Goat anti- Monkey Cyclooxygenase-1

Goat anti- Monkey Cyclooxygenase-1 Collect Sample – serum or blood plasma Storage: 2 - 8 °C Package size: 96 determinations PRINCIPLE OF THE METHOD The COX-1 kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known COX-1 concentrations and unknowns are pipetted into these wells. During the first incubation, the COX-1 antigen and a biotinylated monoclonal antibody specific for COX-1 are simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of COX-1 present in the samples. REAGENTS PROVIDED AND RECONSTTTUTION REAGENTS（Store at 2-8℃） 1×96 WELLS 0.5×96 WELLS RECONSTTTUTION 96/48-wells microtiter plates 1 0.5 Ready-to-use Plastiv cover 2 1 Ready-to-use Standard: 800IU/L 1Vials (0.6ml) 0.5Vials (0.3ml) See reagents preparation on page 3 Blank control 1Vials (1.0ml) 1Vials (0.5ml) Ready-to-use Standard Diluent 1Vials (5ml) 1Vials (2.5ml) Ready-to-use Biotinylated anti-COX-1 1Vials (6ml) 1Vials (3.0ml) Ready-to-use Streptavidin-HRP 1Vials (10ml) 1Vials (5.0ml) Ready-to-use Washing Buffer 1Vials (20ml) 1Vials (10ml) 50× concentrate Substrate A 1Vials (6.0ml) 1Vials (3.0ml) Ready-to-use Substrate B 1Vials （6.0ml) 1Vials (3.0ml) Ready-to-use Stopping Solution 1Vials (6.0ml) 1Vials (3.0ml) Ready-to-use Sample Diluent 1Vials (12ml) 1Vials (6.0ml) Ready-to-use