Bovine mycoplasma Ab
2014-04-17 18:08
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资料摘要:
Bovine mycoplasma Ab
FOR RESEARCH USE ONLY
96 determinations
Purpose
This kit allows for the determination of mycoplasma Ab expression in Bovine serum, and other biological fluids.
Principle of the assay
The kit assay mycoplasma Ab level in the sample,use Purified mycoplasma antigen to coat microtiter plate wells, make solid-phase antigen, then add mycoplasma Ab to wells, Combined With mycoplasma, after washing and removing non-combinative antibody and other components ,then Combined mycoplasma antigen which with HRP labeled become antigen – antibody - enzyme- antigen complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge mycoplasma Ab exist in the sample or not.
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