Hormone
2014-04-22 09:14
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资料摘要:
Hormone
FOR RESEARCH USE ONLY
96 determinations
Purpose
This kit allows for the determination of Hormone expression in serum, and other biological fluids.
Principle of the assay
The kit assay Hormone level in the sample,use Purified Hormone antibody to coat microtiter plate wells, make solid-phase antibody, then add Hormone to wells, Combined With Hormone, after washing and removing non-combinative antibody and other components ,then Combined Hormone antibody which with HRP labeled become antibody – antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge Hormone exist in the sample or not.
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