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Mycoplasma hyopneumoniae Mare and Switzer 拉丁名
ATCC 25617 编号
Product category Bacteria
Strain designation 别名 [NCTC 10127, V.P.P.11]
Type strain 模式菌株 No
Isolation 分离源 source Swine pneumonia
Applications 用途 Agricultural research Infectious disease research Respiratory disease research
Product format 提供形式 Freeze-dried
Storage conditions 保藏温度 2°C to 8°C
Specific applications Respiratory research
Preceptrol No
Comments Taxonomic status
Medium 培养基 ATCC Medium 1699: Revised Mycoplasma medium
Temperature 培养温度 37°C
Atmosphere 需氧情况 95% Air, 5% CO2
Handling procedure Follow instructions as suggested for the culturing of Mollicutes: PROCEDURES FOR PROPAGATING MOLLICUTES: Open the vial according to the enclosed instructions. Using a Pasteur or 1.0 mL pipette, withdraw approximately 0.5 to 1.0 mL from a tube containing 5.0 mL.
Rehydrate the pellet. Aseptically transfer this aliquot back into the tube. Mix well. Make serial dilutions by transferring 0.25 mL from the original tube to a tube containing 2.25 mL. Repeat process by transferring 0.25 mL from the second to a third tube, etc.
Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze drying process often inhibits growth.
Use an uninoculated tube of broth to serve as a control. Plates may be inoculated to check colonial morphology. You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony-forming units. However, not all strains do well on solid medium.
Incubate all tubes and plates under the recommended conditions and appropriate temperature. The time necessary for growth will vary from strain to strain.
Growth on plates generally requires additional incubation. Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both. Tubes are incubated under aerobic conditions. Plate are incubated under 5% CO2 conditions. The incubation temperature is 37°C. Incubate up to 14 days.
Handling notes This strain does not grow on agar. Store vials at freezer temperatures until ready to use. We have found that using a candle jar for CO2 conditions works better for those strains whose medium has an indicator present. CO2 incubators may lower the pH of the medium enough to cause a color change.
This change may make it difficult to observe growth with those strains that show little turbidity.Deposited as Mycoplasma hyopneumoniae Mare and Switzer Depositors RG Wittler
Chain of custody ATCC <-- RG Wittler <-- W.P. Switzer V.P.P. 11
Type of isolate Animal
