平台编号:Bio-105124
Cryptococcus gattii (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout 拉丁名
ATCC MYA-4093 编号
Deposited As Cryptococcus bacillisporus
Strain Designations 菌种别名 A1M R265 [CBS 10514]
Alternate State Filobasidiella bacillispora Kwon-Chung, teleomorph
Application 用途
Multigene phylogeny and phenotypic characterization.
Biomedical Research and Development Material
Biosafety Level 安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions 培养条件
Frozen 冷冻物 : -80°C or colder
Freeze-Dried 冻干物 : 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌株 no
Antigenic Properties Serotype B
Genotype VGIIa, AFLP6A
Preceptrol no
Genome Sequenced Strain
Yes
Mating Type alpha
Comments 注释
Genome sequencing strain (Broad Institute, USA)
Cause cryptococcosis
Represents one of the two genotypes causing an outbreak in Vancouver Island
Multigene phylogeny and phenotypic characterization
Designated as type strain of Cryptococcus deuterogattii Hagen & Boekhout by Hagen et al. (2015)
Medium 培养基 ATCC Medium 28: Emmons' modification of Sabouraud's agar
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1245: YEPD
Growth Conditions 生长条件
Temperature 培养温度 : 24°C to 26°C
Atmosphere 需氧情况 : Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATACTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCAGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA
D1D2 region of the 26S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC
26S ribosomal RNA gene and intergenic spacer (IGS), partial sequence
ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAATATGTTAGGTCTCGGGGGGCTTCCTCTAGAGACTTGGATGTAAGAGGCTTTGCTTTTTTTTATGACGATGACTTGTCAAAGTGTGGATGACTCCAGGAGAGAGGTCGGGCTGGTAATTAGTAATATCAGTCAGTCATTTCAGCTGGCGCCATCGATACTTTATAAGTCAAACTTAACCATTTCACTGTGTAGTAAGTAGTAGGCTCTGAATTACTAGAGACACTTGCCAGGTTATCGCAAGTTGGCAGGCAGGCAGGACACACATACTATTGATTTCACGGTCATGGGGGACTTGGCTGCTGGTGCTTGAGTTGCATAATAGAGAGAGTATATGCATGTCGCGGGGGGGGGACTTGGCTAAGATGCGTTATGCGGCAAGCAAGGTAAGTAGTATTTGTATTAAAGTATTTAGTTACTTTTCCAGTCATGGGGGACTTTGATAGTGGTTGTGAGCCGGAATGAAAAATGGGTAAATGTGGTATGGATGGTGAGAGGGGGAGCTGCCAAGTGTATGCAGTGGGTGTGCTTATAGCATAGTCAAGAGGGGATGGGCAATTAAATTTTGGAAAGTTGTTTGACCCGACCTGACGGTGACTCTAATAGGCTGGTCAAGCAAACGTTTAAGTTGAGTCAAGCTCGGTCAAGCAAAGTCTAGAAAAGATTAATGATCGCTAAGAATTACTCCGGTCGCGGGGGGCTTGCAACTTGTCTGGCCCACTGTGGGTCCAGTTT
glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds
TACTTGCATAAATACAATGGTTGTCAAGGTTGGAATCAACGGTTTCGGTACGTGATCTGTCTCTATCAAGCGTTGCTTCATATGTCACCAATGTGGTTTCCGATATTGTGCACCGTCCTTGGGTTACTGGTAGCTAATAGTTATGCGATAGGTCGTATCGGTCGAATTGTTCTCAGGTATGCTTTAAGTCAAGGGGTAAGACAGCTGCGTATCTAACCATCAAATTAGGAACGCCATCGAGCATGGAGACCTTGAGGTTGTTGCTGTCAACGAGTGAGTATTCTCAAAAGTGTTAATCTATTAAATAAATTATTTACTTTTGCAAAGCCCTTTCATTGACTTGGACTACATGGTATAATACTTTCTTCTGTACAATTGCTTCGTTTTGTGCTAACTGTAGCATGCGTAGGTTTACATGTTCAAGTACGACTCCGTAAGTCTCTGATGGCTCTAGCGCTCTATATGGCATTTTAACCTCATTCCCAACAGACACATGGTCGCTTCAAGGGTTCTGTCGAGGTTAAGGACGGTAAGCTCTATATCAACAACAAGGCCATTTCCGTTTTCGGTGAGAAGGACCCCGCCAACATCAAGTGGGGTGACGCTGGTGCCGAGTACATCGTTGAGTCTACCGGTGTCTTCACCACTACCGAAAAGGCCGGTGTCCAT
Name of Depositor 寄存者 L Hoang
Special Collection NSF - Mycology
Chain of Custody 来源国家 ATCC <-- L. Hoang
Isolation 分离基物
Bronchial alveolar lavage, Duncan, Vancouver Island, B.C. Canada.
Cross References
Nucleotide (GenBank) : AAFP00000000 Cryptococcus gattii VGII R265, whole genome shotgun sequencing project
References 参考文献
Hu G, Kronstad JW. Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition. Curr Genet 49: 341-350, 2006. PubMed: 16397763
Fraser James A, et al. Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak. Nature 437: 1360-1364, 2005.
Kidd Sarah E, et al. Comparative gene genealogies indicate that two clonal lineages of Cryptococcus gattii in British Columbia resemble strains from other geographical areas. Eukaryot. Cell 4: 1629-1638, 2005.
Kidd Sarah E, et al. A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada). Proc. Natl. Acad. Sci. USA 101: 17258-17263, 2004.
Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot Cell 8(3): 353-361, 2009. PubMed: 19151324
Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262
Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334
D'Souza CA, et al. Genome variation in Cryptococcus gattii, an emerging pathogen of immunocompetent hosts. mBio 2: e00342-10, 2011. PubMed: 21304167
Hagen F, et al. Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex. Fungal Genet. Biol. 78: 16-48, 2015. PubMed: 25721988