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Cunninghamella echinulata (Thaxter) Thaxter var. echinulata 拉丁名
ATCC 42616 编号
An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
Product category Fungi
Strain designation 别名 NRRL 1382
Type strain 模式菌株 No
Genome sequenced strain Yes
Applications Biofuel production
Product format 提供形式 Frozen
Storage conditions 保藏温度 -80°C or colder
Specific applications Potential biofuel production agent: biodiesel Preceptrol No
Comments 注释 Morphology Genome sequencing strain (Joint Genome Institute, Department of Energy, USA)
Medium 培养基 ATCC Medium 200: YM agar or YM broth
ATCC Medium 323: Malt agar medium
ATCC Medium 336: Potato dextrose agar (PDA)
Temperature 培养温度 24-26°C
Atmosphere 需氧情况 Aerobic
Handling procedure Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).
Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).
Immerse the ampoule just sufficient to cover the frozen material.
Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly.
The sign of viability is noticeable typically after 4-6 days of incubation.
However, the time necessary for significant growth will vary from strain to strain.
Deposited as Cunninghamella echinulata (Thaxter) Thaxter var. echinulata Depositors NRRL
