Human IL-22BP ELISA kit
2015-10-09 10:12
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资料摘要:
Human IL-22BP
FOR RESEARCH USE ONLY
Assay range:3pg/ml-100pg/ml 96 determinations
Purpose
This kit allows for the determination of IL-22BP concentrations in Human serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Human IL-22BP level in the sample, use Purified Human IL-22BP antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-22BP to wells, Combined IL-22BP antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human IL-22BP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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