Mouse CaSR ELISA kit
2015-10-09 10:02
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资料摘要:
Mouse CaSR
FOR RESEARCH USE ONLY
Assay range:10pg/mL -360 pg/mL 96 determinations
Purpose
This kit allows for the determination of CaSR concentrations in Mouse Hippocampal tissue and other biological fluids.
Principle of the assay
The kit assay Mouse CaSR level in the sample, use Purified Mouse CaSR antibody to coat microtiter plate wells, make solid-phase antibody, then add CaSR to wells, Combined CaSR antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse CaSR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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小鼠钙敏感受体(CaSR 英文说明书.doc
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