释液(0pg/mL)直接作为空白孔。为保证实验结果有效性，每次实验请使用新的标准品溶液。Tube 1 2 3 4 5 6 7 8pg/mL 2,000 1,000 500 250 125 62.5 31.2 03、检测溶液A 及检测溶液B：Detection A 及Detection B 在使用前请手甩几下或少时离心处理，以使管壁或瓶盖的液体沉积到管底。临用前分别以检测稀释液A 或B1:100 稀释(如：10μL 检测溶液A/990μL 检测稀释液A)，充分混匀，稀释前根据预先计算好的每次实验所需的总量配制(100μL/孔)，实际配制时应多配制0.1-0.2mL。4、浓洗涤液：用580mL 蒸馏水或去离子水将20mL 浓洗涤液稀释至600mL，进行30 倍稀释。5、底物溶液：请用灭菌的移液器吸头吸取所需体积的TMB 至另一干净容器中使用，容器中剩余的底物应予丢弃，不要倒回TMB 瓶中。注意：1、标准品的稀释不能直接在板中进行。2、标准品请于临用前15 分钟内配制。该标准品只能使用一次。3、标准品、检测溶液A 工作液、检测溶液B 工作液请使用相应的稀释液配制，不能混淆。混匀时要轻轻充分混匀，避免起泡。为保证实验结果的准确请使用微量吸管，并校准微量加液器。请依据所需的量精确配制，尽量不要微量配制(如吸取检测溶液A 时，一次不要小于10μL)，以避免造成浓度误差。4、请勿重复使用已经稀释过的标准品、检测溶液A 工作液和检测溶液B 工作液。5、浓洗涤液中如有结晶析出，请先温育至室温，轻轻混匀，至到结晶完全溶解再进行配制。6、试剂盒中部分试剂为储存液，客户需配置成工作液后使用，在配置过程中如因纯净水质量差或水质污染，以及实验中所用耗材洁净度差，可能造成实验结果不准确，甚至完全错误，请使用双蒸水。[标本处理]1、本公司只对试剂盒本身负责，不对因使用该试剂盒所造成的样本消耗负责，请使用者使用前充分考虑到样本的可能使用量，预留充足的样本。2、实验前应预测标本含量，如果标本浓度过高，应对标本进行稀释，使稀释后的标本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。标本使用0.01mol/L 的PBS 稀释(PH=7.0-7.2)。3、若所检样本不包含在说明书所列样本之中，建议进行预实验验证其有效性，并注意留存样本。4、使用化学裂解液制备的组织匀浆或细胞提取液可能会由于某些化学物质的引入导致ELISA 实验结果偏差。5、若样本为细胞培养上清，因该类样本干扰因素较多，如：细胞状态、细胞数量、采样时间等，所以可能存在检测不出的情况。6、某些天然蛋白或重组蛋白，包括原核及真核重组蛋白，可能因为与本产品所使用的检测抗体及捕获抗体不匹配，而不被检测出。7、建议使用新鲜样本，保存时间过长可能会存在蛋白降解或变性导致实验结果偏差。[操作步骤]1、加样：分别设标准孔、待测样品孔、空白孔。设标准孔7 孔，依次加入100μL 不同浓度的标准品(见试剂准备2)。空白孔加100μL(见试剂准备第二步最后一管)，余孔加待测样品100μL，酶标板加上覆膜，37oC 温育2 小时。2、弃去液体，甩干，不用洗涤。3、每孔加检测溶液A 工作液100μL(临用前配制)，酶标板加上覆膜，37oC 温育1 小时。4、弃去孔内液体，每孔用350μL 的洗涤液洗涤，浸泡1-2 分钟，吸去(不可触及板壁)或甩掉酶标板内的液体，在实验台上铺垫几层吸水纸，酶标板朝下用力拍几次(也可轻拍将孔内液体拍干)，重复洗板3 次。最后一次洗涤后，要把孔内的洗涤液完全甩干。自动洗板机亦可。5、每孔加检测溶液B 工作液(临用前配制)100μL，加上覆膜，37oC 温育30 分钟。6、弃去孔内液体，甩干，洗板5 次，方法同步骤4。7、每孔加底物溶液90μL，酶标板加上覆膜，37oC 避光显色(反应时间控制在15-25 分钟，不要超过30 分钟。当标准孔的前3-4 孔有明显的梯度蓝色，后3-4 孔梯度不明显时，即可终止)。8、每孔加终止溶液50μL，终止反应，此时蓝色立转黄色。终止液的加入顺序应尽量与底物溶液的加入顺序相同。如出现颜色不匀一，请轻轻晃动酶标板以使溶液混合均匀。9、在确保酶标板底无水滴及孔内无气泡后，立即用酶标仪在450nm 波长测量各孔的光密度(O.D.值)。注意：1、试剂准备：准备一次实验所需要的酶标条，其它的可从微孔板上拆下，密封，按照说明书要求保存，以备下次使用。2、加样：实验操作中请使用一次性的吸头，避免交叉污染。加样时注意不要有气泡，将样品加于酶标板底部，尽量不触及孔壁，轻轻晃动混匀。加样或加试剂时，第一个孔与最后一个孔加样之间的时间间隔如果太大，将会导致不同的“预温育”时间，从而明显地影响到测量值的准确性及重复性。因此，一次加样时间(包括标准品及所有样品)最好控制在10 分钟内。推荐设置复孔进行实验。3、温育：为防止样品蒸发，实验时请将加上盖或覆膜的酶标板置于湿盒内，以避免液体蒸发，洗板后应尽快进行下步操作，任何时侯都应避免酶标板处于干燥状态，同时应严格遵守给定的温育时间和温度。4、洗涤：充分的洗涤非常重要，在每次洗涤过程中，都要将洗涤液完全甩干。洗涤过程中反应孔中残留的洗涤液应在滤纸上拍干，勿将滤纸直接放入反应孔中吸水，同时要消除板底残留的液体和手指印，避免影响最后的酶标仪读数。如果有自动洗板机，应在熟练使用后再用到正式实验过程中。5、反应时间的控制：加入底物后请定时观察反应孔的颜色变化(比如，每隔10 分钟观察一次)，如颜色较深，请提前加入终止液终止反应，避免反应过强从而影响酶标仪光密度读数。6、底物：底物请避光保存，在储存和温育时避免强光直接照射。7、如果实验室内湿度低于60%，推荐使用加湿器提高湿度水平。[实验原理]将Flt3L 抗体包被于96 孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的Flt3L 与连接于固相载体上的抗体结合，然后加入生物素化的Flt3L 抗体，将未结合的生物素化抗体洗净后，加入HRP 标记的亲和素，再次彻底洗涤后加入TMB 底物显色。TMB 在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Flt3L 呈正相关。用酶标仪在450nm 波长下测定吸光度(O.D.值)，计算样品浓度。[计算]各标准品及样本O.D.值扣除空白孔O.D.值后作图(七点图)，如设置复孔，则应取其平均值计算。以标准品的浓度为纵坐标(或对数坐标)，O.D.值为横坐标(或对数坐标），绘出标准曲线(最佳方程式应依回归方程计算的R2值来定，以R2 值越趋近于1 为好)。推荐使用专业制作曲线软件进行分析，如curve expert 1.30，根据样品O.D.值，由标准曲线查出相应的浓度，乘以稀释倍数；或用标准物的浓度与O.D.值计算出标准曲线的回归方程式，将样品的O.D.值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。[典型数据]为了便于计算，尽管浓度为自变量而O.D.值为因变量，我们绘图时仍采用标准品的O.D.值作为横坐标(X 轴)，标准品的浓度为纵坐标(Y 轴)。同时为了试验结果的直观性，图中提供的是原始数据而非对数值。推荐使用对数值建立标准曲线图。由于实验操作条件的不同（如操作者、移液技术、洗板技术和温度条件等），标准曲线的O.D.值会有所差异。所提供的标准曲线仅供参考，实验者需要根据自已的实验建立标准曲线。人FMS 样酪氨酸激酶3 配体(Flt3L)检测试剂盒标准曲线[检测范围]31.2pg/mL-2,000pg/mL[最低检测限]12.5pg/mL此值为20 个空白样品(即标准品稀释液)测定的平均值加二倍标准差所对应的浓度。[特异性]本试剂盒用于检测Flt3L，经检测与其它相似物质无明显交叉反应。由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。[回收率]分别于定值血清及血浆样本中加入一定量的Flt3L（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。样本回收率范围(%) 平均回收率(%)血清(n=5) 80-98 93EDTA 血浆(n=5) 82-95 90肝素血浆(n=5) 94-104 99[线性]在定值血清及血浆样本内加入适量的Flt3L，并倍比稀释成1:2，1:4，1:8，1:16 的待测样本，线性范围即为稀释后样本中Flt3L 含量的测定值与理论值的比率。样本1：2 1：4 1：8 1：16血清(n=5) 95-103% 91-101% 96-105% 82-94%EDTA 血浆(n=5) 84-102% 96-105% 83-97% 81-98%肝素血浆(n=5) 85-96% 79-91% 80-92% 97-105%[精密度]精密度用样品测定值的变异系数CV 表示。CV(%) = SD/mean×100批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD 值。批间差：选取3 个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8 次，分别计算不同浓度样本的平均值及SD 值。批内差： CV批间差： CV[稳定性]经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。[实验流程]1、实验前标准品、试剂及样本的准备；2、加样（标准品及样本）100μL，37oC孵育2小时；3、吸弃，加检测溶液A100μL，37oC孵育1小时；4、洗板3次；5、加检测溶液B100μL，37oC孵育30分钟；6、洗板5次；7、加TMB底物90μL，37oC孵育15－25分钟；8、加终止液50μL，立即450nm读数。[说明]1、由于现有条件及科学技术水平尚不能对所有供货商提供的所有原料进行全面的鉴定与分析，本产品可能存在一定的质量技术风险。2、最终的实验结果与试剂的有效性、实验者的相关操作以及当时的实验环境密切相关，请务必准备充足的标本备份。3、不同批次的同一产品可能会有少许差别，如：检测限、灵敏度以及显色时间等，请依据试剂盒内说明书进行实验操作，网站电子版说明书仅作参考。4、只有全部使用本试剂盒配套试剂才能保证检测效果，不能混用其他制造商的产品。只有严格遵守本试剂盒的实验说明才会得到最佳的检测结果。5、在储存及温育过程中避免将试剂暴露在强光中。所有试剂瓶盖须盖紧以防止蒸发和微生物的污染，因为蛋白水解酶的干扰将导致出现错误的结果。6、刚开启的酶联板孔中可能会有少许水样物质，此为正常现象，不会对实验结果造成任何影响。请勿提前将酶标板从包装袋里拿出。7、由于操作者不熟练、操作失误或读数仪程序选用错误等有可能会导致错误结果的产生。请使用者使用该产品前仔细阅读说明书，调试好酶标仪，请使用配备有450±10nm 滤光片的酶标仪，且该酶标仪测量范围在0.001-3.000 O.D.或以上。8、同一使用者在使用同一产品时，如不同时间订购，也可能会因不同批次的少量差异产生不同结果，因此建议使用者在每次使用前均进行预实验。9、试剂盒在出厂前均经过严格检测，但由于运输条件及各实验室条件差异，可能会造成实验结果与出厂结果不一致或不同批次试剂盒批间差大的情况，对于这种情况会酌情处理。10、本试剂盒未与其他厂家同类试剂盒或不同方法检测同一目的蛋白的产品做对比，平行检测可能会存在检测结果不一致的情况。11、本操作说明同样适用于48T 试剂盒，但48T 试剂盒所有试剂减半。[警告]本试剂盒中使用了稀硫酸作为终止液，其具有轻微腐蚀性，使用时应避免接触衣物或眼、手等皮肤暴露部位。[问题解答]问题可能原因解决方案标准曲线差标准品准备不正确进行正确的标准品梯度稀释吸取及洗涤不充分充分的吸取及洗涤移液不精确检查和校正移液器精密度低洗涤不充分按说明书要求充分洗涤和浸泡混匀不充分和吸取试剂不足充分混匀和吸取试剂重复利用吸头、容器和覆膜使用加样器要更换新的吸头、使用新的容器和覆膜加样不精确检查和校正移液器O.D 值低每孔加的试剂量不精确校正移液器，精确加入试剂温育时间不正确保证充足的温育时间温育温度不正确试剂要平衡至室温并保证准确的温育温度酶标记物或底物失效通过混合酶标记物和底物，颜色应迅速显现来检查没有加入终止液按照说明书实验操作步骤加入终止液超出读数时间读数在说明书推荐的读数时间内读数样本值不正确的样本储存方式正确储存样本，使用新鲜样本进行实验不正确的样本收集和处理方法采取正确的样本收集和处理方法待测物质在样本中含量低使用新鲜样本，重复实验

产品类型:ELISA Kit产品名称:Mouse Alpha-1B-glycoprotein(A1BG) ELISA kit英文名称:Mouse Alpha-1B-glycoprotein(A1BG) ELISA kit货号:XY-EL001001MO别名:A1B, ABG, DKFZp686F0970, GAB, HYST2477, alpha 1B-glycoprotein规格:96T中文价格:3600种属:Mouse待测物名称:alpha-1-B glycoprotein缩写:A1BG检测范围:0.156 μg/ml-10 μg/ml灵敏度:0.039 μg/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmUnopened kit Unopened kitUnopened kitUnopened kitUnopened kitUnopened kitUnopened kitUnopened kitUnopened kitStore at 2 Store at 2 Store at 2 Store at 2 Store at 2 Store at 2 Store at 2 Store at 2 - 8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date 8°C. Do not use past kit expiration date 8°C. Do not use past kit expiration date 8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date 8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date 8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date 8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date 8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date 8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration date8°C. Do not use past kit expiration dateOpened kit Opened kitOpened kitOpened kitOpened kitOpened kitOpened kitCoated assay Coated assay Coated assay Coated assay Coated assay Coated assay Coated assay Coated assay plateplate plateplateMay be sto May be stoMay be sto May be stoMay be sto May be stoMay be stored for up to 1 month at red for up to 1 month atred for up to 1 month at red for up to 1 month atred for up to 1 month atred for up to 1 month atred for up to 1 month atred for up to 1 month atred for up to 1 month at red for up to 1 month atred for up to 1 month atred for up to 1 month at red for up to 1 month atred for up to 1 month atred for up to 1 month atred for up to 1 month at 2 - 8° C. C. 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If 8° C. If 8° C. If 8° C. If 8° C. If 8° C. If don’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at on’t make recent use, better keep it store at -20 °C .BiotinBiotin Biotin Biotin-antibody antibody antibody antibody antibody antibody antibody antibodyHRP -avidinavidinavidin avidinBiotinBiotin Biotin Biotin-antibody antibody antibody antibody antibody antibody antibody antibody Diluent DiluentDiluentDiluentMay be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 May be stored for up to 1 month at 2 - 8°C.8°C.8°C.HRP -avidin avidin avidin avidin avidin Diluent DiluentDiluentDiluentSample Sample Sample Sample Sample Diluent DiluentDiluentDiluentWash BufferWash BufferWash BufferWash BufferWash Buffer Wash BufferWash BufferWash BufferWash BufferWash BufferTMB SubstrateStop SolutionStop Solution Stop SolutionStop Solution Stop SolutionStop SolutionStop Solution Stop SolutionStop Solution*Provided this is within the expiration date of the kit.

产品类型:ELISA Kit产品名称:小鼠α1抗胰糜蛋白酶(AACT)ELISA Kit英文名称:Mouse Alpha1 Antichymotrypsin,AACT ELISA Kit货号:XY-E13727m规格:96T中文价格:3600种属:Mouse待测物名称:Alpha1 Antichymotrypsin,AACT缩写:AACT检测范围:15.6 ng/ml-1000 ng/ml灵敏度:3.9 ng/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmMATERIALS PROVIDEDReagents ReagentsReagentsReagentsReagentsReagentsQuantity QuantityQuantityQuantity QuantityAssay plate (Assay plate (Assay plate (Assay plate (Assay plate (Assay plate ( Assay plate ( Assay plate (Assay plate (Assay plate ( 12 x 8 coated Micro8 coated Micro 8 coated Micro8 coated Micro8 coated Micro8 coated Micro we ll s)1(96 wells)wells)wells) wells)wells)Standard (Freeze dried)2Biotin-antibody (100 x concentrate)1 x 120 μlHRP-avidin (100 x concentrate)1 x 120 μlBiotin-antibody Diluent1 x 10 mlHRP-avidin Diluent1 x 10 mlSample Diluent2 x 20 mlWash Buffer (25 x concentrate)1 x 20 mlTMB Substrate1 x 10 mlStop Solution1 x 10 mlAdhesive Strip (For 96 wells)4Instruction manual1

犬N端前脑钠素(NT-proBNP)酶联免疫分析试剂盒使用说明书本试剂盒仅供研究使用。检测范围： 96T100pg/ml - 3200pg/ml使用目的：本试剂盒用于测定犬血清、血浆及相关液体样本中N 端前脑钠素(NT-proBNP)含量。实验原理本试剂盒应用双抗体夹心法测定标本中犬N 端前脑钠素(NT-proBNP)水平。用纯化的犬N 端前脑钠素(NT-proBNP)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入N 端前脑钠素(NT-proBNP)，再与HRP 标记的N 端前脑钠素(NT-proBNP)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB 显色。TMB 在HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的N 端前脑钠素(NT-proBNP)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），通过标准曲线计算样品中犬N 端前脑钠素(NT-proBNP)浓度。试剂盒组成1 30 倍浓缩洗涤液 20ml×1 瓶 7 终止液 6ml×1 瓶2 酶标试剂 6ml×1 瓶 8 标准品（6400 pg/ml） 0.5ml×1 瓶3 酶标包被板 12 孔×8 条 9 标准品稀释液 1.5ml×1 瓶4 样品稀释液 6ml×1 瓶 10 说明书 1 份5 显色剂A 液 6ml×1 瓶 11 封板膜 2 张6 显色剂B 液 6ml×1/瓶 12 密封袋 1 个标本要求1．标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融2．不能检测含NaN3 的样品，因NaN3 抑制辣根过氧化物酶的（HRP）活性。操作步骤1. 标准品的稀释：本试剂盒提供原倍标准品一支，用户可按照下列图表在小试管中进行稀释。3200 pg/ml 5 号标准品 150μl 的原倍标准品加入150μl 标准品稀释液1600 pg/ml 4 号标准品 150μl 的5 号标准品加入150μl 标准品稀释液800 pg/ml 3 号标准品 150μl 的4 号标准品加入150μl 标准品稀释液400 pg/ml 2 号标准品 150μl 的3 号标准品加入150μl 标准品稀释液200 pg/ml 1 号标准品 150μl 的2 号标准品加入150μl 标准品稀释液22. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、标准孔、待测样品孔。在酶标包被板上标准品准确加样50μl，待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品最终稀释度为5 倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。3. 温育：用封板膜封板后置37℃温育30 分钟。4. 配液：将30 倍浓缩洗涤液用蒸馏水30 倍稀释后备用。5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30 秒后弃去，如此重复5 次，拍干。6. 加酶：每孔加入酶标试剂50μl，空白孔除外。7. 温育：操作同3。8. 洗涤：操作同5。9. 显色：每孔先加入显色剂A50μl，再加入显色剂B50μl，轻轻震荡混匀，37℃避光显色15 分钟。10. 终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。11. 测定：以空白空调零，450nm 波长依序测量各孔的吸光度（OD 值）。 测定应在加终止液后15 分钟以内进行。操作程序总结：计算3以标准物的浓度为横坐标，OD 值为纵坐标，在坐标纸上绘出标准曲线，根据样品的OD 值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD 值计算出标准曲线的直线回归方程式，将样品的OD 值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。注意事项1．试剂盒从冷藏环境中取出应在室温平衡15-30 分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。2．浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。3．各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间最好控制在5 分钟内，如标本数量多，推荐使用排枪加样。4． 请每次测定的同时做标准曲线，最好做复孔。如标本中待测物质含量过高（样本OD 值大于标准品孔第一孔的OD 值），请先用样品稀释液稀释一定倍数（n 倍）后再测定，计算时请最后乘以总稀释倍数（×n×5）。5． 封板膜只限一次性使用，以避免交叉污染。6．底物请避光保存。7．严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.8．所有样品，洗涤液和各种废弃物都应按传染物处理。9．本试剂不同批号组分不得混用。10. 如与英文说明书有异，以英文说明书为准。保存条件及有效期1．试剂盒保存：；2-8℃。2．有效期：6 个月

4月15日-19日，第43届日内瓦国际发明展在日内瓦Palexpo展览馆举行。4月17日，组委会公布了各项大奖的得主，其中香港水中银(国际)生物科技有限公司的生物测毒技术从来自48个国家和地区的千余件新发明中脱颖而出，获本届日内瓦发明展大奖。48至72小时测逾千种毒该项技术利用转基因工程，可使斑马鱼鱼卵准确识别特定外界毒素侵入，若有急性有毒物质，斑马鱼便会畸形，验不到有毒便交给经基因改造的鲭鳉鱼胚胎测试是否有雌激素，有就会产生荧光的蛋白，在48至72小时内快速界定检测毒素种类，可测试超过1000种毒素。该套模型可以模拟人类药物新陈代谢的活动，从而预测样品对斑马鱼及人类的毒性。目前，传统化测试每次只能检测5到10种毒素，对未知样品的毒性检测只有20%的准确率，因此出现了很多“漏网之鱼”没有被检测。比起常规化学测试方法，该公司研发的“转基因鲭鳉鱼”及“斑马鱼”胚胎毒理测试技术的安全标准能够从30分提高到80分。目前其技术已获欧洲、中国内地、香港等地的政府相关部门、学术研究单位、跨国化妆品集团及食品集团等认可与采用。技术机理身体只有三、四厘米的鲭鳉鱼身形细小，孵化后只需三个月即达到生殖成熟期，鲭鳉鱼与栖息于淡水的日本鲭鳉鱼非常相近，加上研究人员对日本鲭鳉鱼的基因组及身体结构十分了解，因此选用鲭鳉鱼来研究毒素对环境的影响。水中银首席技术官陈雪平解释，转基因鲭鳉鱼主要检验慢性污染，研究人员将在鲭鳉鱼基因和部分水母基因的混合DNA植入鲭鳉鱼，变成会游泳的试管，在受雌激素物质污染的水域中，约五小时，鱼体内会像绿色灯泡一样发光。受感染的鲭鳉鱼胚胎会畸形，头部和尾部会烂掉，身体会出现肿胀，出现肿瘤甚至死亡。未来前景日内瓦国际发明展创办于1973年，是世界上举办历史最长、规模最大的发明展之一。组委会表示，这种技术可被应用在检测食品、药品、化妆品、塑料制品或饮用水等其他与人体接触的领域，能有效检测产品的整体毒性、致毒目标器官与致毒机制。近年来，食品安全问题屡见不鲜，随着三聚氰胺、地沟油、塑化剂等一系列食品安全问题发生，也倒逼了相关检测技术的升级换代。该技术革新了现有的化学检测，将有效提高全球食用品安全检测基准。

近日，来自瑞士的科学家们表示，在肺癌患者机体血液中循环的癌症DNA可以为医生们提供重要的突变信息，来帮助医生在患者肿瘤组织不易靶向作用的情况下对癌症疗法进行优化；相关研究为有效利用癌症疗法来靶向作用特殊癌症的突变提供了新的思路。检测肿瘤自身的突变并不总是有可能，然而很多研究都表明，循环在患者机体血液中的肿瘤DNA或许可以提供一些重要的指示信息。这项研究中研究者旨在对比标准方法检测肿瘤和利用血液检测EGFR突变之间的差异。Reck说道，即使肺部肿瘤不能通过支气管窥镜检查或CT引导活检法来发现，是否存在一种可用的方法可以帮助检测EGFR的突变并且可以提供开发超级疗法的思路呢？而且是否血液检测方法可以和金标准法得到同样的结果呢？本文研究中，研究者对1162份匹配的组织和血液样本进行了检测，结果显示血液检测手段和标准的组织检测方法得到的结果有89%的一致性，而且血液检测可以鉴别出一半的EGFR突变患者，而组织检测的敏感性仅为46%。Rafael Rosell表示，对于癌症患者而言，无细胞的血液DNA检测是一种有效的工具来帮助检测肿瘤组织的遗传改变，相关的研究结果也可以帮助科学家们证实，在几乎一半的肺癌患者血液或血浆中中可以直接检测到其携带的EGFR突变。本文研究或可帮助改进当前的技术，来进行肺癌患者的EGFR突变敏感性检测；该研究也为后期进行更为深入的研究来扩展检测突变的常规应用，比如EGFR突变可以作为治疗癌症患者的一部分。

近日，发表于国际杂志JAMA Psychiatry上的研究论文中，来自华盛顿大学医学院的研究者通过研究表示，抑郁症和2型糖尿病或许都和痴呆症的风险增加直接相关，而该风险在那些被诊断既是抑郁症又是糖尿病的患者中尤其高。糖尿病和严重抑郁在西方国家非常常见，大约有20%的糖尿病患者会患上抑郁症。研究者Dimitry Davydow表示，我们对240万丹麦居民进行了相关研究，检测了抑郁症患者、2型糖尿病患者以及同时患两种病的患者相比未患病的患者患痴呆症的风险，参与者年龄都在50岁及以上，其在2007年至2013年均无痴呆症病症表现。总的来讲，研究小组中有19.4%的个体为抑郁症（477，133名个体），有9.1%的个体为2型糖尿病（223，174名个体），有3.9%的个体均患上了两种病（95，691名个体），起初诊断为2型糖尿病的个体年龄为63.1岁，最初被诊断为抑郁症的年龄则为58.5岁。研究者表示，在研究期间有2.4%的个体患上了痴呆症（59，663名个体），平均年龄为81岁，在患痴呆症的个体中，15729名个体（26.4%）仅为抑郁症患者，6466名个体（10.8%）仅为2型糖尿病患者，同时4022名个体（6.7%）均患上了两种疾病。研究结果表明，仅患2型糖尿病和痴呆症风险增加20%直接相关，而仅患抑郁症则和痴呆症风险增加83%相关，共患两种病则和痴呆症风险增加117%相关，而且痴呆症的患病风险似乎在65岁以上的参与者中尤其高。从慢性病增加社会负担的角度考虑，未来研究中研究者需要阐明和抑郁症，2型糖尿病发生相关的病理学机制，以及揭示引发患者不良后果的原因，比如痴呆症等，从而为开发新型干预措施来抑制相关疾病并发症的发生提供新的线索和思路。

在癌症治疗中，肿瘤特异性抗体至比较常用的一类药物治疗手段。抗体的效用依赖于其与体内Fc gamma 受体的相互作用。目前抗体的治疗已经被用于多种恶性肿瘤中，然而，在相当多的患者体内出现了抗体药物的耐药性症状。为了解释这一症状的内在原因以及找到合适的方法避免耐药性的产生，抗体作用的分子机制需要得到进一步的探究。Rituximab（利妥昔单抗）是目前得到批准的，I型人源CD20单抗，被用于治疗B淋巴瘤以及滤泡细胞瘤等癌症疾病。然而，临床结果显示Rituximab在某些癌症中具有很好地疗效，而在其它类型的癌症中则没有效果；另外，即使在有效的癌症类型中，有的患者也表现出明显的耐药特征。之前的研究已经发现，Rituximab通过被体内的一种叫做FcgRIIB的抑制性受体识别并结合，从而导致该抗体被内吞，从而失去杀伤活性。在最近一期《cancer cell》杂志上，来自英国南安普顿大学的Mark S. Cragg开发出了一类针对FcgRIIB的抗体，能够有效阻断FcgRIIB对Rituximab的内吞作用，从而促进Rituximab的肿瘤杀伤能力。首先，作者利用FcgRII广谱性抗体AT-10与Rituximab联合用药，在小鼠肿瘤模型上进行实验，结果显示：AT-10能够大大提高Rituximab的肿瘤杀伤效果。由于AT-10既针对FcgRIIB也针对FcgRIIA，因此它不能用于临床治疗。作者通过噬菌体表面展示系统筛选了一系列人源的抗体，找到那些能够与FcgRIIB结合但同时不与FcgRIIA结合的抗体。最终，他们找到了一系列抗体（以5C04与6G11为代表）。Elisa检测显示，这些抗体能够特异性地与FcgRIIB结合而不与FcgRIIA结合。通过细胞水平的分析，作者证明了这些抗体只特异性识别B细胞，而非其它淋巴细胞类型。由于Rituximab与FcgRIIB通过Fc端的结合导致下游ITIM的磷酸化，从而导致该受体的激活。作者通过突变的方式将筛选到的抗体Fc端变异，从而使其不能够通过Fc端的方式与FcgRIIB结合（即只保留其特异性识别的能力），研究结果显示：在这些抗体中，一部分能够导致ITIM的磷酸化提高（比如5C04），另一部分则没有该效应。作者因此选择了能够特异性结合FcgRIIB但不激活其下游信号的抗体作为阻断型抗体候选物进行下一步研究。他们将这些抗体与Rituximab共同刺激B细胞，结果显示：这些抗体中有一部分能够有效抑制Rituximab对下游ITIM的磷酸化效应，另一部分则没有该功能。之后，作者也证明了这些筛选到的抗体能够有效阻止Rituximab的内吞效应之后，作者通过体外的实验证明：6G11能够增强Rituximab对细胞的杀伤效应，而这一效应并不依赖于其Fc端的结构。同时，作者还证明了6G11能够抑制Rituximab受FcgRIIB识别而被内吞的影响。这一结果揭示了6G11的潜在抗肿瘤的功能。最后，作者通过一些列体内实验证明了6G11对Rituximab的增强作用，同时也证明了它不会引起"细胞因子风暴"等免疫异常反应。

近日，刊登在国际杂志Cell上的一篇研究论文中，来自德克萨斯大学MD安德森癌症中心的研究人员通过研究表示，利用免于免疫系统攻击癌症的药物同基因组靶向疗法相结合或许可以更好地帮助治疗癌症患者。靶向疗法：频繁但却反应短暂在过去30年里研究者已经阐明了许多参与癌症发生的分子机制，而同时研究者也鉴别出了许多可以诱发癌症的遗传突变，而针对这些致癌的遗传缺陷的靶向药物被证明在很多患者的初期治疗中是有作用的，比如靶向作用BRAF基因突变的药物在几乎一半的黑色素瘤患者中都可以抑制肿瘤的发生；然而耐药性随之而来，因为肿瘤拥有多种基因组缺失，其就可以帮助肿瘤细胞在药物疗法后产生一种耐药性，BRAF抑制剂在临床试验中可以将患者的生存中值延长至7个月。检查点封锁：较少但结果却较为强劲研究者Allison提出了“免疫检查点封锁”一说，这是一种治疗免疫系统的方法，通过阻断T细胞上的特殊分子来间接地保护肿瘤细胞免于免疫反应；而一种名为易普利姆玛（Ipilimumab）的药物在抵御恶性黑色素瘤上相比其它靶向药物表现出了较低的免疫反应率，但长期跟踪调查结果显示，22%的治疗患者至少存活了4年，更重要的是那些幸存3年的患者甚至生活超过了10年。免疫力是长期反应的关键众所周知，免疫系统可以识别癌细胞的特性，并且发动T细胞来攻击肿瘤细胞的抗原，而检查点封锁正好可以移除这种攻击作用；研究者需要理解为何某些癌症患者对免疫疗法没有反应，但是对于其他患者而言反应却是非常明显的。什么样的组合方式可以更有效地抗癌？研究者Sharma指出，多种基因组靶向疗法的结合被证实可以有效抑制癌症，然而很多数据又显示，肿瘤基因组的多样性或许会打败许多组合疗法，而且随着不同药物对患者的施用也会增加更大的副作用。靶向疗法或许可以扮演一种有效的癌症疫苗，其可以杀灭肿瘤细胞并且释放T细胞最喜爱的靶向抗原来对癌细胞实施打击。研究者的I期试验是将免疫检查点封锁药物同两种靶向疗法相结合，而后期研究者并没有足够的时间来确定个体的生存影响及机体反应的持久性。而在两项临床试验中，研究者将易普利姆玛同两种不同的BRAF抑制剂相结合来阐明联合疗法在治疗黑色素瘤中的作用，在其中一种疗法中肝脏的毒性导致试验终止，而另一种组合疗法随着试验的进行也表现出了较好的耐受性。研究者表示，这就需要强调药物、剂量以及给药方案的差异，其需要被评估才可以进行不同联合疗法的开发，当前检查点阻断药物主要可以阻断两种“检查点”机制，而研究中发现的其它机制可以同刺激性分子一样来刺激机体产生免疫反应；这就为免疫疗法提供了新的靶点。面对多种可能的药物组合更有效的临床前研究或许可以帮助研究者做出正确合理的选择。给予免疫疗法研究支持由于大量的资金都投入到了癌症定向的基因组靶向疗法中去，因此研究者不得不暂时搁置免疫疗法的开发；研究者Sharma及其同事倡议，应当投入大量的资源用于进行免疫检查点的疗法研究以及开发具有治愈潜能的靶向免疫组合性疗法。最后研究者总结道，当前阶段我们可以毫不牵强地说，增加对联合疗法的资助或许是未来开发有效治疗癌症的最佳选择，而研究者也将为了这一目的不断深入研究。

许多癌症患者遭受衰弱综合征，又称为恶病质，整个身体肌肉和脂肪组织破坏。发表在最近的Development Cell上两项使用果蝇为模型研究表示一个肿瘤分泌的蛋白质Impl2，能够抑制全身胰岛素信号，并且可能是驱动恶病质的罪魁祸首。肌肉消瘦和能量存储的损失发生在与癌症相关的恶病质，但也可以由严重饥饿，感染，或响应于创伤触发。之前已经有研究通过移植肿瘤建立这种恶病质果蝇模型，不过很少有人了解，局部肿瘤如何引起广泛组织破坏的分子机制。要了解这个过程中，哈佛大学的Norbert Perrimon和他的合作者激活Yorkie（yki)-一种与人类YAP1同源的果蝇基因，导致果蝇肠细胞繁殖过剩。6至12天后，这个局部的细胞生长造成脂肪和肌肉组织退化。在肌肉中这种退化是伴随着有关碳水化合物和蛋白质代谢基因的表达减少，和血液循环中显着增高的糖水平。对果蝇的转录组进一步分析后发现，在繁殖过剩的肠细胞中Impl2表达约为70倍以上。这种蛋白在肌肉，脂肪，卵巢和其他组织中的表达对比于对照动物的组织并没有什么不同。干扰Impl2表达后，防止恶病质带来的组织萎缩和消耗，表明该组织特异性分泌的因子可调节介导整个能量存储系统。这项研究使用了一个致癌基因同源物激活Impl2，但可能其他致癌基因可以发挥类似的作用。果蝇的Impl2功能很像哺乳动物的胰岛素样生长因子（IGF）结合蛋白，能够阻断胰岛素信号。一些研究表明，这些蛋白质在癌症中被上调，但它们在癌症恶病质的作用并不被充分了解。研究人员表示他们将会可以筛选果蝇基因组，看看到底那些蛋白参与了恶病质过程。加州大学伯克利分校的 Alejandra Figueroa-Clarevega和David Bilder，在果蝇腹部移植肿瘤诱导恶病质。他们发现恶性肿瘤会诱导的脂肪，肌肉和性腺组织发生大规模破坏。而良性的肿瘤可以长到比恶性肿瘤更大，但即使它们非常大，却不会引起这种恶病质。这表明并不是因为肿瘤越大越需要消耗营养物质，取而代之的原因是一些恶性肿瘤产生的一些物质造成了恶病质。通过转录组分析搜索这个分泌因子，研究人员同样发现了有嫌疑的Impl2。肿瘤分泌Impl2降低其他组织的胰岛素信号传导途径。在随后的实验中研究人员发现，即使不存在肿瘤，简单地在肠细胞过表达Impl2就能够触发远侧组织的变性。而在肿瘤组织中去除Impl2表达，既能可以防止恶病质症状。在两项研究中，有肿瘤的果蝇并不比健康的果蝇进食少。研究人员说道：“关于恶病质的关键是，你不能只通过病人进食或者打营养素来对抗恶病质。”两篇研究同时锁定到Impl2，表示Impl2的确对恶病质有作用。无论Impl2是否在人类癌症恶病质中也扮演同样重要的角色，这些发现都有重大意义。我们目前无法治疗癌症恶病质的主要原因是缺乏对其发展的机制了解。Impl2鉴定作为潜在的作用因子，可以提高我们对这个疾病的认识。xy-E10133 人肿瘤坏死因子β(TNF-β)ELISA试剂盒 96t一盒xy-E10134 人肿瘤坏死因子α(TNF-α)ELISA试剂盒 96t一盒xy-E10135 人肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)ELISA试剂盒 96t一盒xy-E10136 人肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒 96t一盒xy-E10137 人转化生长因子β1(TGF-β1)ELISA试剂盒 96t一盒xy-E10138 人转化生长因子α(TGF-α)ELISA试剂盒 96t一盒xy-E10139 人基质细胞衍生因子1β(SDF-1β/CXCL12)ELISA试剂盒 96t一盒xy-E10140 人干细胞因子受体(SCFR)ELISA试剂盒 96t一盒xy-E10141 人干细胞因子/肥大细胞生长因子(SCF/MGF)ELISA试剂盒 96t一盒xy-E10142 人可溶性CD40配体(sCD40L)ELISA试剂盒 96t一盒xy-E10143 人可溶性CD30配体(sCD30L)ELISA试剂盒 96t一盒xy-E10144 人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒 96t一盒xy-E10145 人P选择素(P-Selectin/CD62P/GMP140)ELISA试剂盒 96t一盒xy-E10146 人血血小板衍生生长因子AB(PDGF-AB)ELISA试剂盒 96t一盒xy-E10147 人血血小板衍生生长因子可溶性受体α(PDGFsR-α)ELISA试剂盒 96t一盒xy-E10148 人神经营养因子4(NT-4)ELISA试剂盒 96t一盒xy-E10149 人神经营养因子3(NT-3)ELISA试剂盒 96t一盒xy-E10150 人的神经生长因子(NGF)ELISA试剂盒 96t一盒xy-E10151 人巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA试剂盒 96t一盒xy-E10152 人巨噬细胞炎性蛋白3α(MIP-3α/CCL20)ELISA试剂盒 96t一盒xy-E10153 人巨噬细胞炎性蛋白1β(MIP-1β/CCL4)ELISA试剂盒 96t一盒xy-E10154 人巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒 96t一盒xy-E10155 人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒 96t一盒xy-E10156 人巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒 96t一盒xy-E10157 人单核细胞趋化蛋白3(MCP-3/CCL7)ELISA试剂盒 96t一盒xy-E10158 人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒 96t一盒xy-E10159 人单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)ELISA试剂盒 96t一盒xy-E10160 人L选择素(L-Selectin/CD62L)ELISA试剂盒 96t一盒xy-E10161 人白介素9(IL-9)ELISA试剂盒 96t一盒xy-E10162 人白介素8(IL-8/CXCL8)ELISA试剂盒 96t一盒xy-E10163 人白介素6(IL-6)ELISA试剂盒 96t一盒xy-E10164 人白介素-5(IL-5)ELISA试剂盒 96t一盒

近日，国际学术期刊nature communication在线发表了美国纽约大学医学院的一项最新发现，他们发现在干细胞转录因子Sox2依赖性的癌症干细胞中，Sox2能够通过拮抗Hippo途径，解除Hippo信号途径对癌基因YAP的抑制作用，这对于维持癌症干细胞的多能性具有重要作用。因此，阻断Sox2对YAP的激活，抑制YAP活性或许是治疗Sox2依赖性肿瘤的一个重要手段。Hippo信号通路在肿瘤抑制方面具有重要作用，通过该信号通路可以限制具有促生长功能的转录共激活因子YAP。研究人员在之前的研究中发现干细胞转录因子SOX2可以维持骨肉瘤中癌症干细胞的多能性。在该研究中，研究人员发现SOX2能够直接抑制Hippo通路的两个激活因子，Nf2和WWC1，拮抗Hippo信号通路对YAP的抑制作用，导致YAP功能过度激活。 在肿瘤细胞群体中，癌症干细胞的Nf2，WWC1两个因子受到抑制并且YAP高水平表达，这是Sox2依赖性肿瘤中癌症干细胞的标记，而分化程度更高的肿瘤细胞群体具有更高水平的Nf2和WWC1，但YAP表达受到抑制。敲除YAP能够显著减少癌症干细胞数量，并降低骨肉瘤的发生。因此，研究人员得出结论，在骨肉瘤中，SOX2能够通过干扰具有肿瘤抑制功能的Hippo信号途径维持癌症干细胞。除了在骨肉瘤中具有上述机制，研究人员同时发现SOX2-Hippo之间的关系在其他SOX2依赖性的肿瘤中，如胶质母细胞瘤，也同样存在。因此，抑制YAP转录活性或可成为治疗SOX2依赖性肿瘤的一条有效治疗策略，具有潜在应用价值。 xyB780Hu 人类粘蛋白2(ORM2)ELISA试剂盒xyB781Hu 人磷脂爬行酶1(PLSCR1)ELISA试剂盒xyB785Hu 人脑啡肽酶(NEP)ELISA试剂盒xyB792Hu 人S100钙结合蛋白A8(S100A8)ELISA试剂盒xyB793Hu 人世间S100钙结合蛋白A9(S100A9)ELISA试剂盒xyB795Hu 人血清淀粉样蛋白A2(SAA2)ELISA试剂盒xyB796Hu 人信号传导转录激活因子2(STAT2)ELISA试剂盒xyB801Hu 人激肽释放酶原(PK)ELISA试剂盒xyB805Hu 人高分子量激肽原(HMWK)ELISA试剂盒xyB808Hu 人粘蛋白7(MUC7)ELISA试剂盒xyA680Hu 人棕榈酰化膜蛋白2(MPP2)ELISA试剂盒xyA681Hu 人乙醇脱氢酶1(ADH1)ELISA试剂盒xyB810Hu 人维生素D结合蛋白(DBP)ELISA试剂盒xyB817Hu 人多功能蛋白聚糖(VS)ELISA试剂盒xyB818Hu 人血管内皮生长因子受体1(VEGFR1)ELISA试剂盒xyB819Hu 人再生胰岛衍生蛋白4(REG4)ELISA试剂盒xyB822Hu 人角蛋白17(KRT17)ELISA试剂盒xyB827Hu 人轴突生长诱向因子1(Ntn1)ELISA试剂盒xyB835Hu 人轴突生长诱向因子4(Ntn4)ELISA试剂盒xyB967Hu 人载脂蛋白A4(APOA4)ELISA试剂盒xyB829Hu 人解整合素金属蛋白酶9(ADAM9)ELISA试剂盒xyB830Hu 人组织转谷氨酰胺酶(TGM2)ELISA试剂盒xyB831Hu 人音猬因子(SHH)ELISA试剂盒xyA111Hu 人白介素12(IL12)ELISA试剂盒xyB833Hu 人补体因子D(CFD)ELISA试剂盒xyB834Hu 人凝血因子Ⅺ(F11)ELISA试剂盒xyB839Hu 人纽蛋白(VCL)ELISA试剂盒xyB848Hu 人核因子κB抑制因子α(IκBα)ELISA试剂盒xyB850Hu 人β-1,4-半乳糖转移酶1(β4GALT1)ELISA试剂盒xyA143Hu 人血管内皮生长因子A(VEGFA)ELISA试剂盒xyB852Hu 人含Rho关联卷曲螺旋蛋白激酶2(Rock2)ELISA试剂盒xyB859Hu 人凝集素样氧化低密度脂蛋白受体1(LOX1)ELISA试剂盒xyB860Hu 人胎球蛋白B(FETUB)ELISA试剂盒xyB824Hu 人核因子κB(NFκB)ELISA试剂盒xyB856Hu 人Ⅰ类主要组织相容性复合体G(MHCG)ELISA试剂盒xyB866Hu 人神经调节素1(NRG1)ELISA试剂盒

使用小鼠肌钙蛋白T(TNT)ELISA试剂盒检测过程中值得关注的问题浓缩：由于净化过程中引入的溶剂，可能会降低待测组分的浓度或者不适宜直接分析，需要去除全部有机溶剂。即试剂盒前处理步骤中把样本在60℃氮气下吹干，再用复溶液溶解干燥残留物。浓缩方式：氮气吹干除杂、压缩空气吹干除杂。注意：1在吹干样本之前，用甲醇清洗针头，防止杂质干扰。2在吹样本时，针头应在液面上空，避免与样本接触，防止产生交叉污染。3样本吹干后应立即取下，避免吹的时间过长，影响最终检测结果。4不同的药物，吹干后样本的保质期不同，提倡待样本回到室温后立即复溶。5 净化：经过提取的待测组分中通常含有一些会干扰试剂盒中抗原抗体反应的杂质或者是含有与待测物结构相似的杂质。将待测组分与杂质分离的过程，我们称为小鼠肌钙蛋白T(TNT)ELISA试剂盒中样本的净化。在我们现有的试剂盒前处理方法中涉及到的最常见的净化是：液液分配法。比如：用复溶液溶解干燥残留物之前先加入适量的正己烷，在加入正己烷和复溶液涡动后如果出现乳化现象，可以60℃水浴加热，至乳化现象消失或减弱后，加大离心转速进行离心。实例：A、氟喹诺酮类药物试剂盒现有的水产样本前处理方法中，用二氯甲烷作为提取剂。注意： (a)二氯甲烷的密度比水大，离心完后，二氯甲烷在下层，须先用加样器吸尽上层废液后，再按要求取适量的下层吹干。 (b)在用加样器吸取下层的有机相时，正常操作往往取量不准确，此时最好用带有刻度，且刻度较准确的玻璃管来定量。 (c)上诉情况对有经验的试验员还可以通过灵活控制加样器来控制取样的准确性。 (d)在振荡过程中要注意安全。二氯甲烷在振荡的过程中，如果离心管密封性不是很好，往往容易爆开；在进行振荡时，不要让离心管口对准自己或者其他人，避免溅到自己或者他人身上。 (e)小鼠肌钙蛋白T(TNT)ELISA试剂盒在使用前充分回温很必要，如回温不充分该项目曲线受影响较明显。B、硝基呋喃代谢物检测过程中常见问题 硝基呋喃代谢物有四种：3-氨基-2-唑烷基酮(AOZ)、5-甲基吗啉-3-氨基-2-唑烷基酮(AMOZ)、氨基脲(SEM)和1-氨基-2-内酰脲(AHD)在检测过程中也需要同其它项目一样进行添加回收测评，其中需要注意以下几个问题。  代谢物在组织中是与蛋白呈结合状态，采用普通的有机溶剂或缓冲液，是无法提取该代谢物的。需要用盐酸水解后才能使呋喃类代谢物游离，所以在加入去离子水、盐酸和2-硝基苯甲醛(2-NBA)混合均匀后，其pH应在1-3之间，否则不利用水解代谢物。在衍生化过程中，温度和时间决定其衍生化产率，衍生化后在加入氢氧化钠和磷酸氢二钾时，其pH应在中性左右。由于部分样本的缓冲能力不同，所以需对其pH进行测定，因为在酸性或碱性环境中，不利于呋喃类代谢物的提取回收，同时也容易出现假阳性。小鼠肌钙蛋白T(TNT)ELISA试剂盒结果判断定性测定   定性测定的结果判断是对受检标本中是否含有待测抗原或抗体作出"有"或"无"的简单回答，分别用"阳性"、"阴性"表示。"阳性"表示该标本在该测定系统中有反应。"阴性"则为无反应。用定性判断法也可得到半定量结果，即用滴度来表示反应的强度，其实质仍是一个定性试验。在这种半定量测定中，将标本作一系列稀释后进行试验，呈阳性反应的最高稀释度即为滴度。根据滴度的高低，可以判断标本反应性的强弱，这比观察不稀释标本呈色的深浅判断为强阳性、弱阳性更具定量意义。小鼠肌钙蛋白T(TNT)ELISA试剂盒其他相关产品展示：xy-E10103 【中文名称】：人白介素27(IL-27)ELISA试剂盒 【英文名称】：Human interleukin- 27 (IL-27) ELISA kit 规格：96T/48T xy-E10104 【中文名称】：人白介素23(IL-23)ELISA试剂盒 【英文名称】：Human interleukin- 23 (IL-23) ELISA kit 规格：96T/48T xy-E10105 【中文名称】：人巨噬细胞移动抑制因子(MIF)ELISA试剂盒 【英文名称】：Human macrophage migration inhibitory factor (MIF) ELISA kit 规格：96T/48T xy-E10106 【中文名称】：人组织因子途径抑制物(TFPI)ELISA试剂盒 【英文名称】：Human tissue factor pathway inhibitor (TFPI) ELISA kit 规格：96T/48T xy-E10107 【中文名称】：人干扰素诱导蛋白10(IP-10/CXCL10)ELISA试剂盒 【英文名称】：Human interferon -inducible protein 10 (IP-10/CXCL10) ELISA kit 规格：96T/48T xy-E10108 【中文名称】：人白介素1(IL-1)ELISA试剂盒 【英文名称】：Human interleukin- 1 (IL-1) ELISA Kit 规格：96T/48T xy-E10109 【中文名称】：人白介素17(IL-17)ELISA试剂盒 【英文名称】：Human interleukin- 17 (IL-17) ELISA kit 规格：96T/48T xy-E10110 【中文名称】：人白介素1β (IL-1β)ELISA试剂盒 【英文名称】：Human interleukin- 1β (IL-1β) ELISA kit 规格：96T/48T xy-E10111 【中文名称】：人表皮生长因子(EGF)ELISA试剂盒 【英文名称】：Human epidermal growth factor (EGF) ELISA kit 规格：96T/48T xy-E10112 【中文名称】：人碱性成纤维细胞生长因子(bFGF)ELISA试剂盒 【英文名称】：Human basic fibroblast growth factor (bFGF) ELISA kit 规格：96T/48T xy-E10113 【中文名称】：人巨噬细胞炎性蛋白5(MIP-5)ELISA试剂盒 【英文名称】：Human macrophage inflammatory protein 5 (MIP-5) ELISA kit 规格：96T/48T xy-E10114 【中文名称】：人可溶性E选择素(sE-selectin)ELISA试剂盒 【英文名称】：Human soluble E -selectin (sE-selectin) ELISA kit 规格：96T/48T xy-E10115 【中文名称】：人可溶性细胞间粘附分子1(sICAM-1)ELISA试剂盒 【英文名称】：Between Human soluble intercellular adhesion molecule- 1 (sICAM-1) ELISA Kit 规格：96T/48T xy-E10116 【中文名称】：人细胞间粘附分子2(ICAM-2/CD102)ELISA试剂盒 【英文名称】：Between Human cell adhesion molecule 2 (ICAM-2/CD102) ELISA kit 规格：96T/48T xy-E10117 【中文名称】：人细胞间粘附分子3(ICAM-3/CD50)ELISA试剂盒 【英文名称】：Between Human cell adhesion molecule 3 (ICAM-3/CD50) ELISA kit 规格：96T/48T xy-E10118 【中文名称】：人结缔组织生长因子(CTGF)ELISA试剂盒 【英文名称】：Human connective tissue growth factor (CTGF) ELISA Kit 规格：96T/48T 

活性氧的代谢调控是多细胞生物维持机体平衡与正常发育非常重要的方面，此外，活性氧也被认为参与了多种免疫反应，包括T细胞的激活与增殖等。而活性氧的过度积累将会对细胞造成强烈的毒性刺激，导致细胞死亡。在产生方面，细胞内部活性氧的来源主要是NADPH氧化酶家族与线粒体呼吸链复合体；而在消化方面，主要由一类过氧化物酶来完成，Gpx4便是其中的一员。此前的研究显示，Gpx4能够抑制磷脂的过氧化以及这一效应造成的细胞凋亡。 虽然Gpx4对于活性氧的清除具有重要的作用，但他对于免疫系统又有怎样的影响还不清楚。最近来自瑞士苏黎世大学生物系的Manfred Kopf研究组在《JEM》杂志上发表了他们对于Gpx4在小鼠免疫反应中的作用机制研究。 首先，作者构建了T细胞特异性Gpx4敲除小鼠，并通过DNA，RNA，以及蛋白质的水平进行了验证。之后，他们比较了野生型小鼠与突变体小鼠个淋巴器官中T细胞的分布与比例情况。结果显示：对于CD4+T细胞来说，野生型与突变体小鼠中各淋巴器官（脾脏，外周淋巴结，肠系膜淋巴结）细胞的比例并没有明显区别；而对于CD8+细胞而言，突变体小鼠淋巴器官中细胞的比例发生了明显的下降。同时，作者通过流式方法检测了调节性T细胞以及激活后的T细胞的变化情况。发现不论Treg，CD4+，CD8+ ，它们在突变体与野生型小鼠中的比例并没有明显变化。以上事实表明，Gpx4的缺失明显抑制了CD8+的稳态增殖。 为了进一步说明Gpx4的缺失是否影响了T细胞的增殖能力。作者利用骨髓移植的方法将野生型小鼠与突变小鼠的骨髓细胞1:1混合打入放射后的受体（野生型）小鼠体内。一段时间后对胸腺细胞以及脾脏细胞进行分析。结果显示：胸腺细胞中的两种类型的细胞比例没有显著差异，而在脾脏中突变体小鼠的CD4+以及CD8+细胞的增殖情况相比野生型均受到了抑制。医生实验结果说明T细胞在外周的稳态增殖受到Gpx4的影响，而Gpx4并不影响胸腺细胞的稳态调节。 之后，作者们分别向野生型与突变体小鼠注入LCMV进行刺激，检测结果显示：突变体小鼠体内诱导产生的CD4+CD8+T细胞均明显下降。利用抗原特异性MHC-II或MHC-I四聚体对相应的T细胞进行筛选，也发现特异性的CD4+以及CD8+T细胞的增殖均受到了抑制。 之后，作者通过体外检测，发现突变体的T细胞（CD4+以及CD8+）特别容易引发脂类的过氧化反应以及铁中毒死亡，而且死亡与否与激活状态没有关系。 最后，作者通过tine注射维他命E，结果显示注射后的突变体小鼠表型得到了恢复，其抗病毒免疫反应也得到了增强。xyB622Hu 【中文名称】：人表面活性物质关联蛋白B(SPB)ELISA试剂盒 【英文名称】：ELISA Kit for Surfactant Associated Protein B (SPB) 规格：96T/48T xyB623Hu 【中文名称】：人表面活性物质关联蛋白C(SPC)ELISA试剂盒 【英文名称】：ELISA Kit for Surfactant Associated Protein C (SPC) 规格：96T/48T xyA656Hu 【中文名称】：人二胺氧化酶(DAO)ELISA试剂盒 【英文名称】：ELISA Kit for Diamine Oxidase (DAO) 规格：96T/48T xyB572Hu 【中文名称】：人淋巴细胞功能关联抗原1α(LFA1α)ELISA试剂盒 【英文名称】：ELISA Kit for Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) 规格：96T/48T xyB705Hu 【中文名称】：人防御素α1(DEFα1)ELISA试剂盒 【英文名称】：ELISA Kit for Defensin Alpha 1, Neutrophil (DEFa1) 规格：96T/48T xyB706Hu 【中文名称】：人脂质运载蛋白1(LCN1)ELISA试剂盒 【英文名称】：ELISA Kit for Lipocalin 1 (LCN1) 规格：96T/48T xyA753Hu 【中文名称】：人Toll样受体4(TLR4)ELISA试剂盒 【英文名称】：ELISA Kit for Toll Like ReHZptor 4 (TLR4) 规格：96T/48T xyB955Hu 【中文名称】：人白介素17F(IL17F)ELISA试剂盒 【英文名称】：ELISA Kit for Interleukin 17F (IL17F) 规格：96T/48T xyC019Hu 【中文名称】：人血小板激活因子乙酰水解酶2(PAFAH2)ELISA试剂盒 【英文名称】：ELISA Kit for Platelet Activating Factor AHZtylhydrolase 2 (PAFAH2) 规格：96T/48T xyB818Mu 【中文名称】：人血管内皮生长因子受体1(VEGFR1)ELISA试剂盒 【英文名称】：ELISA Kit for Vascular Endothelial Growth Factor ReHZptor 1 (VEGFR1) 规格：96T/48T xyC026Hu 【中文名称】：人Salusinβ蛋白(Salusinβ)ELISA试剂盒 【英文名称】：ELISA Kit for Salusin Beta 规格：96T/48T xyC027Hu 【中文名称】：人O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)ELISA试剂盒 【英文名称】：ELISA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) 规格：96T/48T xyC028Hu 【中文名称】：人白介素28B(IL28B)ELISA试剂盒 【英文名称】：ELISA Kit for Interleukin 28B (IL28B) 规格：96T/48T xyC029Hu 【中文名称】：人白介素29(IL29)ELISA试剂盒 【英文名称】：ELISA Kit for Interleukin 29 (IL29) 规格：96T/48T xyC031Hu 【中文名称】：人白介素4受体(IL4R)ELISA试剂盒 【英文名称】：ELISA Kit for Interleukin 4 ReHZptor (IL4R) 规格：96T/48T xyB828Hu 【中文名称】：人载脂蛋白C4(APOC4)ELISA试剂盒 【英文名称】：ELISA Kit for Apolipoprotein C4 (APOC4) 规格：96T/48T 

近日，国际学术期刊cell reports在线发表了美国科学家的一项最新研究进展，他们发现在小肠干细胞和小肠稳态调节过程中，Notch和Wnt信号扮演了重要调控作用，阻断Notch信号会引起小肠干细胞分化失衡，并且在这一过程中伴随对Wnt信号途径的抑制解除。 器官的稳态平衡需要对成体干细胞进行严格调控，并通过多种信号整合实现干细胞向终末细胞的分化过程。在小鼠的小肠中，Notch和wnt信号对于小肠干细胞维持以及肠道负责消化液分泌的细胞和负责营养吸收的细胞之间的分化平衡都是非常必要的。 研究人员发现当Notch信号受到抑制，小肠干细胞倾向于向分泌细胞方向分化，而影响营养吸收细胞的分化过程。研究人员利用具有功能阻断特性的抗体特异性阻断Notch受体，结果发现阻断Notch信号会解除对Wnt信号途径的抑制作用，扰乱小肠干细胞的正常功能，导致促分泌功能相关基因的错误表达。同时，研究人员还发现抑制wnt信号通路能够恢复因Notch信号阻断所造成的表型。 这项研究阐述了一种维持干细胞活性以及分化平衡的负向调节机制，并且还证明了Wnt与Notch信号之间的相互作用对与维持成体干细胞的正常功能具有重要意义。xyD370Hu 人Kruppel样因子15(KLF15)检测试剂盒xyB563Hu 人杀伤细胞免疫球蛋白样受体2DS4(KIR2DS4)检测试剂盒xyC743Hu 人甲状旁腺素受体1(PTHR1)检测试剂盒xyB735Hu 人胱天蛋白酶4(CASP4)检测试剂盒xyB510Hu 人白介素5受体α(IL5Rα)检测试剂盒xyH832Hu 人汗酸酶αL(IDUα)检测试剂盒xyL575Hu 人颗粒酶H(GZMH)检测试剂盒xyL918Hu 人信号素3B(SEMA3B)检测试剂盒xyL924Hu 人信号素5A(SEMA5A)检测试剂盒xyM011Hu 人肽酶D(PEPD)检测试剂盒xyM070Hu 人过氧蛋白同源物(PXDN)检测试剂盒xyM277Hu 人睾素2(TIC2)检测试剂盒xyM410Hu 人双解丝蛋白1(TWF1)检测试剂盒xyM635Hu 人牙骨质蛋白1(CEMP1)检测试剂盒xyM802Hu 人Juxtanodin蛋白(JN)检测试剂盒xyN221Hu 人Dermokine蛋白(DMKN)检测试剂盒xyQ200Hu 人二氢二醇脱氢酶2(DDH2)检测试剂盒xyQ336Hu 人信号素4B(SEMA4B)检测试剂盒xyQ483Hu 人防御素β126(DEFβ126)检测试剂盒xyQ485Hu 人钙黏蛋白26(CDH26)检测试剂盒xyQ656Hu 人防御素β124(DEFβ124)检测试剂盒xyQ660Hu 人防御素β131(DEFβ131)检测试剂盒xyS085Hu 人血管紧张素1-7(Ang1-7)检测试剂盒xyS270Hu 人YY肽3(PYY3)检测试剂盒xyS952Hu 人NADH脱氢酶5(ND5)检测试剂盒xyT844Hu 人血管紧张素1-9(Ang1-9)检测试剂盒xyA179Hu 人干扰素α2(IFNα2)检测试剂盒xyA200Hu 人核糖核酸酶H(RNASEH)检测试剂盒xyA238Hu 人酪胺酸酶(TYR)检测试剂盒xyA340Hu 人蛛毒素受体3(LPHN3)检测试剂盒xyA425Hu 人突触素(SYP)检测试剂盒xyA444Hu 人桥粒芯糖蛋白3(DSG3)检测试剂盒

近来一种能够激活人体自身免疫系统对付肿瘤的新药大获成功，然而，只有部分癌症患者得以受益。科学家们近来发现编码肺部肿瘤的基因序列有可能能够帮助我们预测免疫药物能够让哪些人受益最多。 肿瘤细胞之所以能否逃脱人体免疫系统的监视，是通过激活了免疫T细胞表面一个叫做PD-1的受体。该受体被激活以后，T细胞再也不会进攻肿瘤细胞而是只作壁上观。我们所说的新药是一种抗体，能够阻断PD-1和配体PD-L1之间的作用，从而解放T细胞，进而消灭肿瘤。在临床实验中，相比于传统的治疗手段，PD-1等抑制剂延长肿瘤病人的生存期多达数年之久。美国食品药品监督局（FDA)已经批准了多个类似药物用于治疗黑色素瘤，其中一个名为nivolumab的药物更是在上周成为第一个被批准用于肺癌治疗。但是免疫检查点抑制剂（checkpoint inhibitors）例如PD-1抑制剂只对一部分人有效：只有20-30%的肺癌病人在经过治疗后，肿瘤缩小了。问题出在哪儿呢？ 有一种假说认为免疫检查点抑制剂对于那些有着更多突变的肿瘤更为有效。这些突变可能不会导致肿瘤细胞在体内疯狂扩增和转移，它们却可能编码了一些异常的冗余蛋白。这些冗余蛋白却可能是我们免疫治疗的关键靶点，因为它们提供了异物抗原，触发了我们的免疫系统去响应这些异物抗原。因而这种假说认为，病人肿瘤上的突变越多，那么所谓的这些新生的异物抗原也就越多，也就越能刺激病人的T细胞发挥作用，进而这些免疫检查点抑制剂药物也才可能有用武之地。 最近的一些研究证明了上述观点，携带有更多编码异物抗原突变的黑色素瘤病人也更能响应一种能够阻断名叫CTLA-4蛋白的免疫检查点抑制剂药物。现在，纽约一家癌症研究所的研究人员给接受了PD-1抑制剂治疗的34位非小细胞肺癌患者进行肿瘤外显子测序。他们发现病人携带的突变越多，异常蛋白也就越多，PD-1抑制剂对他们也就更有效果。例如，接受PD-1抑制剂治疗以后，72%携带有超过178个基因突变的病人相比于8%携带有更少突变的病人，延长了半年以上的寿命。此外因为吸烟造成特异基因突变的病人相比于那些不吸烟的肺癌病人更能够响应PD-1抑制剂的治疗。相关研究发表在最近的《科学》杂志上。 来自于约翰霍普金斯大学药学院的Drew Pardoll教授评价道，找到基因突变和癌症药物治疗效果之间的关联是非常吸引人的。尽管我们也不能下结论说PD-1抑制剂对那些不吸烟的肺癌患者没用，但是对肿瘤组织的基因测序可能会帮助肿瘤医师更好的对症下药。这项研究也启发我们，PD-1抑制剂可能对于吸烟造成的其他诸如食道癌之类的癌症会有更好的治疗效果。目前研究者还尝试把突变肿瘤表达的新生抗原做成个性化的肿瘤疫苗，进一步刺激病人的免疫系统产生积极反应。耶鲁大学的肺癌研究专家Roy Herbst也觉得免疫治疗癌症的潜力是巨大的。xyB882Ra 大鼠成纤维细胞生长因子10(FGF10)ELISA试剂盒xyB886Ra 大鼠血管紧张素转化酶2(AHZ2)ELISA试剂盒xyB343Ra 大鼠Bcl2关联X蛋白(Bax)ELISA试剂盒xyA049Ra 大鼠干扰素γ(IFNγ)ELISA试剂盒xyA050Ra 大鼠胰岛素样生长因子1(IGF1)ELISA试剂盒xyA051Ra 大鼠胰岛素样生长因子2(IGF2)ELISA试剂盒xyA056Ra 大鼠白介素10(IL10)ELISA试剂盒xyA064Ra 大鼠白介素18(IL18)ELISA试剂盒xyA071Ra 大鼠白介素1α(IL1α)ELISA试剂盒xyA073Ra 大鼠白介素2(IL2)ELISA试剂盒xyA077Ra 大鼠白介素4(IL4)ELISA试剂盒xyA079Ra 大鼠白介素6(IL6)ELISA试剂盒xyA463Ra 大鼠促甲状腺素(TSH)ELISA试剂盒xyA618Ra 大鼠速激肽受体2(TACR2)ELISA试剂盒xyA785Ra 大鼠肾损伤分子1(Kim1)ELISA试剂盒xyA721Ra 大鼠C-型钠尿肽(CNP)ELISA试剂盒xyC921Ra 大鼠血小板衍生生长因子B(PDGFB)ELISA试剂盒xyA105Ra 大鼠神经生长因子(NGF)ELISA试剂盒xyA106Ra 大鼠神经营养因子3(NT3)ELISA试剂盒xyA107Ra 大鼠神经营养因子4(NT4)ELISA试剂盒xyA113Ra 大鼠核糖核酸酶T2(RNAHZT2)ELISA试剂盒xyA120Ra 大鼠干细胞因子(SCF)ELISA试剂盒xyA124Ra 大鼠转化生长因子β1(TGFβ1)ELISA试剂盒xyA133Ra 大鼠肿瘤坏死因子α(TNFα)ELISA试剂盒xyA143Ra 大鼠血管内皮生长因子A(VEGFA)ELISA试剂盒xyA214Ra 大鼠窖蛋白(CAV1)ELISA试剂盒xyA222Ra 大鼠干扰素β(IFNβ)ELISA试剂盒xyA230Ra 大鼠丝氨酸蛋白酶1(PRSS1)ELISA试剂盒xyA441Ra 大鼠黄体激素(LH)ELISA试剂盒xyA447Ra 大鼠C-肽(C-Peptide)ELISA试剂盒xyA448Ra 大鼠胰岛素(INS)ELISA试剂盒xyA451Ra 大鼠激肽释放酶5(KLK5)ELISA试剂盒xyA569Ra 大鼠P选择素(HZLP)ELISA试剂盒xyA570Ra 大鼠Ⅰ型前胶原羧基端原肽(PⅠCP)ELISA试剂盒