Rat BMPs
2014-04-21 16:02
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资料摘要:
Rat BMPs
FOR RESEARCH USE ONLY
Assay range:0.3μg/L -12μg/L 96 determinations
Purpose
This kit allows for the determination of BMPs concentrations in Rat serum, cell
culture supernates and other biological fluids
Principle of the assay
The kit assay Rat BMPs level in the sample, use Purified Rat BMPs antibody to coat
microtiter plate wells, make solid-phase antibody, then add BMPs to wells, Combined BMPs
antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after
washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of Rat BMPs in the samples is then determined by comparing the O.D. of the
samples to the standard curve.
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