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核心参数
规格： 1次/盒
货号： 4319003
这是个完整强大的mRNA扩增试剂盒mRNA amplification kit ，优化后可以使用50ng至1微克的总RNA作为起始材料。该试剂盒可以生成5至50微克放大的mRNA用于标记。并可以与总RNA提取试剂盒以及氨基烯丙基标记试剂盒连用。
Needed Equipement
? Ethanol 100%
? Ethanol 70%
? Ice for incubation
? PCR tubes
? Thermal cycler
? Refrigerated microfuge capable of 10,000 rpm or more
? Micropipettors
? Vortex mixer
? Homogenizer (if using tissue)
? Temperature regulated water bath or dry bath
Kit Contents
| Items | Packaging | Storage |
| Enzyme Mix 1 | 16.5μl in 0.5ml tube | -20°C Freezer |
| RT Booster (enhancer solution) | 50μl in 0.5ml tube | -20°C Freezer |
| Reaction buffer 1 | 50μl in 0.5ml tube | -20°C Freezer |
| dNTP Mix | 40μl in 0.5ml tube | -20°C Freezer |
| DTT | 50μl in 0.5ml tube | -20°C Freezer |
| Primer 1 Mix | 11μl in 0.5ml tube | -20°C Freezer |
| Primer 2 | 11μl in 0.5ml tube | -20°C Freezer |
| Primer 3 | 22μl in 0.5ml tube | -20°C Freezer |
| Primer 4 | 11μl in 0.5ml tube | -20°C Freezer |
| Enzyme Mix 2 | 11μl in 0.5ml tube | -20°C Freezer |
| Reaction Buffer 2 | 150μl in 0.5ml tube | -20°C Freezer |
| DNA Binding Buffer | 1.1ml in 2ml tube | Room Temp |
| DNA Wash Buffer Concentrate | 0.5ml in 8ml bottle | Room Temp |
| DNA Micro Column | 10 | Room Temp |
| Elution Tube (1.5ml) | 10 | Room Temp |
| 2 ml Wash Tube | 10 | Room Temp |
| Nuclease Free Water | 1.5ml in 2ml tube | -20°C Freezer |
| Enzyme Mix 3 | 22μl in 0.5ml tube | -20°C Freezer |
| NTP Mix | 79.2μl in 0.5ml tube | -20°C Freezer |
| Reaction Buffer 3 | 22μl in 0.5ml tube | -20°C Freezer |
| DNAse | 11μl in 0.5ml tube | -20°C Freezer |
| Sodium Acetate Solution | 50μl in 0.5ml tube | -20°C Freezer |
| Linear Acrylamide | 20μl in 0.5ml tube | -20°C Freezer |
Short Protocol
1. Spin all tubes 5 to 10 s before use.
2. Perform first strand cDNA synthesis.
3. Perform second strand cDNA synthesis.
4. Amplify the double-stranded (ds) cDNA.
5. Purify the double-stranded cDNA.
6. Transcribe the ds cDNA with T7 RNA polymerase in vitro.
7. Purify the amplified RNA (aRNA).
8. Perform cDNA synthesis on aRNA using aminoallyl-labeled dNTPs.
Please contact us for further information about the complete protocol.
| 编号 | 名称 |
| MAK | 迷你(mini)Amp mRNA 扩增试剂盒(10次反应) |
企业名称
孚光精仪（香港）有限公司
企业信息已认证
企业类型
信用代码
成立日期
2008-09-30
注册资本
100
经营范围
孚光精仪（中国）有限公司
公司地址
上海黄浦区
客服电话
仪器信息网APP
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