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Enterovirus A71
VR-1775 ™
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Enterovirus A71 strain BrCr is propagated in Vero cells (ATCC CCL-81). This strain was originally isolated from the stool specimen of a 2-month-old male with aseptic meningitis and was derived by passage in Vero cells. It has applications in enteric disease research.
Product category
Viruses
Product type
Animal virus
Classification
Enterovirus, Enterovirus A, Enterovirus A71
Strain designation
BrCr
Genome sequenced strain
Yes
Isolation source
Stool specimen from 2-month-old male with aseptic meningitis. Derived by passage of ATCC VR-784 in Vero cells (ATCC CCL-81). This product replaces ATCC VR-784. and exhibits CPE when cells are infected.
Applications
Enteric disease research
Infectious disease research
Product format
Frozen
Storage conditions
-70°C or colder
General
Specific applications
Enteric Research
Characteristics
Comments
Derived by passage of ATCC VR-784 in Vero cells (ATCC CCL-81). This product replaces ATCC VR-784 and exhibits CPE when cells are infected.
Note: The sequences of ATCC VR-784 and ATCC VR-1775 have not been compared.
Mycoplasma contamination
Not detected
Handling information
Host
Vero (ATCC CCL-81)
Effects
CPE; cell degeneration; cell rounding; cell sloughing
Complete medium
EMEM (ATCC 20-2003) + 2% FBS (ATCC 30-2020)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
Incubate infected culture for 5-9 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Recommendation for infection
Plant cells 24-48 hours in advance and infect when cultures are 70-80% confluent. Remove medium, wash cell monolayer once with PBS, and inoculate with a small volume (eg. 1 mL per cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
Derived by passage of ATCC VR-784 in Vero cells (ATCC CCL-81). This product will replace ATCC VR-784. (Note: The sequences of ATCC VR-784 and ATCC VR-1775 have not been compared).
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
CPE, Cytopathic effect
MOI, Multiplicity of infection
Quality control specifications
History
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