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德国耶拿 multi N/C 3100 TOC总有机碳/总氮分析仪型号： multi N/C 3100 -
瑞士万通Multi Autolab/M204多通道电化学工作站型号： Multi Autolab/M204 -
德国FRITSCH P7 小型行星式球磨机/仪加强型型号： PULVERISETTE 7 premium line -
激光干涉仪型号： XL80 -
Hitachi日立CT15RE台式离心机型号： CT15RE -
Hitachi日立超速离心机CP70ME型号： CP70ME -
电热鼓风干燥箱型号： CS101-ABN -
Agilent 8890 气相色谱系统型号： 8890 GC -
日立Primaide PLUS高效液相色谱仪型号： Primaide PLUS
获取电话平台编号：Bio-105113
Candida broadrunensis Suh et Zhou 拉丁名
ATCC MYA-4650 编号
Strain Designations 菌种别名 EH019 [CBS 11838]
Application Ferments cellobiose
Biosafety Level 安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions 培养条件
Frozen 冷冻物 : -80°C or colder
Freeze-Dried 冻干物 : 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌株 yes
Preceptrol no
Comments 注释 Insect associated yeast
Morphology After 10 days in YM broth at 25°C, cells are subglobose to ellipsoidal (2-4 x 2-4 ?m) and occur singly, in pairs or in small clusters. Pseudohyphae and true hyphae are not present. After 10 days on YM agar at 25°C, colonies are white to cream-colored, butyrous and smooth with entire margins.
Medium 培养基 ATCC Medium 28: Emmons' modification of Sabouraud's agar
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1245: YEPD
Growth Conditions 生长条件
Temperature 培养温度 : 20°C to 25°C
Atmosphere 需氧情况 : Typical aerobic
Sequenced Data
ITS DNA sequence of MYA-4650:
TGATTTGAGGTCAAACTTGTTTGTTGTTTTGTAAGGCAAAGCCAAATATACCAAAAATTCACTAACTGGAATTCTCAACGAGTTGGATAAACCTAATACATTGAAAATGTTCCTGAAAGCACTATTTAGTACGACTCGTGCCAATACTTTTCAAGCAAACGCCTAGTTCGACTAAGAGTATCACTCAATACCAAACCCGAAGGTTTGAGAGAGAAATGACGCTCAAACAGGCATGCCCTTTGGAATACCAAAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGAAAATCTGCAATTCATATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTTTGAAGATTTTTGAATTTAATCAACAAATTGACAAATAATAAATAAACAATTCAATTAAAAATTGAAGTTTTAAACCTCTGGCCCAACTTATTTCTAAGCCAGACCAAAGCAAAAGTTTCTAATAAAGAAAAACATTGTGTGTAAGTTTGTTTACCGCGCAATTAAGCGCAGGT
D1D2 region of the 26S ribosomal RNA gene:
ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGTCTTAGTAACGGCGAGTGAAGCGACAAAAGCTCAAATTTGAAATCTGGCGCCTTCGGTGTCCGAGTTGTAATTTGAAGAAGGTAACTTTGGTTTTGGCTCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGCCCAATTCCATGTAAAGTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCTTGAGATCAGACTTGGTATTTTGTATGTCTTACTTTCGGGTGGGGCCTCTGCAGTTTACCGGGCCAGCATCAGTTTGGATGGTAGGATAATGACATTGGAATGTGGCTCTGCTTCGGTAGAGTGTTATAGCCCTTGTTGATACTGCCTGTCTAGACTGAGGACTGCGTCTTTGACTAGGATGCTGGCATAATGATCCTAAGCCAC
Morphology After 10 days in YM broth at 25°C, cells are subglobose to ellipsoidal (2-4 x 2-4 ?m) and occur singly, in pairs or in small clusters. Pseudohyphae and true hyphae are not present. After 10 days on YM agar at 25°C, colonies are white to cream-colored, butyrous and smooth with entire margins.
Name of Depositor 寄存者 SO Suh
Special Collection ATCC
Chain of Custody 来源国家 ATCC <-- SO Suh
Isolation 分离基物
Gut of unidentified tenebrionid beetle from rotten log, Bull Run Mt., Broad Run, Virginia, USA.
Cross References
Nucleotide (GenBank) : HQ651969 D1/D2 region of 28S rRNA gene
Nucleotide (GenBank) : HQ652048 ITS including 5.8S rRNA gene
Nucleotide (GenBank) : HQ651934 18S rRNA gene
Nucleotide (GenBank) : KC507462 EF-1 alpha gene
Nucleotide (GenBank) : KC507422 RPB1 gene
Nucleotide (GenBank) : KC512847 RPB2 gene
Nucleotide (GenBank) : KC479715 XYL1 gene
References 参考文献
Suh SO, et al. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov., and Candida manassasensis sp. nov., four novel yeasts associated with wood- ingesting insects and their ecological and biofuel implications. Int. J. Syst. Evol. Microbiol. 63: 4330-4339, 2013. PubMed: 24014624
