鸡γ干扰素(IFN-γ)ELISA试剂盒
2015-09-10 16:12
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资料摘要:
Chicken IFN-γ
FOR RESEARCH USE ONLY
Assay range:1.5ng/L - 48ng/L 96 determinations
Purpose
This kit allows for the determination of IFN-γ concentrations in Chicken serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Chicken IFN-γ level in the sample,use Purified Chicken IFN-γ antibody to coat microtiter plate wells, make solid-phase antibody, then add IFN-γ to wells, Combined IFN-γ antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Chicken IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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鸡γ干扰素 IFN-γ 英文说明书.doc
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