蛋白酶K

Catalog Number EN010 

Product Name Proteinase K Protein

Alias Endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, protease K, Tritirachium album proteinase K 

Size 1g-1kg, bulk 

Species Engyodontium album 

Expression Host Yeast 

CAS Number 39450-01-6 

Applications Genomic DNA extraction, Enzyme digestion and removal

Source This product is derived from yeast cells expressing Proteinase K gene of Engyodontium album. Purity RNase and DNase free. 

Endotoxin None detected

Cross-Reactivity No cross-reactivity with other antigens or proteins.

Buffer Dilution Buffer: 20mM Tris-HCl, pH 7.4; Storage buffer: 20mM Tris-HCl, 50% Glycerol, pH 7.4.

pH Range 4.0-12.0 (Optimum PH 7.5-9.0) 

Temperature Range 37-70°C

Specific Activity ≥ 30 units/mg 

Molecular Weight 29.3 kDa 

Background Proteinase K is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album). Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. 

Storage Lyophilized powder: store at 4 °C; Liquid: store at -20 °C. 

Shipping Condition Transport at room temperature 

Stability Lyophilized powder: store at -20 °C for 3 years; Liquid: maintain stable at ambient temperature for 12 months. 

For Research Use Only!