重组人Dickkopf相关蛋白1(Recombinant Human DKK1)

DKK1 Human
Dickkopf-Related Protein 1 Human Recombinant
Catalog No. PRO-673

Synonyms:
DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK.

Introduction:
DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions and takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid and is significantly involved in the development of tau phosphorylation.
DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer.

Description:
DKK1 produced in Hi-5 is a single, glycosylated polypeptide chain containing 253 amino acids (32-266 a.a.) and having a molecular mass of 27.8 kDa.
DKK1 is expressed with a 15 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Source:
Hi-5 Baculovirus

Physical Appearance:
Sterile filtered colorless solution.

Formulation:
The DKK1 protein solution contains 20mM Tris-HCl, pH-8, 1mM EDTA,0.1M NaCl, 1mM DTT and 10% Glycerol.

Stability:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 85.0% as determined by SDS-PAGE.

Amino Acid Sequence:
ADPTLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRHSG RLVPRGSHHH HHH.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.