Human Kim-1
2014-04-21 17:21
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资料摘要:
Human Kim-1
FOR RESEARCH USE ONLY
Assay range:5ng/L - 160ng/L 96 determinations
Purpose
This kit allows for the determination of Kim-1 concentrations in Human serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Human Kim-1 level in the sample,use Purified Human Kim-1 antibody to coat microtiter plate wells, make solid-phase antibody, then add Kim-1 to wells, Combined Kim-1 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Kim-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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大鼠肾损伤分子1im1英文说明书.doc
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