Rabies virus IgG(RV IgG)
2014-04-17 18:10
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资料摘要:
Rabies virus IgG(RV IgG)
FOR RESEARCH USE ONLY
Assay range: 5 IU/L - 180 IU/L 96 determinations
Purpose
This kit allows for the determination of RV IgG concentrations in serum, blood plasma, and other biological fluids
Principle of the assay
The kit assay RV IgG level in the sample, use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add RV IgG to wells, Combined antigen which With HRP labeled, become antigen - antibody - enzyme-antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RV IgG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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