产品名称: 大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
英文名称: Rat Alpha-fetoprotein,AFP ELISA Kit
货号: CSB-E08281r
规格: 96T
种属: Rat
待测物名称: alpha-fetoprotein
缩写: AFP
样本类型: serum, plasma, cell culture supernates, tissue homogenates
检测范围: 15.6 pg/ml-1000 pg/ml
灵敏度: 3.9 pg/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
用途: For research use only. Not for diagnostic use.
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP bound in the initial step. The color development is stopped and the intensity of the color is measured.
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
特异性: This assay has high sensitivity and excellent specificity for detection of Rat AFP. No significant cross-reactivity or interference between Rat AFP and analogues was observed.
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the AFP concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
产品名称 英文名称 货号 规格
小鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit Mouse Alpha-fetoprotein,AFP ELISA Kit CSB-E08282m 96T
Pig Alpha-fetoprotein(AFP) ELISA kit Pig Alpha-fetoprotein(AFP) ELISA kit CSB-EL001421PI 96T
裸鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit Nude mouse Alpha-fetoprotein,AFP ELISA Kit CSB-E08934Nu 96T
人甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit Human Alpha-fetoprotein,AFP ELISA Kit CSB-E04770h 96T
Horse Alpha-fetoprotein(AFP) ELISA kit Horse Alpha-fetoprotein(AFP) ELISA kit CSB-EL001421HO 96T
Dog Alpha-fetoprotein(AFP) ELISA kit Dog Alpha-fetoprotein(AFP) ELISA kit CSB-EL001421DO 96T
Chicken Alpha-fetoprotein(AFP) ELISA kit Chicken Alpha-fetoprotein(AFP) ELISA kit CSB-EL001421CH 96T
Bovine Alpha-fetoprotein(AFP) ELISA kit Bovine Alpha-fetoprotein(AFP) ELISA kit CSB-EL001421BO 96T
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
抗体
产品名称 货号 规格
Rabbit anti-human alpha-fetoprotein polyclonal Antibody CSB-PA001421GA01HU 150ul
大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Bovine Alpha-fetoprotein(AFP) 1mg
Recombinant Pan troglodytes Alpha-fetoprotein(AFP) 1mg
Recombinant Dog Alpha-fetoprotein(AFP) 1mg
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Recombinant Penicillium chrysogenum Antifungal protein(afp) 1mg
Recombinant Penicillium chrysogenum Antifungal protein(afp) 1mg
Recombinant Antifungal protein(afp) 1mg
Recombinant Gorilla gorilla gorilla Alpha-fetoprotein(AFP) 1mg
Recombinant Mouse Alpha-fetoprotein(Afp) 1mg
Recombinant Rat Alpha-fetoprotein(Afp) 1mg
Recombinant Human Alpha-fetoprotein(AFP) 1mg
Recombinant Horse Alpha-fetoprotein(AFP) 1mg
Recombinant Chicken Alpha-fetoprotein(AFP) 1mg
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人甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit第三方验证报告
产品类型:ELISA Kit 产品名称:人甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit 英文名称:Human Alpha-fetoprotein,AFP ELISA Kit 货号:CSB-E04770h 规格: 96T 种属: Human 其他种属:Bovine Chicken Dog Horse Mouse Nude mouse Pig Rat 待测物名称: alpha-fetoprotein 缩写: AFP 检测范围: 1.56 ng/ml-100 ng/ml 灵敏度: 1.33 ng/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm
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2014/09/02
人甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit说明书
产品类型:ELISA Kit 产品名称:人甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit 英文名称:Human Alpha-fetoprotein,AFP ELISA Kit 货号:CSB-E04770h 规格: 96T 种属: Human 其他种属:Bovine Chicken Dog Horse Mouse Nude mouse Pig Rat 待测物名称: alpha-fetoprotein 缩写: AFP 检测范围: 1.56 ng/ml-100 ng/ml 灵敏度: 1.33 ng/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm
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2014/09/02
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来自Ludwig癌症研究所的研究人员揭示了一种现象,其有可能改变人们关于基因组如何表达从而生成及维持哺乳动物生命的一个普遍观点。这篇发表在《科学》(Science)杂志上的论文,帮助解释了为何基因相同的动物有时在生物学和外形上会如此的不同,以及为何由共同的一组异常基因引起的一些遗传疾病在不同个体身上疾病严重程度显示如此大的差异。 “我们捕获了此前从未描述过的、基因表达水平的固有随机性——其持续存在于整个发育时期并进入到成年期,”瑞典Ludwig癌症研究所和Karolinska学院的Rickard Sandberg说。采用Sandberg实验室开发的一种强有力的新技术分析单细胞中整体的基因表达,从而生成了这一研究发现。
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上海希美化学有限公司
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310228001203103
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