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Aspergillus niger van Tieghem
13497™
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Fungi
Strain designation
Armbruster C-14
Type strain
No
Genome sequenced strain
Yes
Isolation source
UV mutant derived from M 370, ATCC 13496
Applications
Antibiotic production
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Specific applications
produces glucoamylase
Preceptrol
No
Characteristics
Comments
Genome sequencing strain (Joint Genome Institute, Department of Energy, USA)
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
Handling information
Medium
ATCC Medium 200: YM agar or YM broth
ATCC Medium 325: Malt extract agar (Blakeslee's formula)
ATCC Medium 336: Potato dextrose agar (PDA)
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
