Rat interleukin 18(IL-18)
2014-04-21 12:34
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资料摘要:
Rat interleukin 18(IL-18)
FOR RESEARCH USE ONLY
Assay range:3ng/L -160 ng/L 96 determinations
Purpose
This kit allows for the determination of IL-18 concentrations in Rat serum, cell
culture supernates and other biological fluids
Principle of the assay
The kit assay Rat IL-18 level in the sample,use Purified Rat IL-18 antibody to coat
microtiter plate wells, make solid-phase antibody, then add IL-18 to wells, Combined IL-18
antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after
washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of Rat IL-18 in the samples is then determined by comparing the O.D. of the
samples to the standard curve.
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