应用级别: 实验室级别
仪器种类: 中低压制备液相色谱
流速范围: 50-500mL/min
流量精度: RSD≤0.5%
流量重现性: 1%
最大耐压: 145-200psi
波长范围: ±1nm
波长重现性: 1nm
基线噪声: 3×10-5AU
采集频率: 100
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Biotage Isolera 系列
Flash快速制备液相色谱
耐士科技——Biotage中国区总代理
耐士科技以最优质的服务提供Biotage全系产品。Biotage Isolera 系列是世界上最智能的快速纯化系统,它拥有自己独创的智能参数设置,共有3个系统,多种配置可选。可以让化学家们轻松地完成对从mg级到150g以上样品 的更好的分离。创新的TLC-to-gradient专利技术可以根据薄层层析色谱的数据自动产生适合样品的溶剂洗脱梯度,并建议适合该样品量的色谱柱。
通过双波长检测收集馏分,最多可以在单一梯度下同时使用四种溶剂进行洗脱,以达到最大限度提高纯度和收率的目的。
通过梯度优化功能,可以实现加大上样量同 时减少溶剂使用量。
耐士科技Biotage Isolera LS
Flash快速制备液相色谱
单样品分离从毫克级到百克级
内置进样泵,提高样品加载的安全性
可用500g的干法上样柱,提高分离性能
可用funnel rack,馏分收集能力可达到320L
软件系统同Isolera One和Isolera Four,易于上手,占用体积小
流速50-500mL/min
耐士科技Biotage Isolera One
Flash快速制备液相色谱主要特点
在单一系统上实现毫克级到百克级的放大
Biotage Isolera LS快速分离系统显著缩短了分离大量样品的时间,流速可达到每分钟50-500mL,只需简单地选择或创建分离方法,加载分离样品,然后运行就可实现。新 增高级高能包括:溶剂节约功能,梯度优化功能(GO),旁路接受馏分功能,等梯度保持功能,远程编辑功能,双波长接受馏分,在单一梯度中最多可实现使用四 种溶剂,可加入第三种溶剂等同于共溶剂,配备紫外-可见光检测器,检测波长在200-800nm。
通过最大流速每分钟500mL的泵加速大量样品的分离能力
每分钟50-500mL的流速显著缩短分离周期。当Isolera LS配备SNAP 15oog色谱柱,一个150g的样品可快速完成分离,大大提高化学家们的工作效率。
通过独特的上样泵安全注射大量样品
Isolera LS通过内置的蠕动泵克服了手动注射的缺点,例如漏液或溢出。含氟聚合物的管路耐腐蚀,溶出少,可实现液体样品通过泵直接注射到SNAP 750g或者SNAP 1500g色谱柱中。
通过DLV-500提高分离纯化效率
对于需要预吸附到固体负载物的样品,500g容量的空上样柱可供选择,这个空上样柱提供一个可调节的底座,最少可调节到100g的样品,直接装备到Isolera LS上,节约宝贵空间。
通过最大的320-L收集瓶支架确保足够的馏分收集能力
Biotage Isolera LS的常规收集能力为9.6L。附加收集能力最高可升级到320L,有收集瓶支架单元可供选择。收集瓶支架单元由2组支架(每组有16个收集瓶)收集瓶,漏液检测器和1个装有轮子的手推车(用于支撑这个系统)组成。
通过SNAP 750和1500g色谱柱纯化75g到超过150g的样品
SNAP 750和1500g色谱柱通过提供最高的样品负载量,最快的产出,最优化的放大分离区实现效能最大化。
用户单位 | 采购时间 |
---|---|
南京大学 | 2012-10-16 |
先声药业 | 2012-09-10 |
药明康德 | 2010-12-14 |
For the purpose of synthesis of tyrosol -d-glucopyranoside (salidroside) and its -d- and -d-galactopyranoside analogues, transglycosylation of tyrosol with fungal glycosidases (from Aspergillusniger and Aspergillus oryzae) was executed. Cellobiose, lactose and melibiose served as glycosyl donor giv-ing 6.7% yield of salidroside, 10.9% of tyrosol -d-galactopyranoside, and 32.2% of -d galactopyranoside.The glycosylations proceeded on the primary hydroxyl of the tyrosol.
In this work we reported the generation of D-proline-derived hydroxamic acids as inhibitors of anthrax lethal factor (LF), taking advantage of a pyrrolidine ring as the central scaffold and a hydroxamate group as the Zn2t chelating agent. The introduction of two hydrophobic groups addressing the S10 subsite and a long substrate-binding groove was conceived by overlapping the bioactive conformations of two reported LF inhibitors. Micromolar affinity of compound 38 suggested cis-3-substituted-1-sulfonamido-Dproline hydroxamic acids as a promising class of peptidomimetic inhibitors for developing novel LF inhibitors.
The Hedgehog (Hh) signaling pathway plays critical roles in metazoan development and in cancer. How the Hh ligand is secreted and spreads to distant cells is unclear, given its covalent modification with a hydrophobic cholesterol molecule, which makes it stick to membranes. We demonstrate that Hh ligand secretion from vertebrate cells is accomplished via two distinct and synergistic cholesteroldependent binding events, mediated by two proteins that are essential for vertebrate Hh signaling: the membrane protein Dispatched (Disp) and a member of the Scube family of secreted proteins. Cholesterol modification is sufficient for a heterologous protein to interact with Scube and to be secreted in a Scube-dependent manner. Disp and Scube recognize different structural aspects of cholesterol similarly to how Niemann-Pick disease proteins 1 and 2 interact with cholesterol, suggesting a hand-off mechanism for transferring Hh from Disp to Scube. Thus, Disp and Scube cooperate to dramatically enhance the secretion and solubility of the cholesterol-modified Hh ligand.
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