Bat MERS Ab
2014-04-22 09:09
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资料摘要:
Bat MERS Ab
FOR RESEARCH USE ONLY
96 determinations
Purpose
This kit allows for the determination of MERS Ab expression in Bat serum, and other biological fluids.
Principle of the assay
The kit assay MERS Ab level in the sample, use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add MERS Ab to wells, Combined With MERS Ab, after washing and removing non-combinative antibody and other components ,then Combined antigen which with HRP labeled become antigen–antibody-enzyme-antigen complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge MERS Ab exist in the sample or not.
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