Rat TNF-α
2014-04-21 12:16
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资料摘要:
Rat TNF-α
FOR RESEARCH USE ONLY
Assay range:10ng/L-360ng/L 96 determinations
Purpose
This kit allows for the determination of TNF-α concentrations in Rat serum, cell
culture supernates and other biological fluids
Principle of the assay
The kit assay Rat TNF-α level in the sample,use Purified Rat TNF-α antibody to coat
microtiter plate wells, make solid-phase antibody, then add TNF-αto wells, Combined
TNF-αantibody which With HRP labeled goat anti-Rat become antibody - antigen -
enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB
substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition
of a sulphuric acid solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm. The concentration of Rat TNF-αin the samples is then determined by
comparing the O.D. of the samples to the standard curve.
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