葡萄球菌的振荡培养

一种模型生物膜产生菌株，表皮葡萄球菌RP62A的胞外含碳水化合物的聚合物Extracellular carbohydrate-containing polymers of a model biofilm-producing strain, Staphylococcus epidermidis RP62A

Bacterial strains and culture conditions. The S. epidermidis RP62A (ATCC 35984) and S. aureus MN8m strains were kindly provided by Gerald Pier (Harvard Medical School, Boston, MA). Cells were grown at 37°C for 24 h under TABLE 1. N-Acetylglucosamine-containing polysaccharides of biofilm-positive staphylococcal strains Polysaccharide Strain Analytical artifact Reference(s) PS/A S. epidermidis RP62A 45 PS/A, PNSG S. carnosus(pCN27) N-Succinyl substitution 38 S. aureus MN8m 39, 40 PS/A, PNAG S. aureus MN8m 36 PIA S. epidermidis RP62A 33 S. epidermidis 1457 SAA S. epidermidis RP62A Presence of glucose 6 S. epidermidis clinical isolates 1 SAE S. aureus MN8m 25 3008 SADOVSKAYA ET AL. INFECT. IMMUN.different conditions of shaking and aeration, as follows: (i) in 1 liter of tryptic soy broth (TSB) (Becton Dickinson, Le Pont de Claix, France) or brain heart infusion broth (BHI) (Becton, Dickinson, Le Pont de Claix, France) supplemented or not supplemented with glucose (1%, wt/vol) in a 3-liter Erlenmeyer flask with vigorous shaking (aerobic onditions, 80 strokes/min; Gerhard Laboshake shaker; Fisher Scientific, Elancourt, France); (ii) in 3 liters of TSB or BHI supplemented or not supplemented with glucose (1%, wt/vol), in a 5-liter Erlenmeyer flask with moderate shaking (aero-anaerobic conditions, 60 strokes/min);