获取电话平台编号：Bio-134775
Human rhinovirus 37 拉丁名
VR-1607 编号
Product category Viruses
Product type Animal virus
Classification Picornaviridae, Rhinovirus, Human rhinovirus B
Strain designation 151-1
Genome sequenced strain Yes
Isolation source Presumed from human throat washings; this preparation has been derived from VR-1147 following treatment to remove mycoplasma.
Applications Infectious disease research
Respiratory disease research
Product format Frozen
Storage conditions -70°C or colder
Specific applicationsRespiratory research
CommentsThis product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-169-001-021 (ATCC VR-1147). It replaces VR-1147 and VR-510, which are no longer available. Strain 151-1 is the prototype strain of Human rhinovirus 37 and it has been fully sequenced (GenBank: EF173423.1).
Mycoplasma contaminationNot detectedHostH1HeLa (ATCC CRL-1958)
Alternate Hosts: WI-38 (ATCC CCL-75 ), HeLa (ATCC CCL-2 ), KB (ATCC CCL-17 ) and other human cells
Effectscell rounding; cell sloughing; CPE
Complete mediumEMEM (ATCC® 30-2003™) + 2% FBS (ATCC® 30-2020™)
Temperature33°C
Atmosphere95% Air, 5% CO2
IncubationIncubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired. Freeze preparation as rapidRecommendation for infectionFor best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired. Freeze preparation as rapidly as possible.Handling notes This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-169-001-021 (ATCC® VR-1147™). It replaces VR-1147™ and VR-510™, which are no longer available. Strain 151-1 is the prototype strain of Human rhinovirus 37 and this ATCC product has been fully sequenced. GenBank: EF173423.1
Key abbreviations°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
DepositorsNIAID, ATCCType of isolateHuman
