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Human herpesvirus 5
VR-3348™
Human herpesvirus 5 strain UL32-EGFP-HCMV-TB40 is propagated in MRC-5 cells (ATCC CCL-171). This strain was sourced from the in vitro recombination of HCMV strain TB40 with a plasmid carrying the TB40 UL32 gene fused to GFP. Because GFP is associated with a structural protein, virus particles fluoresce green under appropriate illumination; this is useful for following intracellular traffic of virus particles.
Product category
Viruses
Product type
Animal virus
Classification
Herpesviridae, Cytomegalovirus, Human betaherpesvirus 5
Strain designation
UL32-EGFP-HCMV-TB40
Common name
Cytomegalovirus
Isolation source
In vitro recombination of HCMV strain TB40 with a plasmid carrying the TB40 UL32 gene fused to GFP.
Applications
Infectious disease research
Product format
Frozen
Storage conditions
-70°C or colder
General
Specific applications
Tracking intracellular traffic of virus particles.
Characteristics
Comments
Recombinant HCMV virus with GFP fused to the C terminus of the tegument phosphoprotein pp150, the product of the UL32 gene. Because GFP is associated with a structural protein, virus particles fluoresce green under appropriate illumination. Useful for following intracellular traffic of virus particles.
Handling information
Host
MRC-5 (ATCC CCL-171)
Effects
CPE; cell rounding; cell enlargement
Complete medium
EMEM (ATCC 30-2003) supplemented with 2% FBS (ATCC 30-2020)
Temperature
37°C
Incubation
7-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (eg, 1 mL per 25 cm2) diluted to provide an optimal MOI (eg, 0.1 to 1.0). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
Recombinant HCMV virus with GFP fused to the C terminus of the tegument phosphoprotein pp150, the product of the UL32 gene. Because GFP is associated with a structural protein, virus particles fluoresce green under appropriate illumination. Useful for following intracellular traffic of virus particles.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Quality control specifications
History
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