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Exophiala dermatitidis (Kano) de Hoog
28869 ™
An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
Product category Fungi
Strain designationCBS 207.35 [IFO 6421, IMI 93967, NBRC 6421, NCPF 2422]
Type strainYes
Isolation source Face
Geographical isolationJapan
Product format Frozen
Storage conditions-80°C or colder
Commentstaxonomy
Effect of carbon source on conidiogenesis
MediumATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 200: YM agar or YM broth
ATCC Medium 307: Cornmeal agar
Temperature24-26°C
AtmosphereAerobic
Handling procedureFrozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 4-6 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
