14-3-3 b/a(C-term)(YWHAB)antibody

14-3-3 b/a(C-term)(YWHAB)antibody
Cat.#: 1062-1
Rabbit Monoclonal Antibody
Clone ID: Y62
Swiss Prot: P31946
Mol Weight: 30kDa
Size: 100ul

Description
The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3).

Recommended Applications
WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:1,000
IHC: 1:500
ICC: 1:500
IP: 1:40
FC: 1:40

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data

Specificity
A synthetic peptide corresponding to C-terminal residues of human 14-3-3 b/a was used as immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
YWHAB antibody, GW128 antibody, HS1 antibody, KCIP-1 antibody, YWHAA antibody, 14-3-3 protein beta/alpha, N-terminally processed antibody, Protein 1054 antibody, Protein kinase C inhibitor protein 1 antibody

Description References
1. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617
2. Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889
3. Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619