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平台编号:Bio-134600
Malassezia slooffiae J. Guillot, Midgley et E. Guého 拉丁名
38593™ 统一编号
An ampoule containing viable cells suspended in cryoprotectant.
Product category 产品类别 Fungi
Product type 产品类型 Yeast
Strain designation 别名 683
Type strain 模式菌种 No
Isolation source 隔离源 Scalp
Product format 规格 Freeze-dried
Storage conditions 保存条件 2°C to 8°C
Specific applications:Biomedical Research and Development Material
Preceptrol:No
Comments:Occurrence in children
Medium 培养基
ATCC Medium 1072: Pityrosporum medium
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 2737: Modified Leeming & Notman Agar (MLNA)
Temperature 培养温度 37°C
Atmosphere 需氧条件 Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
1 Open an ampoule according to enclosed instructions.
2 From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3 Aseptically transfer the suspension back into the test tube of sterile distilled water.
4 Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
5 Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
6 Incubate the inoculum at the propagation conditions recommended.
7 Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.